Method for detecting drugs in human bodies

A detection method and drug technology, applied in the field of drug detection in the human body, can solve the problems of high false positives, few types, and low detection result accuracy, and achieve high accuracy, low detection limit and quantification limit, and convenient material sampling. Effect

Inactive Publication Date: 2019-09-27
BGI FORENSIC TECH (SHENZHEN) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, most of the drug detection kits are single-item or the same type of drug detection kits, with fewer types
Moreover, there are disadvantages of low accuracy and high false positives in the detection results.

Method used

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  • Method for detecting drugs in human bodies
  • Method for detecting drugs in human bodies
  • Method for detecting drugs in human bodies

Examples

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Effect test

Embodiment 1

[0073] A method for detecting drugs in a human body, wherein the drugs to be detected include 14 kinds, specifically amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine, ketamine, heroin, morphine, codeine, piperazine Tidine, methadone, fentanyl, 6-monoacetylmorphine, tetrahydrocannabinol, benzoylecgonine, cocaine, the detection method includes:

[0074] Collect 5 mL of saliva from 5 normal adults, mix well and centrifuge for 5 minutes, take the supernatant, add 10 mL of deionized water, vortex for 15 s, then add 30 mL of precipitant, vortex for 15 s, and centrifuge at 3000 rpm for 1 min at room temperature. After taking the supernatant, add the mixed standard of drugs, so that the final concentration of each drug added is 10ppb, and the obtained mixed sample is used as the sample to be tested, simulating the real sample, and take 100 μL of the mixed sample and add it to the injection vial for later use.

[0075] Take 14 drug standard substances, dilute them step b...

Embodiment 2

[0094] A method for detecting drugs in a human body, wherein the drug is heroin, and the method for detecting comprises:

[0095] Sample processing: Collect 6 mL of saliva from 6 normal adults at 5 p.m., mix well and centrifuge for 5 minutes, take the supernatant, add 12 mL of deionized water, vortex for 15 s, then add 18 mL of precipitant, vortex for 15 s , centrifuge at room temperature at 3000rpm for 5min, take the supernatant and add the standard substance of heroin, so that the theoretical final concentration of heroin added is 10ppb, and the mixed sample obtained simulates the real sample, and 150 μL of the mixed sample is added to the injection vial for subsequent use.

[0096] Take 14 drug standard substances, dilute them step by step with deionized water, and prepare standard solutions of 0.1ng / mL, 0.5ng / mL, 1.0ng / mL, 10ng / mL, 50ng / mL, and 100ng / mL, and then The standard solutions of corresponding concentrations of 14 kinds of drugs were mixed respectively, so as to p...

Embodiment 3

[0110] A detection method for narcotics in a human body, the detection method comprising:

[0111] Sample processing: Collect 2 mL of saliva from a drug addict at 6 p.m., mix well and centrifuge for 3 minutes, take the supernatant, add 4 mL of deionized water, vortex for 15 s, then add 6 mL of precipitant, vortex for 15 s, Centrifuge at 3000 rpm for 3 minutes at room temperature, take 1 mL of the supernatant for use, and take 150 μL of the sample and add it to the injection vial for testing; when collecting saliva samples, draw 5 mL of venous blood at the same time for use.

[0112] Take 14 drug standard substances, dilute them step by step with deionized water, and prepare standard solutions of 0.1ng / mL, 0.5ng / mL, 1.0ng / mL, 10ng / mL, 50ng / mL, and 100ng / mL, and then The standard solutions of corresponding concentrations of 14 kinds of drugs were mixed respectively, so as to prepare 6 kinds of standard substance mixed solutions of concentrations, and set aside.

[0113] Using l...

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Abstract

The invention provides a method for detecting drugs in human bodies. The method comprises the following steps of: preparing a series of mixed drug standard solutions with gradient concentrations by using 14 drug standards; respectively performing mass spectrum detection on a sample and the mixed drug standard solutions by adopting a liquid chromatography-tandem mass spectrometry (LC-MS/MS), and determining one or more than one drugs in the mixed drug standard solutions in the sample on the basis of a mass spectrum detection result so as to achieve qualitative analysis; drawing a standard curve, detecting the peak area of a parent ion/child ion pair corresponding to at least one of the 14 drugs in the sample, and obtaining the concentration of the sample according to the standard curve for quantitative analysis, wherein the sample is optionally derived from human saliva, and optionally, the sample is pretreated. The method for detecting drugs in human bodies has the advantages of convenient material obtaining, the small dosage, the low detection limit and quantitative limit, the high accuracy, the high efficiency and the like.

Description

technical field [0001] The invention relates to the technical field of biomedicine detection, in particular to a method for detecting drugs in human body. Background technique [0002] Drugs are becoming more and more harmful to human life, and drug smuggling and drug abuse are becoming a rapidly growing and increasingly serious social problem. The drugs taken by drug addicts mainly include heroin, morphine, methamphetamine, ketamine, marijuana and ecstasy, etc. In view of the serious harm to the lives of themselves and the people around them, it is urgent to establish an accurate, fast and efficient detection method for drugs in vivo . [0003] Saliva is a digestive juice mixed with mucus secreted by the sublingual, mandibular, costal, and many small mucous glands on the walls of the mouth. In recent years, through the research on saliva, it has been found that saliva has many new uses. After entering the human body, drugs are quickly absorbed and further metabolized. Fr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88
Inventor 李生斌王轶男饶维桥廖云莉陈跃华王泳钦卢鹏飞
Owner BGI FORENSIC TECH (SHENZHEN) CO LTD
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