Agrimony total flavone extract, preparing method and applications thereof

A technology of extracts and total flavonoids, applied in the field of preparation of cardiovascular and cerebrovascular drugs, to achieve the effect of fewer procedures, shortened production cycle, and removal of harmful metals or metalloid elements

Inactive Publication Date: 2008-07-30
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no reports in the literature that the total flavonoids extract of Agrimony is used to treat myocardial infarction, angina pectoris, myocardial ischemia and other cardiovascular and cerebrovascular diseases and blood lipid-lowering effects

Method used

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  • Agrimony total flavone extract, preparing method and applications thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Extraction and Purification of Agrimony Total Flavonoids Extract

[0025] Add 10kg of Agrimony whole herb and 80L of water to the extraction tank, boil for 2 hours, drain the extract from the bottom of the extraction tank, add 50L of water and extract for 1.5 hours under the same conditions. The two extracts were combined by filtration. The extract was concentrated under reduced pressure and dried to obtain 2 kg of extract.

[0026] Add 10kg of Agrimony whole herb and 70L of 60% ethanol to the extraction tank, boil for 2 hours, release the extract from the bottom of the extraction tank, add 50L of 60% ethanol and extract for 1.5 hours under the same conditions. The two extracts were combined by filtration. The extract was concentrated under reduced pressure and dried to obtain 1.7kg of extract.

[0027]Disperse 50 g of extract in 0.5 L of water, extract with 0.5 L of n-butanol, repeat 3 times, combine the extracts to recover n-butanol under reduced pressure...

Embodiment 2

[0029] The content detection of embodiment 2 Agrimony total flavonoids extract

[0030] Preparation of standard curve: Accurately weigh 10.0000 mg of rutin standard substance and dissolve in 50% ethanol aqueous solution, and dilute to 25 mL to obtain 400 μg / mL rutin standard solution. Accurately measure 0.0, 0.5, 1.0, 1.5, 2.0, 2.5mL of the above rutin standard solution into a 10mL volumetric flask, add 50% ethanol aqueous solution to make up 5mL, add 5% NaNO successively 2 and 10% Al(NO 3 ) 3 0.3 mL each, mix and stand for 5 minutes, add 4 mL of 4% NaOH solution, and finally use 50% ethanol aqueous solution to make up the volume, mix and stand for 10 minutes, then measure the absorbance A at 510 nm. Get the standard curve regression equation: y=0.9756x+0.0018, R 2 =0.9995

[0031] Determination: Accurately weigh 6.0000 mg of Agrimony total flavonoid extracts A and B, dissolve them in 50% ethanol aqueous solution, and dilute to 10 mL to obtain a test solution of 600 μg / mL....

Embodiment 3

[0033] Antioxidant activity experiment of embodiment 3 Agrimony total flavonoids extract

[0034] (1) The role of scavenging DPPH free radicals in vitro

[0035] DPPH was prepared into a 0.65mM solution with absolute ethanol and diluted immediately before use. The concentration of DPPH solution is linearly related to its absorbance at 517nm in the range of 0.01mM~0.08mM, and its linear regression equation is: A=0.1337c(DPPH)-0.0091, R 2 = 0.9981.

[0036] Take 0.1mL of sample solutions of different concentrations, add 1.9mL of 0.065mM DPPH solution and mix well. React at room temperature for a certain period of time, and measure the absorbance A at 517nm i . The corresponding solvent was used instead of the sample as a blank control, and the absorbance was A max , the absorbance of the corresponding sample solution is A 0 . Three parallel samples were made for each sample, and the average value was taken. The scavenging activity of DPPH free radicals is calculated acco...

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Abstract

The invention discloses an extract of agrimony total flavonoids and an extraction and preparation method thereof; the total amount of ketosteroid compounds accounts for 25 percent to 100 percent of the total extraction matter. The invention takes agrimony as raw material and organic solvent whether containing or not containing water as extraction solvent. The extraction procedure is as following: extracting, leaching with solvent and adsorbing resin column to make purification process or using inorganic acid to regulate the PH value of concentrated solution to be no higher than 3, and then obtaining the extraction matter of agrimony after processing. Experiments prove that the extraction matter of agrimony total flavonoids has functions of antioxidation, anti-myocardial ischemia and reducing blood lipid. The extraction matter of agrimony total flavonoids can be used as food preservative and health product additive; the extraction matter of agrimony total flavonoids, carriers acceptable in pharmacy or excipient are made into various pharmaceutical dosage forms for anti-myocardial ischemia and reducing blood lipid. The invention has the advantages of simple technics and good treatment effect and can be widely used for curing or preventing coronary heart disease, hyperlipoidemia, high blood pressure or preventing cardio-cerebrovascular diseases such as atherosclerosis, etc.

Description

Technical field: [0001] The invention relates to Agrimony total flavonoids extract, a preparation method thereof, and the use of the extract as an antioxidant and in the preparation of cardiovascular and cerebrovascular medicines. Background technique: [0002] Agrimonia pilosa, also known as deli grass, melon herb, old beef tendon, wolf bud grass, is the aerial part of the perennial herbaceous plant Agrimonia pilosa Ledeb. in the family Rosaceae. Born in hillside forests, roadsides and ditches. Mainly produced in Zhejiang, Jiangsu and Hubei. In traditional Chinese medicine, the main medicinal effects are astringent to stop bleeding, stop malaria, stop dysentery, and detoxify. For hemoptysis, hematemesis, malaria, fatigue, carbuncle. [0003] There are many research reports on the pharmacological effects of Agrimony water extract. Modern research shows that the water extract of Agrimony has cardiotonic, antihypertensive, hypoglycemic, antibacterial, insecticidal and anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61K9/02A61K9/06A61K9/10A61K9/14A61K9/20A61K9/48A61P9/10
Inventor 祝连彩王伯初谭君贺蕊余正文隋婧
Owner CHONGQING UNIV
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