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VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus, and preparation and applications thereof

A vaccinia virus, VTT-VGFR technology, applied in the direction of virus, viral peptide, genetic engineering, etc., can solve the problem of lack of attenuated Tiantan strain virus with VGF gene removed, and achieve the effect of improving tumor selectivity and reducing toxicity

Pending Publication Date: 2019-09-06
西安彤盛生物科技有限公司
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, there is still a lack of attenuated Tiantan strain viruses that have removed the VGF gene.

Method used

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  • VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus, and preparation and applications thereof
  • VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus, and preparation and applications thereof
  • VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus, and preparation and applications thereof

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Embodiment Construction

[0038] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0039] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0040] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0041] The DNA sequence of the wild vaccinia virus Tiantan strain in the present invention is the sequence of GenBank No. AF095689.1 (VRL14-FEB-2000).

[0042] The wild-type vaccinia virus Tiantan strain in the following examples is preserved in the Laboratory of Molecular Virology and Viral Immunology of Xi'an Medical College (this laboratory, the same below). The pMV-VTTVGFE plasmid was preserved by our laboratory; the cell lines CV-1, A549...

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Abstract

The invention discloses a VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus. A Tiantan strain oncolytic vaccinia virus VGF gene is removed, an EGFP gene is expressed, and the base sequence of the VGF gene is shown as a sequence table 1. A preparation method and applications of the VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus are also disclosed. Through the VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus VTT-VGFE, the toxicity of the Tiantan strain oncolytic vaccinia virus can be reduced, and the tumor selectivity of the Tiantan strain oncolytic vaccinia virus can be enhanced; and the VGF gene removed recombinant Tiantan strain oncolytic vaccinia virus VTT-VGFE has a function of monitoring the distribution of virions in vivo inreal time, provides a good tool for researching and developing live vaccines and tumor biotherapy by taking vaccinia virus as a carrier, and can be suitable for treating lung cancer, liver cancer, melanoma and other various tumors.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, and relates to a recombinant Tiantan strain oncolytic vaccinia virus with VGF gene removed, and also relates to a preparation method and application of the recombinant Tiantan strain oncolytic vaccinia virus with VGF gene removed. Background technique [0002] Tumor biotherapy technology is a brand-new tumor treatment method that has emerged in recent years. It has the characteristics of small side effects, high anti-tumor activity, and wide adaptability. [0003] As an oncolytic virus, vaccinia virus (VACV) has natural tropism for tumors, can selectively infect tumor cells and replicate in them, and will not be quickly cleared by the immune system. Vaccinia virus Tiantan strain (TianTan strain, VTT) is a unique strain of vaccinia virus in my country. As a human vaccine against smallpox, it has been widely used all over the world. Therefore, vaccinia virus provides a new direction ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/63C12N15/90C12N15/65A61P35/00C12R1/93
CPCC07K14/005C12N15/63C12N15/902C12N15/65A61P35/00C12N2710/24021C12N2710/24022C12N2710/24032C12N2710/24034
Inventor 马茜汪洋
Owner 西安彤盛生物科技有限公司
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