Method for screening high-nicotine tobacco variety mutants

A technology of high nicotine and mutants, applied in the biological field, can solve problems such as long cultivation time, uncertainty of transgenic safety, and linkage of traits

Inactive Publication Date: 2020-07-28
CHINA TOBACCO HENAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The purpose of the present invention is to provide a method for screening mutants of high-nicotine and high-secretion tobacco varieties, so as to solve the problems of t...

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  • Method for screening high-nicotine tobacco variety mutants
  • Method for screening high-nicotine tobacco variety mutants
  • Method for screening high-nicotine tobacco variety mutants

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[0052] The technical solution of the present invention is described in detail below through the examples, and the following examples are only exemplary and can only be used to explain and illustrate the technical solution of the present invention, rather than being interpreted as a limitation to the technical solution of the present invention.

[0053] Each embodiment of this application is based on the cultivation of the normal China Tobacco 100 variety. Due to space constraints, other varieties cannot be explained one by one, but it does not mean that the technical solution of this application is only applicable to the China Tobacco 100 variety. Breeding, for actual needs, other flue-cured tobacco varieties can be selected and bred according to the technical scheme. The screening of the present application includes two methods, one is to use only one EMS induction method to process, and then the plants of the treated seeds are detected, and the second is to conduct the obtained...

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Abstract

The invention relates to a method for screening high-nicotine tobacco variety mutants. According to the method, mutagenized plants are created by the EMS mutagenesis method, leaf glandular hair morphology screening and leaf secretion determination are performed to select first mutant strains meeting first set criteria, and M3 generation seeds are harvested for cultivation; the Tilling technology is used to screen mutant single plants of a nicotine synthesis negative regulation gene NtJAZ1, continuous backcross and self-cross are carried out between the mutant single plants and tobacco varieties to be screened as recurrent parents, and mutant strains with high-nicotine and high-secretion are obtained with the help of the molecular marker and gene chip-assisted selection technology; and comparative studies on agronomic traits, comprehensive resistance, chemical composition, sensory quality and the like of the mutant strains are performed, new varieties with increased nicotine content, excellent comprehensive traits, and significantly-improved tobacco aroma are selected from the mutant strains, and thereby the foundation for the production of highly-flavored high-quality tobaccos is laid.

Description

technical field [0001] The invention belongs to the field of biotechnology, and belongs to the cultivation technology of new tobacco varieties, in particular to a method for screening mutants of high-nicotine and high-secretion tobacco varieties. Background technique [0002] Nicotine content is one of the important factors to measure the quality of tobacco leaves, which directly affects the physiological strength, smoke characteristics and safety of tobacco products. Nicotine is the source of the taste of tobacco leaves, which can stimulate the smokers' sense of taste and enable them to obtain physiological satisfaction. At the same time, nicotine also has an important impact on the aroma quality of tobacco leaves. Pyrolysis of nicotine can produce aroma components of pyridine compounds in smoke. Within a certain range, appropriately increasing the nicotine content can endow tobacco leaves with better physiological strength and aroma quality. The nicotine content in tobacc...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/04
CPCA01H1/06A01H1/04
Inventor 杨欣玲王新中姚倩张子颖申洪涛张书伟彭玉富
Owner CHINA TOBACCO HENAN IND
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