Pectinase produced through mixed bacterium fermentation, and application thereof in tobacco sheet processing

A technology of mixed bacteria fermentation and tobacco flakes, applied in the application, tobacco, tobacco processing and other directions, can solve the problems of poor pertinence of pectin degradation, difficult to control the degradation process, and insufficient degradation.

Inactive Publication Date: 2015-07-01
CHINA TOBACCO HENAN IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the complexity and diversity of the pectin structure in the tobacco sheet, pectinases in the prior art generally have the defects that the degradation of pectin in the tobacco sheet is not targeted, the degradation is not sufficient, and the degradation process is difficult to control. Urgent need to explore new pectinases and their application in tobacco sheet processing

Method used

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  • Pectinase produced through mixed bacterium fermentation, and application thereof in tobacco sheet processing
  • Pectinase produced through mixed bacterium fermentation, and application thereof in tobacco sheet processing
  • Pectinase produced through mixed bacterium fermentation, and application thereof in tobacco sheet processing

Examples

Experimental program
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Embodiment 1

[0028] This embodiment mainly briefly introduces the process of producing pectinase by mixed bacteria fermentation and the optimization process of related medium as follows.

[0029] The pectinase produced by mixed bacteria fermentation is obtained by the following steps:

[0030] (1) Activation of strains , picking the microbial strains from the preservation medium, inoculating them into the activation medium, and culturing;

[0031] The microbial strains include: the Aspergillus niger (Aspergillus niger) with the preservation number of 3.3287 preserved by the China General Microorganism Culture Collection Center (CGMCC), and the preservation number of 1012 preserved by the China Industrial Microbiology Culture Collection Center (CICC). Saccharomyces cerevisiae; both strains were purchased from corresponding depository institutions;

[0032] The activation medium of Aspergillus niger is PDA medium (potato 300g / L, glucose 20g / L, agar 20g / L, natural pH, sterilized at 121°C),...

Embodiment 2

[0066] In this example, the crude enzyme solution prepared in Example 1 was used to degrade tobacco flakes, thereby reducing the pectin content in tobacco flakes, and further sensory evaluation and analysis were carried out. The brief introduction is as follows.

[0067] The application of the pectinase produced by mixed bacteria fermentation in Example 1 in the tobacco sheet processing process, after diluting the obtained crude enzyme solution 0 to 20 times (theoretically, the crude enzyme solution with any enzyme activity can be used to degrade pectinase) Gelase, this example only takes the crude enzyme solution with the highest enzyme activity as an example, that is, the enzyme activity is 3228.86U / mL, to discuss the degradation effect of pectinase and the improvement of tobacco quality), and carry out biological Degradation treatment. The specific process is:

[0068] The crude enzyme liquid was evenly sprayed on 10 g of tobacco flakes with a small sprayer at different ma...

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Abstract

The invention relates to the technical field of tobacco preparation, and in particular relates to a pectinase produced through mixed bacterium fermentation, and an application thereof in tobacco sheet processing. The pectinase is obtained through the steps of strain activation, seed liquid preparation, fermentation culture, enzyme solution crude extraction, and the like. The crude enzyme solution is diluted by 0-20 times, and the diluted crude enzyme solution is used for treating tobacco sheets by enzymolysis for 2-6h at a temperature of 40-50 DEG C according to a ratio that tobacco sheet to pectinase is 1:(1-5). An overall technical route of the application comprises the following steps: specific strains are mixed and fermented, wherein an optimal fermentation condition is obtained by screening and optimization, such that the crude enzyme solution with highest pectinase enzyme activity is obtained; the crude enzyme solution is used for treating tobacco sheets, such that pectin content in the tobacco sheets can be reduced, and a best degradation effect can be obtained. The maximal enzyme activity of the crude enzyme solution provided by the invention can be higher than 3200U / mL. When the crude enzyme solution is used in tobacco sheet treatment, the pectin content in the tobacco sheets can be reduced by approximately 1 / 4, and tobacco smoking quality can be well improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco preparation, and in particular relates to a pectinase produced by mixed bacteria fermentation and its application in the process of tobacco sheet processing. Background technique [0002] Pectinase is a complex enzyme composed of various components, each component can synergistically complete the degradation of pectin, and has a wide range of uses in food processing, feed processing, textile, papermaking, environmental protection, etc. The production of pectinase has a history of more than 40 years, and there are mainly two production methods: solid-state fermentation method and liquid fermentation method. Many microorganisms have the ability to produce pectinase. In the prior art, pectinase-producing enzyme-producing strains are mainly fungi, and Aspergillus species are widely used. Generally speaking, the pectinase activity produced by the solid-state fermentation method is relatively high, but...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N9/26C12N9/18A24B15/20C12R1/685C12R1/865
CPCC12N9/2402A24B15/20C12N9/18C12N9/88C12Y301/01011C12Y302/01015C12Y402/02002
Inventor 马宇平郝辉许春平孙斯文聂聪王文领
Owner CHINA TOBACCO HENAN IND
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