Method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves

Abstract

The invention relates to a method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves. The method mainly comprises the following steps that rosemary dry leaves are crushed and are then put into a water solution; temperature rise distillation is performed for 0.5h; distillates are collected to obtain the rosemary essential oil; after the distillation is completed, eight times of volume of 75-percent ethanol solution is added; extraction is performed for 2h at 60 DEG C; filtering is performed to obtain filtering liquid A; six times of volume of 75-percent ethanol solution is added into filtering slag; extraction is performed for 1h at 60 DEG C; filtering is performed to obtain filtering liquid B; the filtering liquid A andthe filtering liquid B are merged; concentration and centrifugation are performed to obtain a solid part and a water phase part; the water phase part is subjected to concentration spray treatment to obtain the rosemary acid; 60-percent ethanol solution is added into the solid part; after the dissolution, centrifugation is continuously performed and is repeated for three times to obtain the ursolicacid; the liquid part is subjected to concentration drying treatment to obtain the carnosic acid. The extracting method has the advantages that the process is simple; green and environment-friendly effects are achieved; the operation is convenient; the cost is low; the yield is high; the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to a method for extracting and separating antioxidants, the main active components of which are rosemary essential oil, rosmarinic acid, ursolic acid and carnosic acid. Background technique [0002] Antioxidants are used to prevent or delay the oxidation of animal and vegetable oils, oily foods, instant noodles, meat products and beverages, etc., to prolong the storage period of products, or to achieve the purpose of freshness. Antioxidants are mainly divided into natural antioxidants and synthetic antioxidants. Researchers have conducted high-dose animal experiments on synthetic antioxidants and natural antioxidants. The results show that synthetic antioxidants can not only significantly cause hepatomegaly, but also increase the activity of liver and lung microsomal enzymes, thereby making other substances ingested by the human body Transformation into toxic su...

AI Technical Summary

Problems solved by technology

[0010] Although the above methods have their own advantages, they also have some shortcomings. For example, they cannot simultaneously realize the classification and extraction of rosemary essential oil, rosmarinic acid, ursolic acid and carnosic acid in one production process, and the conditions are relatively harsh. It needs to be carried out under vacuum, toxic or acid-base conditions, the process is more complicated, the cost is higher, and it will also bring some adverse problems to the health of operators and the external environment

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