In-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium

A technology for mutation breeding and Chu chrysanthemum, which is applied in the field of chemical mutation breeding technology, can solve the problems of delayed flowering period, inability to apply, increase in the number of chromosomes, etc., and achieves the effects of good effect, simple operation procedure and high frequency of induced mutation.

Inactive Publication Date: 2013-04-17
ANHUI SCI & TECH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the obtained mutants have increased number of chromosomes, which delay the flowering period, so they cannot be used in the improvement of Chuchrysanthemum varieties

Method used

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  • In-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium
  • In-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium
  • In-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] ①Wash and rinse the young stems of Chuju chrysanthemum with neutral detergent, then disinfect the surface with 75% ethanol for 30-90sec, and then wash with 0.1%HgCl 2 Sterilize the solution for 6-10 minutes, rinse the sterilized young stems with sterile water 4-5 times, and inoculate the explants of the stems with axillary buds in MS+NAA 0.1mgL -1For the culture medium, 2-3 explants are inoculated in each bottle, and can be subcultured once every 20-30 days to form a large number of sterile seedlings.

[0039] ②Choose aseptic seedlings grown for 15d-20d and cut them into stems with axillary buds and plant them in MS+TDZ 0.1-0.2mgL -1 +NAA.01 -0.2mgL -1 +NaN 3 4-8mgL -1 or MS+KT 3-4mgL -1 +NAA.01-0.4mgL -1 + NaN 3 4-8mgL -1 On the clump bud differentiation medium, 35d-45d part of the shoot tips, leaf axils or stem bases formed cluster buds.

[0040] ③Transfer the cluster buds formed by the mutagenesis treatment to MS+NAA 0.1mgL -1 On the strong seedling medium...

Embodiment 2

[0044] ①Wash and rinse the young stems of Chuju chrysanthemum with neutral detergent, then disinfect the surface with 75% ethanol for 30-90sec, and then wash with 0.1%HgCl 2 Sterilize the solution for 6-10 minutes, rinse the sterilized young stems with sterile water for 4-5 times, peel off the shoot tip explants and inoculate in MS+NAA 0.1mgL -1 For the culture medium, 2-3 explants are inoculated in each bottle, and can be subcultured once every 30-40 days to form a large number of sterile seedlings.

[0045] ②Choose aseptic seedlings grown for 15d-20d and cut them into stems with axillary buds and inoculate them in MS+TDZ 0.1-0.2mgL -1 + NAA.01-0.2mgL -1 +NaN 3 4-8mgL -1 or MS+KT 3-4mgL -1 +NAA.01-0.4mgL -1 + NaN 3 4-8mgL -1 On the clump bud differentiation medium, 35d-45d part of the shoot tips, leaf axils or stem bases formed cluster buds.

[0046] ③Transfer the cluster buds formed by the mutagenesis treatment to MS+NAA 0.1mgL -1 On the strong seedling medium, cu...

Embodiment 3

[0050] a. Wash and rinse the young leaves of Chrysanthemum chrysanthemum with neutral detergent, then disinfect the surface with 75% ethanol for 30-90sec, and then wash with 0.1% HgCl 2 Sterilize the solution for 6-10 minutes, rinse the sterilized young leaves 4-5 times with sterile water, cut the young leaves into small pieces of about 0.5×0.3cm2, and inoculate them into MS+TDZ 0.1-0.2mgL -1 +NAA.01-0.2mgL -1 +NaN 3 4-8mgL -1 or MS+KT 3-4mgL -1 +NAA.01-0.4mgL -1 + NaN 3 4-8mgL -1 On the cluster bud differentiation medium, inoculate 2-3 explants in each bottle, culture for 40-50 days, and 20-30% of the leaf explants form cluster buds.

[0051] b. Cut the aseptic seedlings of cluster buds into stems with axillary buds and inoculate them in MS+TDZ 0.1-0.2mgL -1 +NAA.01-0.2mgL -1 +NaN 3 4-8mgL -1 or MS+KT 3-4mgL -1 +NAA.01-0.4mgL -1 + NaN 3 4-8mgL -1 On the clump bud differentiation medium, 35d-45d part of the shoot tips, leaf axils or stem bases formed cluster ...

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Abstract

The invention relates to a chemical mutation breeding technology in the field of biotechnologies, and particularly relates to an in-vitro sodium azide (NaN3) mutation breeding technology of dendranthema morifolium. The in-vitro NaN3 mutation breeding technology comprises the following steps of: performing culture in vitro on an immature stem, a stem tip or a leaf of the dendranthema morifolium to form an aseptic seedling; processing the stem tip and a stem segment of the aseptic seedling, which are taken as explants, by sodium azide (NaN3) in a breeding process to carry out induced mutation; rooting and transplanting a test-tube plantlet; and finally breeding excellent mutant in a field at a flowering phase. The in-vitro sodium azide (NaN3) mutation breeding technology can effectively overcome the obstacle of breed improvement of a clonal plant and the constraints of low bud-variation frequency and small mutational range under natural conditions, can be used for obtaining the excellent mutant within a short period of time, ensures high induced mutation efficiency, and has an important value to the genetic improvement of asexual propagation of the dendranthema morifolium with medicinal and ornamental values.

Description

technical field [0001] A kind of Chuju isolated NaN 3 The mutation breeding technology belongs to the chemical mutation breeding technology in the field of biotechnology. Background technique [0002] Chuju Dendranthema morifolium 'Chuju', Asteraceae Asteraceae, chrysanthemum genus Chrysanthemum perennial herb, is one of the four major medicinal chrysanthemums in China, and a famous authentic medicinal material in Anhui. It uses flowers as medicine. It is an important traditional Chinese medicine. It is a good product for both medicine and tea. It has the functions of clearing heat and detoxifying, relaxing tendons and activating blood circulation, protecting liver and improving eyesight, and enhancing human immune function. It has a significant effect on hypertension, coronary heart disease and arteriosclerosis. In recent years, studies have found that chrysanthemum has a good preventive effect on cancer, etc., and has an obvious therapeutic effect on diabetes. Chuzhou c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张子学许峰胡能兵周玉丽何克勤林平
Owner ANHUI SCI & TECH UNIV
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