High performance liquid chromatography detection method for anthocyanin in blueberries

A high-performance liquid chromatography and detection method technology, applied in the field of photoelectrode materials, can solve problems such as affecting analysis results and difficult separation, and achieve the effects of simple and rapid operation, optimized separation conditions, and low quantitative limit

Active Publication Date: 2021-06-11
GUIZHOU INST OF BIOLOGY
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  • Abstract
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  • Claims
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Problems solved by technology

At present, the prior art discloses the simultaneous determination of six basic anthocyanins in grapes and wine by using high performance liquid chromatography (see "Simultaneous determination of six basic anthocyanins in grapes and wine by HPLC", Liu Bing et al., China Brewing, 2017,36(002):162~165), but only the anthocyanins in grapes and wines containing less anthocyanins were well separated and analyzed, while for blueberries containing more anthocyanins (containing at least 13 Anthocyanins), it is difficult to completely separate the six basic anthocyanins, which will inevitably affect the analysis results

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  • High performance liquid chromatography detection method for anthocyanin in blueberries
  • High performance liquid chromatography detection method for anthocyanin in blueberries
  • High performance liquid chromatography detection method for anthocyanin in blueberries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] 1. Materials and reagents: The blueberries tested were collected from the blueberry planting base in Majiang County, Kaili City, Guizhou Province, and the blueberry variety is the rabbit eye series. Reagents include chromatographically pure acetonitrile, methanol, analytically pure formic acid, acetic acid, phosphoric acid, ethanol, citric acid, ethyl acetate

[0105] 2. Instruments and equipment: high performance liquid chromatography system (Agilent1260 liquid chromatograph, equipped with 1260 diode array detector, American Agilent Company); LGJ-50F vacuum freeze dryer (Beijing Songyuan Huaxing Technology Development Co., Ltd.); BEATL-10 Ultrapure water preparation instrument (Shandong Tinglan Environmental Protection Technology Co., Ltd.); in addition, it also involves rotary evaporator, ultrasonic cleaning instrument, digital display constant temperature water bath, and ultrafiltration device.

[0106] Three, the preparation of blueberry anthocyanin extract:

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Embodiment 2

[0144] Take 10mg of blueberry anthocyanin powder, dissolve it with 30wt% methanol solution and dilute to 10mL to obtain a sample solution, filter the sample solution with a 0.22 μm organic filter membrane to obtain a test solution for later use.

[0145] The liquid to be tested is detected by the same high-performance liquid chromatography detection conditions as in Example 1, and the high-performance liquid chromatography spectrum obtained figure 2 , figure 2 1 is delphinidin-3-O-glucoside, 2 is cyanidin-3-O-glucoside, 3 is petunia-3-O-glucoside, 4 is pelargonin-3 -O-glucoside, 5 is paeoniflorin-3-O-glucoside, and 6 is mallow pigment-3-O-glucoside.

[0146] Calculate the content of 6 main anthocyanins in the blueberry anthocyanin powder according to the standard equation provided in Example 1, the results are shown in Table 8, by figure 2 As can be seen from Table 8, Example 2 has achieved the determination of 6 main anthocyanins in blueberry anthocyanin powder through o...

Embodiment 3

[0150] Take an appropriate amount of blueberry red wine and filter it with a 0.22 μm organic filter to obtain the test solution for later use.

[0151] Using the same high-performance liquid chromatography detection conditions as in Example 1, 60 μL of the liquid to be tested was injected for detection, and the obtained high-performance liquid chromatography image 3 , image 3 1 is delphinidin-3-O-glucoside, 2 is cyanidin-3-O-glucoside, 3 is petunia-3-O-glucoside, 4 is pelargonin-3 -O-glucoside, 5 is paeoniflorin-3-O-glucoside, and 6 is mallow pigment-3-O-glucoside.

[0152] Calculate the content of 6 main anthocyanins in blueberry red wine according to the standard equation that embodiment 1 provides, and the results are shown in table 3, by image 3 As can be seen from Table 9, Example 3 achieves the determination of five main anthocyanins in blueberry red wine through one detection. It can be seen from Table 3 that the content of mallow pigment-3-O-glucoside in blueberry ...

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Abstract

The invention provides a high performance liquid chromatography detection method for anthocyanin in blueberries, and belongs to the technical field of photoelectrode materials. The anthocyanin comprises delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, pelargonidin-3-O-glucoside, paeoniflorin 3-O-glucoside and malvidin 3-O-glucoside, wherein the flow rate of the high performance liquid chromatography is 0.8 mL / min, the detection wavelength is 520 nm, the detection temperature is 25 DEG C, the sample injection volume is 10 microliters, the detection time is 60 minutes, mobile phases: an aqueous phase B is a 0.3 wt% phosphoric acid aqueous solution, and an organic phase A is acetonitrile, and gradient elution. According to the present invention, the simultaneous determination of the six anthocyanins in the blueberry sample is achieved, the standard deviation of the peak area and the retention time is small, the detection limit and the quantification limit of the six anthocyanins are low, and the method has characteristics of high sensitivity, good precision, good reproducibility and good stability.

Description

technical field [0001] The invention relates to the technical field of photoelectrode materials, in particular to a high performance liquid chromatography detection method for anthocyanins in blueberries. Background technique [0002] Anthocyanins or anthocyanins are water-soluble natural pigments, the basic structure of which is 2-phenylbenzopyran, namely the Flavylium, and its structure is shown in the following formula. There are more than 500 kinds of anthocyanins known in nature, of which 6 are the most common, as shown in Table 1, namely cyanidin or hibiscus anthocyanin (Cyanidin), delphinidin or delphinidin , Delphinidin, Peonidin, Petunia or Petunidin, Malvidin and Pelargonidin. Anthocyanins generally exist in the form of glycosides, and often form anthocyanins or anthocyanins (Anthocyanin) through glycosidic bonds with one or more glucose, rhamnose, galactose, arabinose, etc., in which anthocyanin-3-O- Glucoside is the main anthocyanin. [0003] [0004] Table...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8634G01N2030/047
Inventor 徐青周元敬龙尚俊聂飞颜昌锐杨江周友松
Owner GUIZHOU INST OF BIOLOGY
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