Method for rapidly obtaining high-nicotine tobacco mutant by screening tobacco root systems

Abstract

The invention provides a method for rapidly obtaining a high-nicotine tobacco mutant by screening tobacco root systems. Yunyan 87 mutation second-generation seeds and Yunyan 87 control seeds are subjected to spot seeding respectively, after the Yunyan 87 control seeds grow out two true leaves, Yunyan 87 mutation second-generation materials and Yunyan 87 control material seedlings are pulled out and cleaned to compare the root systems, and a mutant material KH65 with the more developed root systems is screened out and cultured and transplanted; after tobacco plants are mature, upper, middle andlower tobacco leaves are sampled to measure the nicotine content of a single plant KH65; after the tobacco plant KH65 blooms, selfing is conducted to collect seeds, and the seeds are sown again in the second year; the root system conditions of the mutant material are identified again, and after the tobacco plant is mature, sampling is conducted again to measure the nicotine content of the maturemutant material of the single plant; after the mature mutant tobacco plant grow to bloom, selfing is conducted to collect seeds, and the high-nicotine tobacco mutant is obtained. According to the method, the tobacco mutant material high in nicotine content can be rapidly identified and obtained by screening the root expression of the tobacco EMS mutant material.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for rapidly screening high-nicotine tobacco mutants. Background technique [0002] Alkaloids are the most important class of secondary metabolites in tobacco. The alkaloids in tobacco mainly include Nicotine (Nicotine), Nornicotine (Nornicotine), Anatibine and Anabasine. Nicotine is the most important secondary metabolite with the highest content in cultivated tobacco (Nicotiana tabacum). Nicotine, as the most well-known alkaloid, is the most economically valuable part of tobacco. The amount of content can directly determine the quality of tobacco leaves. The alkaloids in all known tobacco varieties currently account for about 2%-4% of the dry weight, and nicotine can account for 90%-95% of the total tobacco alkaloids. [0003] Nicotine can respond to biotic and abiotic stress, and is an important means for tobacco to resist external stress. However, high nicotine conten...

AI Technical Summary

Problems solved by technology

[0010] In order to solve the problem of insufficient nicotine content in the production process of cigarettes, the object of the present invention is to provide a method for quickly obtaining high-nicotine tobacco mutants by screening tobacco roots. Identify tobacco mutant materials with high nicotine content to greatly reduce the workload of screening high-nicotine mutants, save time and money, and improve production efficiency

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