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Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis

A technology of dehydroxylactone and angiogenesis, which can be used in drug combination, cardiovascular system diseases, anti-tumor drugs, etc. to achieve the effect of inhibiting proliferation

Inactive Publication Date: 2012-08-29
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the application of dehydrocressin in inhibiting angiogenesis.

Method used

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  • Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis
  • Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis
  • Application of dehydrocostuslactone to preparing drug for inhibiting angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Dehydrocostuslactone inhibits the proliferation of human umbilical vein endothelial cells (HUVEC)

[0032] The CCK-8 method was used to detect the proliferation of HUVEC. CCK-8 reagent contains WST-8(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene) -2H-tetrazole monosodium salt), which is reduced by dehydrogenase in the mitochondria of the cell under the action of the electron carrier 1-Methoxy PMS (1-methoxy-5-methylphenazine dimethyl sulfate) to have Highly water-soluble yellow formazan (Formazan) product. The amount of formazan produced is directly proportional to the number of living cells. The light absorption value measured at 450 nm with an enzyme-linked immunoassay can indirectly reflect the number of living cells. This method can be used to evaluate the effects of drugs on the proliferation of HUVCE.

[0033] Method: HUVEC cells were seeded and cultured in 96-well plates. After the cells have grown to 80% confluence, add ...

Embodiment 2

[0037] Example 2 Dehydrocostuslactone inhibits migration of human umbilical vein endothelial cells (HUVEC)

[0038] Transwell method detects HUVEC migration. Transwell is an experimental technology. The main material of this technology is the Transwell chamber. The bottom layer of the chamber has a permeable membrane (polycarbonate membrane is commonly used, which has pores). Micropores with a size of 0.1-12.0 μm. In the experiment, the cells are placed on the chamber. Under the action of the chemokine or growth factor contained in the chamber, the cells penetrate the polycarbonate membrane for co-culture, cell chemotaxis, and cell Research on migration and cell invasion.

[0039] Method: Add 600 μL of VEGF containing 20 ng / ml to the lower chamber 165 HUVEC culture medium. HUVEC containing different concentrations of dehydrocostuslactone (cell density of 2×10 5 / ml), placed in CO 2 Culture in a cell incubator for 8 hours. The group without dehydrocostus lactone was used as a co...

Embodiment 3

[0041] Example 3 Dehydrocostuslactone inhibits HUVEC tubule formation experiment

[0042] Matrigel glue was used to detect the tubule formation of HUVEC. Artificial basement membrane Matrigel glue is a basement membrane component extracted from mouse EHS sarcoma. It can spontaneously form a gel-like artificial basement membrane at 37 ℃, which has the biological function of natural basement membrane. Human umbilical vein endothelial cells can be adhered to Matrigel glue into tubes, which can be used to study the effect of drugs on the formation of endothelial cells.

[0043] Method: Take 50 μl of Matrigel glue solution and add it to a pre-cooled 96-well culture plate, then place it in a 37 ℃ incubator and incubate for 1 h to solidify the glue. The HUVEC containing different concentrations of dehydrocostuslactone (cell density of 1×10 5 / ml) were added to the 96-well plate covered with Matrigel and placed in CO 2 Culture in a cell incubator for 10 h. The group without dehydrocost...

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Abstract

The invention relates to application of dehydrocostuslactone to preparing a drug for inhibiting angiogenesis. The invention has the advantages of providing the novel application of the dehydrocostuslactone, finding the anti-angiogenesis of the dehydrocostuslactone for the first time, finding that the dehydrocostuslactone can effectively inhibit the multiplication, the migration and the canaliculization of vascular endothelial cells for the first time, showing that the dehydrocostuslactone has the activity of the anti-angiogenesis and can be used as an angiogenesis inhibitor as well as can be used as the angiogenesis inhibitor used for preparing the angiogenesis drug and treating vascularization dependent and vascularization relevant diseases of tumors, arthritis, parapsoriasis guttata, eye disorders, atherosclerosis and the like.

Description

technical field [0001] The invention relates to the application of a Chinese herbal medicine monomer, in particular to the application of a Chinese herbal medicine monomer dehydrocressin in the preparation of drugs for inhibiting angiogenesis. Background technique [0002] Angiogenesis is a complex process involving multiple molecules from multiple cells, including endothelial cell secretion of proteases to degrade basement membrane, proliferation and migration of endothelial cells, formation of new capillary luminal structures, and recruitment of pericytes to stabilize newly formed capillaries The mature vascular network is formed through the remodeling and expansion of primitive blood vessels. [0003] The dynamic balance between angiogenesis-inhibiting molecules and angiogenesis-promoting molecules is considered to be the "switch" that regulates angiogenesis. In most cases, the two are in balance, and the angiogenesis mechanism is turned off; when the balance of the two ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/365A61P35/00A61P35/02A61P17/06A61P19/02A61P9/10A61P27/02
Inventor 陈红专方超陆琴赵梅於得红白帆王超
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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