Griseofulvin-producing strain and application thereof
A technology of griseofulvin and Penicillium griseus, applied in the application, fungicides, fungi and other directions, can solve the problems of limited agricultural extension and application, low sugar conversion rate, high fermentation cost, etc., to shorten the fermentation cycle, The process control is simple and the effect of improving the fermentation titer
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Embodiment 1
[0037] Embodiment 1, the screening of griseofulvin producing bacteria F3215
[0038] 1. Collection of soil samples and preliminary screening of Penicillium
[0039] 1. Soil sample collection
[0040] Generally, in fertile soil with more organic matter, the number of microorganisms is the largest. In neutral and alkaline soil, bacteria and actinomycetes are the main species. There are more molds in acidic red soil and forest soil. Orchards, vegetable gardens and wild fruit growing areas, etc. Carbohydrate-rich soils and swamps are rich in yeast and mold.
[0041] Use a sampling shovel to remove floating soil about 5cm from the surface, take 10-15g of soil samples at 5-15cm, put it into a sterilized empty test tube prepared in advance, seal it with a cotton plug, and then seal it with kraft paper. The soil in the north is dry, and samples are taken at 10-20cm. Number the bags and record the location, soil type, time and environmental conditions.
[0042] Generally, the sampl...
Embodiment 2
[0065] Embodiment 2, the breeding of high-yielding griseofulvin bacterial strain FH1816
[0066] On the basis of obtaining the above-mentioned strain F3215 capable of producing griseofulvin, protoplast mutagenesis was used to further increase the yield of griseofulvin, and at the same time, it was an excellent strain with fast growth and stable genetics.
[0067] 1. Preparation of strain F3215 protoplasts
[0068] The glycerol bacteria of the griseofulvin strain F3215 obtained by screening in Example 1 were inoculated onto the slant of Chapeauer's medium, and after culturing at 28°C for 3 days, the slant spores were eluted with sterile physiological saline, and the washed spores were filtered using sterile filter paper. Deliquification obtains spore suspension, and is transferred to culture in Cha's liquid medium by 10% inoculum size (Cas' culture medium slant component comprises: sodium nitrate 0.3g, dipotassium hydrogen phosphate 0.1g, magnesium sulfate (MgSO 4 ·7H 2 O) 0....
Embodiment 3
[0085] Embodiment 3, the fermentation application of griseofulvin bacterial strain FH1816
[0086] According to the biosynthetic characteristics of griseofulvin and the physiological characteristics of the mutant strain FH1816, the fermentation formula and fermentation process were optimized to obtain the optimized formula and control technology suitable for the growth and fermentation production of the mutant strain, and to realize the high yield of the mutant strain. Production of griseofulvin by density scale fermentation.
[0087] 1. Optimization of Griseofulvin Fermentation Medium Formula for Mutant FH1816
[0088] 1. Effects of different carbon sources on the fermentation of the mutant strain FH1816
[0089] In order to compare the effects of different carbon sources on the fermentation level of the mutant strain FH1816, a single factor comparative experiment of seven carbon sources including lactose, glucose, sucrose, maltose syrup, wheat flour, corn flour and rice flo...
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