Method for separating carnosic acid from rosemary leaf extract

Abstract

The invention discloses a method for separating carnosic acid from a rosemary leaf extract. The method comprises the following steps of: dispersing column chromatography silica gel in a phosphoric acid-ethanol solution, vacuumizing, ultrasonically soaking, recycling ethanol and drying to obtain a phosphoric acid-silica gel stationary phase; filling the phosphoric acid-silica gel stationary phase into a chromatography column tube, imported with high-purity nitrogen for protection, to obtain a phosphoric acid-silica gel chromatography column; and dissolving the rosemary leaf extract in ethyl acetate, adding the phosphoric acid-silica gel stationary phase into the mixture, fully stirring to recycle the ethyl acetate to obtain a stationary phase adsorbing the rosemary leaf extract, adding thestationary phase adsorbing the rosemary leaf extract into the phosphoric acid-silica gel chromatography column, eluting, collecting fractions and performing vacuum drying to obtain carnosic acid. By adopting the method, the carnosic acid is separated independently only by once column chromatography, the column chromatography time is short, the purity is up to 92 percent, and the application of a product in the market is promoted.

Description

technical field

[0001] The invention relates to a method for separating carnosic acid from rosemary leaf extract. Background technique

[0002] Rosemary (Rosmarinus offoconalis L.) is a plant of the genus Rosmarinus of the Lamiaceae, rich in a variety of efficient and safe antioxidant components. Rosemary extract is a new type of excellent natural antioxidant, which can effectively remove free radicals in the body, prevent food from deteriorating, and has anti-inflammatory, anti-tumor, and anti-HIV effects ([1] Yang Yan. Rosemary Extract Properties and applications of substances [J]. China Food Industry, 1999, (3): 38-39. [2] OffordEA, MacéK, Avanti O, et al. Cancer Letters [J]. 1997, 114 (12): 275 -281.). Carnosic acid (CA) is the most important active ingredient in rosemary fat-soluble antioxidants. It not only has antioxidant activity, but also has anticancer properties. It is widely used in cosmetics, medicines and health products.

[0003] At present, there are mainl...