The invention belongs to the technical field of medicines, and particularly relates to a bear gall sugar-free lozenge as well as a preparation method and application thereof. The bear gall sugar-free buccal tablet comprises the following components in parts by weight: 50-100 parts of a bear gall raw material, 250-600 parts of beta-cyclodextrin, 280-680 parts of dextrin, 3-8 parts of steviosin, 4-8 parts of menthol, 4-8 parts of peppermint oil, 2-4 parts of magnesium stearate, 4-8 parts of talcum powder and 3-6 parts of essence. The bear gall raw material is bear bile or bear gall powder. The bear gall is used as a main component, other appropriate auxiliary materials are preferentially matched, the prepared bear gall sugar-free lozenge is good in taste, the original components and style of the bear gall are kept, the natural physiological activity and the specific treatment effect of the bear gall are also kept, the problems of bitter taste, fishy taste and damp taste of the bear gall and the bear gall in a preparation are fundamentally solved, the quality is stable, and the traditional Chinese medicine composition is suitable for children, old people and diabetics to take.