Method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves

A technology of rosemary essential oil and rosmarinic acid, which is applied in the fields of essential oils/perfumes, chemical instruments and methods, separation/purification of carboxylic acid esters, etc., can solve the problems of high cost, poor health of operators, and poor external environment, process complex and other issues, to achieve the effects of reduced production costs, high extraction efficiency, and simple extraction process

Inactive Publication Date: 2018-05-25
长沙卫一生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Although the above methods have their own advantages, they also have some shortcomings. For example, they cannot simultaneously realize the classification and extraction of rosemary essential oil, rosmarinic acid, ursolic acid and carnosic acid in one production ...

Method used

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  • Method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves
  • Method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves
  • Method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0031]Take by weighing the dried rosemary of 100kg, pulverize, place in 1000L (V / V) water, heat up and distill for half an hour, collect distillate, obtain 0.97kg rosemary essential oil; After distillation, in the rosemary leaf residue Add 800L (V / V) 75% ethanol solution, extract at 60°C for 2 hours, filter twice with ceramic membrane to obtain filtrate A; continue to add 600L (V / V) 75% ethanol solution to the filter residue, and extract at 60°C For 1 hour, filter twice with a ceramic membrane to obtain filtrate B; combine filtrate A and filtrate B, concentrate the filtrate until there is no alcohol smell, and centrifuge at a speed of 3000r / min to obtain the water phase and the solid part, and continue to concentrate the water phase Spray to obtain dry matter rosmarinic acid 9.85kg; Add 60% ethanol solution to the solid part, after dissolving, continue to centrifuge with the same rotating speed, repeat three times, after the gained solid part is dried, obtain 7.56kg ursolic aci...

Embodiment 2

[0036] Take by weighing the dried rosemary of 150kg, pulverize, place in 1500L (V / V) water, heat up and distill for half an hour, collect distillate, obtain 1.52kg rosemary essential oil; After distillation, in the rosemary leaf residue Add 1200L (V / V) 75% ethanol solution to the mixture, extract at 60°C for 2 hours, filter twice with a ceramic membrane to obtain filtrate A; continue to add 900L (V / V) 75% ethanol solution to the filter residue, Extract under the conditions for 1 hour, filter twice with a ceramic membrane to obtain filtrate B; combine filtrate A and filtrate B, concentrate the filtrate until it has no alcohol smell, and centrifuge at a speed of 3000r / min to obtain the water phase and the solid part, and the water phase Partially continue to concentrate and spray to obtain 14.64kg of dry matter rosmarinic acid; add 60% ethanol solution to the solid part, after dissolving, continue to centrifuge at the same speed, repeat three times, after the gained solid part is...

Embodiment 3

[0039] Take by weighing the dried rosemary of 200kg, pulverize, place in 2000L (V / V) water, heat up and distill for half an hour, collect distillate, obtain 1.92kg rosemary essential oil; After distillation, in the rosemary leaf residue Add 1600L (V / V) 75% ethanol solution, extract at 60°C for 2 hours, filter twice with ceramic membrane to obtain filtrate A; continue to add 1200L (V / V) 75% ethanol solution to the filter residue, extract at 60°C For 1 hour, filter twice with a ceramic membrane to obtain filtrate B; combine filtrate A and filtrate B, concentrate the filtrate until there is no alcohol smell, and centrifuge at a speed of 3000r / min to obtain the water phase and the solid part, and continue to concentrate the water phase Spray to obtain dry matter rosmarinic acid 20.11kg; Add 60% ethanol solution to the solid part, after dissolving, continue to centrifuge with the same rotating speed, repeat three times, after the gained solid part is dried, obtain 15.74kg ursolic ac...

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Abstract

The invention relates to a method for extracting and separating rosemary essential oil, rosemary acid, ursolic acid and carnosic acid from rosemary leaves. The method mainly comprises the following steps that rosemary dry leaves are crushed and are then put into a water solution; temperature rise distillation is performed for 0.5h; distillates are collected to obtain the rosemary essential oil; after the distillation is completed, eight times of volume of 75-percent ethanol solution is added; extraction is performed for 2h at 60 DEG C; filtering is performed to obtain filtering liquid A; six times of volume of 75-percent ethanol solution is added into filtering slag; extraction is performed for 1h at 60 DEG C; filtering is performed to obtain filtering liquid B; the filtering liquid A andthe filtering liquid B are merged; concentration and centrifugation are performed to obtain a solid part and a water phase part; the water phase part is subjected to concentration spray treatment to obtain the rosemary acid; 60-percent ethanol solution is added into the solid part; after the dissolution, centrifugation is continuously performed and is repeated for three times to obtain the ursolicacid; the liquid part is subjected to concentration drying treatment to obtain the carnosic acid. The extracting method has the advantages that the process is simple; green and environment-friendly effects are achieved; the operation is convenient; the cost is low; the yield is high; the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of natural product chemistry, and in particular relates to a method for extracting and separating antioxidants, the main active components of which are rosemary essential oil, rosmarinic acid, ursolic acid and carnosic acid. Background technique [0002] Antioxidants are used to prevent or delay the oxidation of animal and vegetable oils, oily foods, instant noodles, meat products and beverages, etc., to prolong the storage period of products, or to achieve the purpose of freshness. Antioxidants are mainly divided into natural antioxidants and synthetic antioxidants. Researchers have conducted high-dose animal experiments on synthetic antioxidants and natural antioxidants. The results show that synthetic antioxidants can not only significantly cause hepatomegaly, but also increase the activity of liver and lung microsomal enzymes, thereby making other substances ingested by the human body Transformation into toxic su...

Claims

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Application Information

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IPC IPC(8): C11B9/02C07C51/42C07C51/47C07C62/32C07C67/48C07C67/56C07C69/732C07J63/00
CPCC07C51/42C07C51/47C07C67/48C07C67/56C07J63/008C11B9/027C07C69/732C07C62/32
Inventor 刘喜荣罗成平何泽芳
Owner 长沙卫一生物科技有限公司
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