Mutant for improving thermal stability of hexotensin I by increasing disulfide bond and preparation method thereof

A technology of thermal stability and mutant, applied in the fields of genetic engineering and enzyme engineering, can solve problems such as being unable to adapt to industrial application well, poor thermal stability of heparinase I, etc., and achieves reduced screening workload and excellent thermal stability performance. , the effect of good thermal stability

Active Publication Date: 2019-05-03
TIANJIN UNIV OF SCI & TECH +1
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Problems solved by technology

[0004] At present, the thermal stability of heparanase I is very poor, which makes it unable to adapt to industrial applications well

Method used

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  • Mutant for improving thermal stability of hexotensin I by increasing disulfide bond and preparation method thereof
  • Mutant for improving thermal stability of hexotensin I by increasing disulfide bond and preparation method thereof
  • Mutant for improving thermal stability of hexotensin I by increasing disulfide bond and preparation method thereof

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Embodiment Construction

[0039] The present invention will be described in further detail below in conjunction with specific examples. The following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0040] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0041] Unmarked test methods in the implementation of the present invention, such as the preparation of competent state, heat shock transformation plasmid and Escherichia coli Rosetta (DE3) medium, etc., all belong to routine operation methods, and can refer to the third edition of "Molecular Cloning Experiment Guide" in the method.

[0042] A mutant BtHepI that improves the thermostability of heparanase I by increasing disulfide bonds D204C / K208C , the amino acid sequence of the mutant is: SEQ ID NO.1....

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Abstract

The present invention relates to a mutant BtHepID204C / K208C for improving thermal stability of hexotensin I by increasing disulfide bond, and an amino acid sequence of the mutant is SEQ ID NO. 1. Themutant has better thermostability than wild-type heparinase I (BtHepI), and can effectively reduce the screening workload of a mutant library, at the same time, the thermostable heparinase I has greatsignificance for extending the shelf life of heparinase I, improving its operational stability and reusing batches in a catalytic process, , and reducing production costs. The industrial applicationvalue of heparinase I can be improved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering and enzyme engineering, in particular to a mutant and a preparation method for improving the thermal stability of heparanase I by increasing disulfide bonds. Background technique [0002] Heparin is a specific polydisperse mixed sulfated polysaccharide, which is widely distributed in mammalian tissues and binds to proteins in the form of covalent bonds. At present, commercial heparin is mainly extracted from bovine lung and pig small intestinal mucosa. It has a complex structure and has a variety of important biological functions. It is generally used clinically for the treatment of thrombosis, cardiovascular and other diseases. Low molecular weight heparin (Low molecular heparin, LMWH) is a small segment of heparin produced by cleavage of heparin by certain physical and chemical methods. The ability to bind to proteins or cells is reduced, but the anticoagulant activity is significant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N15/60C12N15/70C12Q1/527G01N21/31
Inventor 罗学刚张川张同存刘耀天
Owner TIANJIN UNIV OF SCI & TECH
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