High-throughput breeding method of ultrahigh-concentration beer brewing strain

A high-throughput, ultra-high-concentration technology, applied in the direction of microorganism-based methods, methods using microorganisms, biochemical equipment and methods, etc., can solve problems such as abnormal morphology, decreased vitality of brewer's yeast, aging and death, and improve work efficiency. Efficiency and targeting, reducing the intensity of screening work, and shortening the screening cycle

Pending Publication Date: 2022-05-17
BEIJING YANJING BREWERY
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Problems solved by technology

[0006] The purpose of the embodiments of the present invention is to provide a high-throughput breeding method for ultra-high-concentration beer brewing strains, aiming to solve the problem that beer yeast is the core of beer brewing, and under high-concentration stress, beer yeast has decreased vitality, abnormal shape, Metabolic abnormalities, aging and death, and even autolysis

Method used

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  • High-throughput breeding method of ultrahigh-concentration beer brewing strain
  • High-throughput breeding method of ultrahigh-concentration beer brewing strain
  • High-throughput breeding method of ultrahigh-concentration beer brewing strain

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Embodiment

[0033] Example: such as figure 1 and 2 Shown is a high-throughput breeding of an ultra-high-concentration brewing strain of below-fermenting yeast.

[0034] 1. ARTP mutagenesis of the starting strain to build a mutation library: pick a single colony into 5mL 16°P wort medium, 37°C, 250r / min, culture for 24h; prepare the bacterial suspension, select the appropriate mutagenesis condition as power 120w, gas volume 10slm, time is 30-45s, according to figure 1 The ARTP mutagenesis process shown in the figure mutagenizes the starting strain to obtain an ultra-high concentration strain mutant library, and obtains 50 plates (30-40 colonies per plate) after the mutagenizing of the starting strain;

[0035] 2. Determination of conditions for the high-throughput screening method based on droplet microfluidic technology: pick a single colony into 2mL 16°P wort medium, culture at 25°C, 250r / min; transfer to 18 °P wort medium (without adding alcohol), the culture solution was transferred...

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Abstract

The invention is applicable to the technical field of microbial fermentation engineering, and provides a high-throughput breeding method of an ultrahigh-concentration beer brewing strain, which comprises the following steps: step 1, obtaining an ultrahigh-concentration strain mutant library by using an ARTP mutagenesis technology; 2, breeding an ultrahigh-concentration brewing strain by using a high-throughput screening method based on a droplet microfluidic technology; 3, MMC micro-fluidic breeding and strain adaptability domestication are carried out; 4, gradient plate breeding; step 5, secondary screening of glucose metabolism power; and 6, analyzing the brewing performance of the optimized strain. According to the method, the number of strains screened each time is effectively increased, the screening period is shortened, the screening working intensity is reduced, the working efficiency and objectivity of the whole screening are improved, and the ultrahigh-concentration brewing strains suitable for the following fermentation are obtained.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation engineering, and in particular relates to a high-throughput breeding method for ultra-high-concentration beer brewing strains. Background technique [0002] Beer ultra-high-concentration brewing (wort concentration 18-25°P) refers to the technology of fermenting with a higher concentration of wort than the normal concentration in the beer production process, and diluting it with water into beer of regular concentration in the later stage of fermentation. [0003] Atmospheric and room temperature plasma (ARTP) is a new type of mutagenesis technology that utilizes plasma jets with a high concentration of active particles produced under atmospheric pressure in recent years. It has excellent characteristics such as simple operation, simple equipment, mild conditions, high safety and rapid mutagenesis. The mutagenesis effect on fungi such as brewer's yeast will be more obvious than conv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12N1/18C12N1/36C12R1/865
CPCC12N15/01C12N13/00C12N1/18C12N1/36
Inventor 贺秀丽谢鑫郭立芸侍亚敏付晓芬穆英健侯红霞贾凤超
Owner BEIJING YANJING BREWERY
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