Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of atractylodin alcohol in medicament preparation

A technology of atractylodes alcohol and drugs, applied in the direction of active ingredients of heterocyclic compounds, antiviral agents, etc., can solve the problems of no effect and its mechanism, reports, etc., and achieve the effect of significant curative effect, small side effects and moderate dosage

Inactive Publication Date: 2012-02-15
YUNNAN AGRICULTURAL UNIVERSITY +1
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The small molecule compound atractylodinol (CZSC) is derived from the Atractylodes atractylodes plant, and there is no report on the anti-PRRSV infection effect and mechanism of CZSC in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of atractylodin alcohol in medicament preparation
  • Application of atractylodin alcohol in medicament preparation
  • Application of atractylodin alcohol in medicament preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The compound monomer atractylodinol CZSC was serially diluted 2-fold with DMEM culture medium, and serially diluted into 5 gradients; each gradient was inoculated into 3 cell wells, and the total amount of the mixed solution was 100 μl, which was inoculated into a 96-well cell plate (Corning Inc. ) in a monolayer of Marc-145 cells, cultured in a carbon dioxide incubator at 37°C for 3 days, and then used the cell viability detection kit CCK-8 to measure the cell viability: add 10 μl 2-(2-formazol to each well of cells Oxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid phenyl)-2H-tetrazole monosodium salt (WST-8) reagent, incubated 4 hours, then measure its absorbance value (OD450) at 450nm with a microplate reader, according to the OD value, use the Reed-Much method to calculate the half-maximum toxic concentration TC of CZSC to cells 50 (50% toxic concentration) and the maximum non-toxic dose concentration TC 0 (0% toxic concentration) 358.9μM and 157.6μM resp...

Embodiment 2

[0030] The atractyloid alcohol CZSC was made 2-fold serial dilution respectively, and then different concentrations of drugs were mixed with 300 TCID 50 The virus liquid was mixed, and then the virus drug mixture was inoculated into 96-well cell plates (Corning Company) covered with monolayer Marc-145 cells, and a normal cell control group (without drug and virus) and a virus control group (only Contain virus), continue culturing in 37°C, 5% CO2 incubator, observe the cell CPE (cytopathic effect) day by day, the CPE of PRRSV-induced Marc-145 cells is mainly manifested as: cells shrink, aggregate, and finally fall off; culture observation 3 Days later, the following conclusions were drawn according to the differences in CPE changes in different treatment groups: CZSC could inhibit the proliferation of PRRS virus in Marc-145 cells in the concentration range of 19.7-157.6 μM, significantly reduce the damage effect (CPE) of the virus on cells, and inhibit The effect was dose-depen...

Embodiment 3

[0032] The real-time fluorescent PCR method was used to detect the effect of drugs on the expression of PRRSV ORF7 gene mRNA. The total RNA of the drug intervention group and the virus control group was extracted using RNAiso TMPlus reagent, referring to the instructions. After reverse transcription of the extracted total RNA, use iQ5real time A PCR instrument (Bio-Rad) was used to amplify the PRRSV ORF7 gene. According to the internal reference gene β-action gene and the CT value of the target gene obtained by fluorescence quantification, the expression ratio of the ORF7 gene in the drug intervention experimental group relative to the virus control group was calculated by the Pfaffl method . Experimental results such as image 3Shown: compound CZSC is in the concentration range of 19.7~157.6 μ M, and the expression of ORF7 mRNA of PRRS virus in the drug intervention group is significantly lower than that of the virus control group (P<0.005), suggesting that the compound can i...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of an atractylodin alcohol (CZSC) compound in preparation of medicaments for treating animal virus diseases, in particular application in preparation of a medicament for treating porcine reproductive and respiratory syndrome virus (PRRSV) infection and a medicament for treating porcine reproductive and respiratory syndrome (PRRS), or in preparation of a virus gene expression inhibitor and an anti-virus medicament and in preparation of a medicament for inhibiting blue ear disease viruses. The CZSC has remarkable effects of regulating nucleoprotein gene expression of the PRRSV down and inhibiting virus proliferation; and in vitro cellular pharmacological tests shows that the CZSC has definite anti-virus effect. The CZSC has wide application prospect in treatment and control of PRRSV infection.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to the application of atractyloid alcohol (CZSC) in the preparation of medicines for treating viral diseases in animals, especially the application in the preparation of medicines for treating porcine reproductive and respiratory syndrome virus infection, and in the preparation of medicines for treating porcine reproductive and respiratory syndrome. Syndrome drugs, or in the preparation of viral gene expression inhibitors, in the preparation of antiviral drugs, and in the application of drugs for inhibiting PRRS virus. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), also known as blue ear disease, is one of the important infectious diseases that cause huge economic losses to the pig industry worldwide. In 1987, the existence of PRRS was first reported in the United States, which brought economic losses of up to 560 million U.S. dolla...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/34A61P31/14
Inventor 尹革芬程永现毕峻龙吕青杨贵树李想
Owner YUNNAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com