22 results about "Viral isolation" patented technology

The invention relates to the technical field of biological detection, and discloses a composition and a kit for detecting a virus of positive bunyavirus and application of the composition and the kit. The composition disclosed by the invention can be used for rapidly and simultaneously detecting the oropercut virus, the Batai virus and the Akabai virus in a sample in a high-specificity manner, and has relatively high sensitivity and stability. Compared with traditional detection methods such as virus separation, genome sequencing and the like, the efficiency of identifying and detecting the oropercut virus, the Batai virus and the Acamovirus by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

This application discloses a circular RNA vaccine against porcine reproductive and respiratory syndrome (PRRS) virus, its construction method, and its application. The circular RNA vaccine comprises circular RNA and a pharmaceutically acceptable carrier. The coding elements in the circular RNA encode the GP4, GP2, GP5, and M proteins of PRRS virus. The circular RNA vaccine exhibits high stability and good safety, producing no adverse reactions after immunization of pigs. It induces high levels of neutralizing antibodies in pigs after immunization and activates a high level of cellular immune response, providing up to 100% immunoprotection. Furthermore, its preparation process eliminates the need for virus isolation, significantly shortening the research and development cycle. The production process is not constrained by biological factors such as virus titer, facilitating standardization and large-scale production, and effectively reducing production costs.

The invention relates to the technical field of water plant virus separation, and discloses a water plant virus separation method and application thereof, and the method comprises the step of mixing an amino-functionalized carbon nanomaterial and a water body containing plant viruses. The amino-functionalized carbon nanomaterial adopted by the method can be combined with plant viruses in a water body, so that the adsorption capacity of the amino-functionalized carbon nanomaterial to the plant viruses is improved, and the method is simple to operate, efficient and low in cost, does not generate toxic substances, avoids secondary pollution possibly caused by chemical disinfection, and is suitable for industrial production. The invention provides an economic and sustainable solution for solving the problem of plant virus pollution, and shows a wide application prospect in the field of water plant virus removal.

The invention discloses a virus separation and enrichment micro-fluidic device based on an ATPS technology. The virus separation and enrichment micro-fluidic device comprises a micro-fluidic chip, a pump valve system and a temperature control module, and a sample pretreatment area, an ATPS mixed reaction area, a rapid phase separation area and a target phase collection area are sequentially integrated in the micro-fluidic chip; the ATPS mixed reaction area is of a snakelike micro-channel structure, the rapid phase separation area is of a wide and shallow type sedimentation chamber structure, the temperature control module comprises a temperature control plate and a temperature sensor, the temperature control plate is attached to the bottom surface of the micro-fluidic chip, and the temperature sensor is attached to the bottom surface of the micro-fluidic chip and is correspondingly located at the center position of the rapid phase separation area. According to the present invention, the high-purity virus enrichment liquid can be separated from the complex environmental sample, the advantages of full automation, sealing, high efficiency and no manual intervention are provided, the bottlenecks of the traditional ATPS technology in the aspects of speed, automation and field applicability are broken through, and the reliable and efficient front-end separation solution is provided for the rapid virus detection.

The present application relates to the technical field of aquatic plant virus separation, and discloses a method for separating aquatic plant viruses and application thereof, wherein the method comprises mixing amino-functionalized carbon nanomaterials and aquatic bodies containing plant viruses. The amino-functionalized carbon nanomaterials used in the method can be combined with the plant viruses in the aquatic bodies, thereby improving the adsorption capacity of the amino-functionalized carbon nanomaterials for the plant viruses. The method is simple, efficient and low in cost, and does not produce toxic substances, thereby avoiding secondary pollution possibly caused by chemical disinfection, providing an economic and sustainable solution to the problem of plant virus pollution, and showing a broad application prospect in the field of aquatic plant virus removal.

The invention belongs to the technical field of virus inactivation equipment, and particularly relates to a virus inactivation device for artificial biological tendon preparation, which comprises a normal saline cylinder, the lower surface of the normal saline cylinder is fixedly connected with a plurality of support legs, and the outer walls of the plurality of support legs are jointly and fixedly connected with a bottom plate; the lower surface of the bottom plate is fixedly connected with a driving motor and a PLC. The virus inactivation device for preparing the artificial biological tendon has the functions of virus separation and inactivation, provenance cells and a biological scaffold material are prevented from being damaged in the virus inactivation process, the activity and the function of the provenance cells are guaranteed, and the adverse effect of the performance of the biological scaffold material is avoided; and the device has an efficient protection function through the heat preservation mechanism, so that the preparation quality of the artificial biological tendon is effectively improved, meanwhile, no accurate control step and no complex instrument are used in the virus inactivation process, time and labor are saved, and the preparation convenience and efficiency of the artificial biological tendon are effectively improved.

The invention relates to the technical field of virus isolated culture, in particular to a method for promoting avian leukosis virus proliferation. The invention provides a method for promoting proliferation of avian leukosis viruses. The method comprises the step of culturing the avian leukosis viruses by taking STAT1 gene inactivated chicken DF-1 cells as host cells. The DF-1 cell strain with the STAT1 knocked out is established, when the cell strain is used for isolated culture of ALV, the proliferation rate of the ALV can be effectively increased, compared with an original DF-1 cell, the proliferation process of the virus can be remarkably accelerated, and the proliferation time is shortened from at least 7 days to 4 days. The DF-1 cell strain of which the STAT1 is knocked out can be used as a host cell to be applied to separation, amplification or detection of ALV in the industry, and shows an ideal amplification effect for poultry plasma samples under different health conditions.

Here, the inventors report the biochemical and functional characterization of a new PTM on a key ESCRT-III member and its impact during viral budding. They discovered that CHMP2B K6 plays a pivotal role in both abscission and HIV viral budding, highlighting the importance of the novel methylation event. Strikingly, expression of unmethylatable CHMP2B mutants (CHMP2B K6A, CHMP2B K6R) perturbed the localization of ESCRT-III at the ICB with delayed recruitment and distribution of CHMP2B to the cleavage site, ultimately leading to impeded abscission. They reported that expression of CHMP2B mutant bearing a specific lysine point mutation at position 6 drastically reduces the number of viral particles released into the supernatant. Additionally, this mutation impairs the infectivity of the produced virions. This is the first description of the antiviral effect of a point mutant of ESCRT-III proteins. Thus, the present invention relates to an isolated polypeptide derived from unmethylatable mutant CHMP2B and its uses for treating infection.

The swab collection assembly comprises a swab and a collection tube used for containing the swab, the swab comprises a rod handle and a collection cotton head which are connected, an easy-to-break notch is formed in the middle section of the rod handle, a baffle is arranged at an opening of the collection tube, and the collection cotton head is connected with the rod handle. The center of the baffle is provided with a one-way channel forming an included angle with the axis of the collecting tube, the one-way channel comprises a notch section located on the opening side and a guide section located below the notch section, the bottom of the guide section is connected with one end of an elastic plate, the narrowest position of the one-way channel is matched with the outer diameter of the swab, and the elastic plate is connected with the other end of the elastic plate. The surface of the baffle is covered with a sealing film. The collecting tube with the cover can be prevented from being unscrewed and closed, carried-out virus separation liquid is prevented from splashing, the collecting process is smoother, operation is easier and more convenient, the working efficiency of medical workers is effectively improved, and meanwhile the medical workers are prevented from being infected.

Provided is a method of enriching full adenovirus-associated virus (AAV) capsids from a mixture of full AAV capsids and empty AAV capsids, the method comprising providing a sample comprising a mixture of full AAV capsids and empty AAV capsids, subjecting the sample to anion exchange chromatography comprising an elution buffer comprising an equilibration buffer and a salt-containing buffer in an initial ratio that provides an initial conductivity, and changing the ratio of the equilibration buffer and the salt-containing buffer to provide a step gradient conductivity increase of about 0.5-2.0 mS / cm in each step to elute empty AAV capsids and to provide a fluid enriched with full AAV capsids.

The invention discloses a method for separating porcine reproductive and respiratory syndrome virus and porcine circovirus, and belongs to the technical field of virus separation. The separation method comprises the following steps: preparing a virus positive sample into homogenate, and centrifuging to obtain virus liquid; mixing the virus solution with polypropylene glycol and a cholesterol solution to obtain a virus infection solution; inoculating cells with the virus infection solution, and co-culturing in a virus culture solution; and after co-culture is finished, freezing and thawing the cell culture for three times to serve as an F1 generation, subculturing to an F3 generation according to the steps (1)-(3), and harvesting venom. According to the method, polypropylene glycol and cholesterol are added in the virus separation process, the sensitivity of cells to viruses is improved, noradrenaline is added into a virus culture solution, a cell signal channel is adjusted, virus proliferation and release can be promoted, and the purpose of rapidly separating the viruses is achieved.

Proposed are a foot-and-mouth disease vaccine composition including recombinant foot-and-mouth disease viruses and an antigen isolated and purified from the viruses. In the early stages of vaccination, a humoral immune response is simultaneously induced through the induction of a robust cellular immune response, while in the presence of maternally-derived antibodies (MDAs), B cell receptors are stimulated. Through this, it is possible to provide the vaccine composition that enables active immunity and overcomes the interference of the maternally-derived antibodies and to provide a method of preventing or treating foot-and-mouth diseases using the same composition.

This invention relates to the field of virus isolation and purification technology, and discloses a method for efficiently enriching viruses using polyglutamic acid in synergistic effect with calcium ions. The method includes: preparing a polyglutamic acid mother liquor, a calcium ion mother liquor, and a chelating agent working solution; adding the polyglutamic acid mother liquor and the calcium ion mother liquor sequentially to the virus lysis buffer and adjusting the pH to obtain a mixed solution; centrifuging after static sedimentation to obtain a wet precipitate; dissolving the wet precipitate in the chelating agent working solution, diluting with PBS buffer, purifying by ultrafiltration, washing, and collecting the retentate; and adding sucrose and bovine serum albumin to obtain the enriched virus solution. This invention, by sequentially adding high molecular weight polyglutamic acid mother liquor and calcium ion mother liquor to the virus lysis buffer, utilizes the coordination cross-linking effect formed between calcium ions and polyglutamic acid on the virus surface to capture dispersed virus particles and form flocculated precipitates, thereby shortening the sedimentation time and improving the virus recovery rate.

The application discloses a micro-fluidic cell chip and a virus separation and culture method based on the cell chip. The micro-fluidic cell chip comprises a metal frame, a lower cover plate, a chip frame, a core chip and an upper cover plate, the core chip is provided with a plurality of cell culture cavities, a main flow channel is arranged on the surface of the core chip, and the main flow channel is communicated with the plurality of cell culture cavities. The virus separation and culture method based on the micro-fluidic cell chip adopts a plurality of cell lines for co-culture, realizes the sequential incubation of one sample to be analyzed and different cell lines, can significantly save clinical samples, maximally improves sample utilization, simultaneously reduces the consumption of culture reagents, shortens the separation and identification time, and theoretically realizes the culture of at least ten different cell lines at the same time due to the design of the micro-fluidic cell chip provided by the application, significantly increases the throughput of virus sensitive host cell screening, and realizes the high-throughput, automatic and rapid screening of virus sensitive cell lines.

There is provided a method of separating a smaller virus from a larger virus from a fluid, the method comprising (a) passing the fluid containing the smaller virus and the larger virus through a porous filter comprising a porous medium and obtaining the smaller virus at a differential pressure of at least 20 psid; (b) reducing the pressure to 3 psid or less; and (c) passing an additional virus-containing fluid through the porous filter comprising the porous medium at a pressure of at least 20 psid and obtaining an additional smaller virus.

This invention discloses a method for efficiently isolating human parainfluenza virus based on suspended MDCK cells. By using the fully suspended passaged MDCK cell line for human parainfluenza virus isolation and culture and optimizing the inoculation process, single-generation virus amplification can be completed within 3 days, significantly shortening the cycle, improving virus isolation efficiency and success rate compared to traditional adherent culture methods, and enhancing experimental reproducibility. This method not only demonstrates good isolation and culture capabilities for the major subtypes of human parainfluenza virus, but also shows good adaptability to clinical samples from different sources and with different initial loads. In addition, this culture system has linear scale-up potential, is not limited by the surface area of ​​adherent culture, and can meet the large-scale virus seed preparation needs from clinical testing to vaccine mass production, providing key technical support for the integrated process from human parainfluenza virus isolation to vaccine production.

The invention relates to the technical field of filtering materials, in particular to a positively charged ceramic membrane and a preparation method and application thereof.The preparation method comprises the steps that two kinds of Al2O3 particles with different particle sizes are taken and dispersed in ZrO2 or TiO2 sol, two kinds of precursor solutions are obtained, a tubular supporting body is sequentially coated with the two kinds of precursor solutions, drying and sintering are conducted, and the positively charged ceramic membrane is obtained; repeatedly coating, drying and sintering to obtain a tubular ceramic membrane; nano MgO particles with the particle size of 0.03-0.1 [mu] m are uniformly dispersed in the second sol, the content of nano MgO is 1-5%, and a sol charging agent is obtained; and immersing the tubular ceramic membrane in the sol charging agent for 3-5 min, and then carrying out third drying and third sintering to obtain the positively charged ceramic membrane. The positively charged ceramic membrane disclosed by the invention has the advantages of good virus interception effect, high flux, low cost, simple preparation process and strong pollution resistance, and can be widely applied to a virus separation process in water treatment.

The utility model discloses a virus separating device with a multi-layer filtering function, which comprises a base and a clamping component arranged on the side edge of the base, and the clamping component is used for ensuring the stability of a needle cylinder; the device further comprises a pressure regulating assembly arranged on the base, and the pressure regulating assembly is used for reducing the manual workload. The utility model belongs to the technical field of medical instruments, and particularly relates to a virus separation device with a multi-layer filtering function.

The invention discloses construction of an antibody-enhanced monkey pox virus infection target cell model based on gene modification. Aiming at the problems that monkey pox virus in-vitro infection target cells are few in types and high virus titer (especially IMV) is needed, the nano antibody which is high in affinity and is combined with D8L protein on the surface of IMV and does not block infection is prepared; constructing a cell surface expression vector containing the antibody gene, and transfecting VERO cells or epidermal cells to obtain a stable expression cell line; the cell line can specifically enrich trace IMV in a sample, obviously improve the infection efficiency, reduce the effective infection virus titer of VERO cells by more than 100 times, and realize effective infection of IMV on epidermal cells. The method solves the problem of difficulty in IMV separation in a clinical low-titer sample, can be used in the fields of IMV-related virus separation, mechanism research, drug screening and the like, and has important scientific research and clinical values.

A method for separating smaller viruses from larger viruses from a fluid is provided, comprising the steps of: (a) passing a fluid containing the smaller viruses and the larger viruses through a porous filter comprising a porous medium at a differential pressure of at least 20 psid to obtain the smaller viruses; (b) reducing the pressure to 3 psid or less; and (c) passing additional fluid containing the viruses through the porous filter comprising the porous medium at a pressure of at least 20 psid to obtain additional smaller viruses.

The invention discloses separation, identification and application of a porcine bocavirus cell adapted strain, and belongs to the technical field of gene engineering. The preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46413, and the strain can be stably proliferated in passage cells of porcine kidney cells (PK) and can induce a remarkable cytopathic effect. Virus separation and identification are completed through metagenome sequencing, transmission electron microscope observation and indirect immunofluorescence technologies, and a phylogenetic tree is constructed based on a VP1 gene sequence. The invention also establishes a piglet infection model, and proves that the porcine bocavirus cell adapted strain CNH-2024 can cause acute diarrhea and lung pathological injury. The invention also provides a specific fluorescent quantitative PCR primer combination, a vaccine preparation method and a diagnostic kit. The porcine bocavirus cell adapted strain CNH-2024 obtained by separation and identification provided by the invention provides an important technical basis for porcine bocavirus pathogenic mechanism research, vaccine development and clinical diagnosis.

This invention discloses a duck-derived recombinant infectious bronchitis virus and its applications, belonging to the field of viral isolation and application. The virus was deposited at the China Center for Type Culture Collection on December 26, 2024, with accession number CCTCC NO: V202503. The duck-derived recombinant infectious bronchitis virus of this invention is capable of infecting CEF and DEF cells, is convenient for in vitro culture, and is suitable for the production of high-quality inactivated vaccines with high antigen titers. The duck-derived recombinant infectious bronchitis virus DIBVSD2401 of this invention has excellent immunogenicity, and the inactivated vaccine prepared using the duck-derived recombinant infectious bronchitis virus DIBVSD2401 of this invention has good safety and excellent protective effect.