Nucleic acid aptamer fluorescence test strip, method for preparing same and application of nucleic acid aptamer fluorescence test strip

Abstract

The invention provides a nucleic acid aptamer fluorescence test strip, a method for preparing the same and application of the nucleic acid aptamer fluorescence test strip, and relates to the technicalfield of biological detection. The nucleic acid aptamer fluorescence test strip comprises a bottom plate, a sample pad, a nanometer material pad, a reaction film and a water absorption pad. The reaction film is provided with a detection line, and the detection line is arranged at the end, which is close to the nanometer material pad, of the reaction film; nucleic acid aptamer with fluorescein labels and / or nucleic acid aptamer with fluorescence labels and linker sequences and free fluorescein are attached to the nanometer material pad, and complementary strains of nucleic acid aptamer or complementary strains of linker sequences of nucleic acid aptamer are fixed to the detection line. The method for preparing the nucleic acid aptamer fluorescence test strip includes sequentially adheringthe sample pad, the nanometer material pad, the reaction film and the water absorption pad on the same side of the bottom plate. The nucleic acid aptamer fluorescence test strip, the method and the application have the advantages that the nucleic acid aptamer fluorescence test strip is simple in structure, sensitive in detection and high in accuracy; the method is easy to implement and high in flexibility.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a nucleic acid aptamer fluorescent test strip and its preparation method and application. Background technique [0002] As a simple and fast analytical tool, test strips have been widely used in clinical diagnosis, food safety and environmental monitoring. At present, there are mainly two detection methods of colloidal gold and fluorescence test strips. Among them, the colloidal gold test strips display the results through color reaction, which has the advantage of being visible to the naked eye without the need for other instruments; but its disadvantage is low sensitivity, and can only be tested by comparison The color depth achieves the purpose of semi-quantitative. Compared with colloidal gold test strips, fluorescent test strips are highly sensitive, and can be used with a portable fluorescence detector to accurately quantify the target in the sample, so it has...

AI Technical Summary

Problems solved by technology

However, there are not many colloidal gold or fluorescent test strips based on nucleic acid aptamers. The main reason is that different aptamer sequences have different lengths. It is greater than the affinity of the complex formed by the aptamer and the target, so the aptamer that has bound to the target still dissociates at the detection line, and the accurate detection of the target cannot be achieved

Although the fixed antigen on the detection line makes the competitiveness consistent, for many small molecule targets, the combination with the coupling protein limits the binding to the aptamer, making the aptamer unable to bind at the detection line, and cannot achieve sensitive and accurate detection

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