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Application of carnosic acid in preparing medicament for suppressing angiogenesis

An angiogenesis and carnosic acid technology, applied in drug combinations, cardiovascular system diseases, anti-tumor drugs, etc., to achieve the effect of inhibiting tubule formation

Inactive Publication Date: 2011-05-18
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the application of carnosic acid in inhibiting angiogenesis

Method used

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  • Application of carnosic acid in preparing medicament for suppressing angiogenesis
  • Application of carnosic acid in preparing medicament for suppressing angiogenesis
  • Application of carnosic acid in preparing medicament for suppressing angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Carnosic acid inhibits the proliferation of human umbilical vein endothelial cells (HUVEC)

[0031] The proliferation of HUVEC was detected by CCK-8 method. CCK-8 reagent contains WST–8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene) -2H-tetrazolium monosodium salt), which is reduced by the dehydrogenase in the mitochondria of the cell under the action of the electron carrier 1-Methoxy PMS (1-methoxy-5-methylphenazine dimethyl sulfate) to have Highly water-soluble yellow formazan (Formazan) product. The amount of formazan produced is directly proportional to the number of viable cells. The light absorption value was measured at a wavelength of 450 nm by an enzyme-linked immunosorbent detector, which can indirectly reflect the number of living cells. The effect of drugs on the proliferation of HUVCE can be evaluated by this method.

[0032] Methods: HUVEC cells were seeded in 96-well plates and cultured. After the cell grow...

Embodiment 2

[0036] Example 2 Carnosic acid inhibits the migration of human umbilical vein endothelial cells (HUVEC)

[0037] Transwell method was used to detect HUVEC migration. Transwell is an experimental technology. The main material of this technology is the Transwell chamber (chamber). The bottom layer of the chamber has a layer of permeable membrane (polycarbonate membrane (polycarbonate membrane) is commonly used, and this membrane has pores. Micropores with a size of 0.1-12.0 μm. In the experiment, the cells are placed on the small chamber. Under the action of the chemokines or growth factors contained in the small chamber, the cells pass through the polycarbonate membrane, thereby performing co-culture, cell chemotaxis, cell Migration, cell invasion and many other studies.

[0038] Method: Add 600 μL of VEGF containing 20 ng / ml to the lower chamber 165 HUVEC culture medium. HUVEC containing different concentrations of carnosic acid were added to the upper chamber (the cell d...

Embodiment 3

[0040] Example 3 Carnosic acid inhibits HUVEC tubule formation experiment

[0041] The tubule formation of HUVEC was detected by Matrigel gel. Artificial basement membrane Matrigel is a basement membrane component extracted from mouse EHS sarcoma. It can spontaneously form a gel-like artificial basement membrane at 37 °C, and has the biological function of a natural basement membrane. Human umbilical vein endothelial cells can adhere to form tubes on Matrigel glue, which can be used to study the effect of drugs on the tube formation of endothelial cells.

[0042] Method: 50 μl of Matrigel gel solution was added to a pre-cooled 96-well culture plate, and then placed in a 37 °C incubator for 1 h to solidify the gel. Add HUVECs (cell density 1×105 / ml) containing different concentrations of carnosic acid into Matrigel-coated 96-well plates and place in CO 2 Incubate for 10 h in the cell culture incubator. The group without carnosic acid was used as the control. After 10 h, b...

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Abstract

The invention relates to application of carnosic acid in preparing a medicament for suppressing angiogenesis. The invention has the advantages that: the novel application of the carnosic acid discovers the effect of the carnosic acid in resisting angiogenesis for the first time, and the carnosic acid can effectively suppress proliferation, migration and canaliculization of vascular endothelial cells for the first time, which proves that the carnosic acid has the activity of resisting the angiogenesis, can be used as an angiogenesis suppressant, and can be applied to preparation of angiogenesis medicaments as the angiogenesis suppressant to treat neovascularization dependent and neovascularization related diseases, such as tumors, arthritis, psoriasis, ocular diseases, atherosclerosis, and the like.

Description

technical field [0001] The invention relates to the application of a Chinese herbal medicine monomer, in particular to the application of a Chinese herbal medicine monomer carnosic acid in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis refers to the process in which new blood vessels form mature blood vessels at all levels through remodeling and expansion. New vessels often sprout out from existing mature tissue, or split into smaller daughter vessels from a large vasculature. Angiogenesis mainly includes the following processes: (1) Degradation of vascular basement membrane during the activation phase; (2) Activation, proliferation, and migration of vascular endothelial cells; (3) Formation of new capillary lumen structure; (4) Recruitment of pericytes to Stabilize the newly formed capillary network; (5) form a mature vascular network through the remodeling and expansion of primitive blood vessels. Angiogenesis plays an im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/192A61P35/00A61P35/02A61P27/02A61P19/02A61P17/06A61P9/10A61P43/00
Inventor 方超陈红专赵梅陆琴王超於得红白帆
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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