63 results about "Nosiheptide" patented technology

The invention discloses a brood period feed for a laying hen. The brood period feed for the laying hen comprises the following components in part by weight: 250 to 320 parts of calcium hydrogen phosphate, 250 to 300 parts of mountain flour, 40 to 60 parts of salt, 20 to 40 parts of sodium bicarbonate, 12 to 20 parts of choline, 30 to 40 parts of trace element composite, 8 to 10 parts of multivitamin, 1 to 3 parts of vitamin C, 25 to 30 parts of methionine, 25 to 40 parts of lysine, 50 to 100 parts of fish meal, 40 to 270 parts of zeolite powder, 1 to 3 parts of nosiheptide, 1 to 3 parts of robenidine, 2 to 5 parts of phytase and 2 to 4 parts of multi-enzyme preparation. The feed of the invention is obtained after years of practice; and a nutritive additive and the like are added into the feed, so that the produced brood period feed can obviously enhance the immunity of the laying hen in a brood period, and make the weight and the phalanx length of the laying hen standard at the end of the brood period.

The invention discloses a 817 miscellaneous chicken feed, which comprises the following components in parts by weight: 500-565 parts of corn, 80-150 parts of wheat, 120-270 parts of soya bean dregs, 20-50 parts of 40% cotton dregs, 0-40 parts of peanut dregs, 30-55 parts of DDGS, 15-35 parts of oil, 11-13 parts of rock powder, 6-13 parts of calcium hydrogen phosphate, 2-3 parts of salt, 1.2-1.5 parts of methionine, 4-6.5 parts of lysine sulphate, 0.6-1.0 part of choline chloride, 1.6-2.0 parts of compound trace elements and 0.4 part of compound vitamin. The feed can also comprise the following components in parts by weight: 0.4 part of betaine, 0.2 part of threonine, 0.1 part of enzyme preparation, 0.1 part of ethoxyquinoline, 0.2 part of nosiheptide, 0-0.5 part of dinitolmide and 0.05 part of alpha-aminobutyric acid. The 817 miscellaneous chicken feed provided by the invention is obtained after years of practice and nutritive additives are added into the feed, thus significantly increasing the immunity of the 817 miscellaneous chickens and significantly reducing the feed conversion ratio.

The invention discloses feedstuff for improving immunity of egg-laying chickens. The feedstuff includes the following components in parts by weight: 0.3-1.0 part of ligusticum wallichii, 0.4-1.0 part of Chinese angelica, 0.5-1.0 part of mint, 0.4-1.0 part of radix sophorae flavescentis, 0.3-0.8 part of radix aucklandiae, 0.2-0.8 part of white peony root, 0.5-1.3 parts of codonopsis pilosula, 0.5-1.2 parts of astragalus membranaceus, 0.5-1.0 part of acanthopanax, 0.3-0.8 part of hawthorn, 6-15 parts of vegetable oil, 30-50 parts of fish meal, 40-80 parts of glucose, 120-150 parts of extruded soybeans, 190-250 parts of extruded corn, 231-355 parts of corn flour, 150-200 parts of peeled soybean meal, 14-19 parts of calcium hydrogen phosphate, 11-12 parts of mountain flour, 1.5-1.7 parts of methionine, 1-1.5 parts of lysine, 1-1.2 parts of threonine, 0.1 part of bacillus subtilis, 0.1 part of phytase , 0.1 part of VC, 1 part of choline, 0.1 part of probiotics, 0.1 part of compound enzymes, 2 parts of salt, 2 parts of an acidifier, 2 parts of compound microelements, 2 parts of compound vitamin and 2 parts of nosiheptide. The feedstuff provided by the invention is comprehensive in nutrition, improves the immunity of the egg-laying chickens, and reduces the death and culling rate of the egg-laying chickens.

The invention discloses a preparation method of nosiheptide premix, which comprises the following steps: adding flocculants or filter aids into nosiheptide fermentation broth obtained by fermenting, fully stirring, and carrying out filter pressing or pressing to obtain nosiheptide filter cakes; drying the nosiheptide filter cakes to obtain dry products of nosiheptide mycelium, smashing to obtain fine powder of the nosiheptide mycelium, adding a powdery carrier into the fine powder and evenly mixing to obtain powdery nosiheptide premix; or granulating the powdery nosiheptide premix in a granulator to obtain granular nosiheptide premix; or adding a carrier, an adhesive and water into the dry products of the nosiheptide mycelium to carry out wet granulation, thus obtaining the granular nosiheptide premix. Preparing the nosiheptide premix by adopting the technical scheme of the invention has low requirement on production capability of equipment and low equipment investment, can effectively reduce production cost and has significant economic benefits; and in addition, no organic solvent is used in the preparation method, thus not generating any pollution on environment and having significant social benefits.

The present invention belongs to the field of biomedicine preparing technology, relates to preparation of feed medicine additive, and is especially preparation process of pre-mixed preparation containing Nosiheptide as one kind of sulfur-containing polypeptide. The pre-mixed preparation containing Nosiheptide is prepared through the steps of preparing Nosiheptide generating microbe by means of gene engineering technology, Nosiheptide fermentation, separating extraction and adding supplementary material, stepped dilution, and mixing. The preparation process has raised oxygen utilizing efficiency of streptomycete, raised Nosiheptide yield and lowered production cost.

The invention discloses a laying duck feed which comprises the following components in parts by weight: 100-150 parts of mono-dicalcium phosphate, 200-250 parts of mountain flour, 40-60 parts of salt, 20-40 parts of sodium sulfate, 15-20 parts of choline, 40-45 parts of composite trace elements, 8-10 parts of compound vitamins, 20-30 parts of methionine, 5-8 parts of betaine, 200-250 parts of pine needle meal, 200-300 parts of medical stone, and 8.2 parts of additive, wherein the additive comprises the following components: 1 part of nosiheptide, 2 parts of phytase, 2 parts of compound enzyme preparation, 0.2 part of carophyll red, 1 part of chromium picolinate and 2 parts of probiotics. According to the invention, nutritional additives and the like are added into the feed, so that the feed can meet the needs of laying ducks for high egg laying rate, long egg laying peak time, bright color of egg yolks, and the like, thereby bringing obvious economic benefits.

The invention discloses a method for producing nosiheptide from active streptomyces by virtue of fermentation. The method comprises the following three steps: performing spore culture on a slant culture medium, performing strain culture on a seed tank culture medium, and performing fermentation on a fermentation tank culture medium; and culture or fermentation parameters of each step are optimized. Compared with the prior art, by adopting the method disclosed by the invention, the activation of active streptomyces can be optimized, and the biological activity of the active streptomyces can be improved by virtue of different stages of culture; and the yield of nosiheptide can be increased by more than 60%.

The invention discloses a method for preparing nosiheptide powder, which comprises the following steps: a. carrying out metallic membrane filtration on fermentation liquid containing nosiheptide obtained in fermentation by using a metal pipe type membrane separator and carrying out concentration treatment to remove 20-35 percent of water in the fermentation liquid to obtain concentrated liquid; b. carrying out spray drying treatment on the obtained concentrated liquid to obtain powder containing nosiheptide; adding auxiliary materials into the nosiheptide powder and diluting the nisiheptide powder level by level to prepare nosiheptide preparations with different forms. The invention has the advantages that nosiheptide can be completely extracted from the fermented liquid of nosiheptide, and the method has high yield, low cost and no environmental pollution.

The invention discloses a method for producing nosiheptide employing fermentation of streptomyces actuosus. The method comprises the following three steps: carrying out spore cultivation on a slant culture-medium; carrying out bacterial culture on a seed tank culture medium; and fermenting on a fermentation tank culture medium, wherein culture or fermentation parameters of various steps are optimized. Compared with the prior art, the activation of the streptomyces actuosus is optimized; the biological activity is improved through cultivation of the streptomyces actuosus at different stages; and the yield of the nosiheptide is increased by over 60%.

The invention discloses a universal feed for laying hens. The universal feed comprises the following components in parts by weight: 25-35 parts of mono-dicalcium phosphate, 90-120 parts of rock flour, 15 parts of table salt, 6 parts of sodium bicarbonate, 4 parts of choline, 1.2 parts of compound microelements, 0.5 part of a compound vitamin, 7-10 parts of methionine, 5-15 parts of lysine, 300-350 parts of bean pulp, 200-300 parts of cottonseed meal, 100-150 parts of feather meal, 80-200 parts of peanut meal, 5-25 parts of zeolite powder, 1 part of bacillus subtilis, 1 part of nosiheptide, 1 part of phytase and 1 part of glycine betaine. The universal feed has the advantages that nutritional requirements in brooding, breeding and egg producing periods are simultaneously met; by adequately utilizing unconventional raw materials, the universal feed is low in cost; after the laying hens are fed by the universal feed for 35 days, 70 days and 110 days, the weights of the laying hens respectively reach the standards, the laying rate in high in a laying peak season, and eggs are heavy.

The invention provides a nosiheptide finemeal extracting method. The nosiheptide finemeal extracting method comprises the following steps of adding a filter aid in inactivated nosiheptide fermentation liquor; performing centrifugal treatment to obtain filter residues; adding dichloroethane in the filter residues; stirring for 0.5-1 hour at the temperature of 45-50 DEG C; filtering; keeping the filtrate standing until the filtrate is layered; taking an organic phase to obtain a nosiheptide extracting solution; performing reduced pressure distillation to recycle dichloroethane; adding deionized water at a room temperature; filtering; washing filter cakes with water and drying the washed filter cakes; and grinding the filter cakes to obtain nosiheptide finemeal. The distilled out dichloroethane can be directly recycled and used. The potency of the nosiheptide finemeal obtained by the method is 970 ug / mg, products do not contain mycelium, and yield reaches up to 93%. A nosiheptide extracting technology is simple, extracting efficiency is high, a dichloroethane solvent can be recycled and reused, so that wastewater pollution is reduced, production cost is reduced, and nosiheptide finemeal is suitable to be produced industrially.

The invention relates to a preparation method of pure nosiheptide. The method comprises the steps that nosiheptide flashing powder is used as raw materials, a solvent is added for digestion, the temperature is controlled within 20-60 DEG C, and stirring is performed at the uniform rotating speed of 50-300 r / min for 1-3 h; a leaching solution obtained after the raw materials are processed is separated from solid mushroom dregs, the leaching solution is concentrated on a rotary evaporator at the temperature of 30-70 DEG C, and the organic solvent is recycled to obtain a concentrated solution; water and an elution agent are added into the concentrated solution, stirring is performed at the rotating speed of 100-400 r / min for 1-5 h, and then suction filtration is performed to obtain filter cakes; the filter cakes are placed into a vacuum drying oven, drying is performed at the temperature of 20-70 DEG C and the vacuum degree of minus 0.09 MPa for 6-16 h, and smashing is performed to obtain the pure nosiheptide. The method has the advantages that the production technology is simple, operation is convenient, the production efficiency is high, the product purity is high, and economy and environmental protection are achieved; the extracted nosiheptide can be prepared to obtain nosiheptide raw drugs or nosiheptide premixing agents of different specifications.

The invention discloses a nosiheptide-colistin sulfate premixed agent which comprises 0.5-4 portions of nosiheptide, 1-10 portions of colistin, and the carriers of calcium carbonate, zeolite powder, corn flour, etc. for the rest in every 100 weight portions. The preparation method is as following. The raw materials are added in a mixing machine by the sequence of the carriers, the colistin sulfate, the nosiheptide and the carriers, and mixed evenly to make into powdery nosiheptide-colistin sulfate premixed agent. Or the nosiheptide and the carriers can be mixed fully firstly, then the colistin sulfate is dissolved in the water and sprinkled evenly on the mixture of the nosiheptide and the carriers. After being granulated, dried and screened, the mixture is made into the granular-typed nosiheptide-colistin sulfate premixed agent, the granularity of which is 30-60 mesh. The nosiheptide-colistin sulfate premixed agent of the invention is added in feedstuffs. The content range of the nosiheptide in every kilogram of overhead price feedstuff is 0.5-20mg, and the content range of the colistin sulfate in every kilogram of overhead price feedstuff is 2-40mg.

The invention relates to Streptomyces actuosus LB-16, and further relates to a method for producing Streptomyces actuosus LB-16 and a method for preparing nosiheptide from the Streptomyces actuosus LB-16. In the method for producing the Streptomyces actuosus LB-16, ultraviolet and nitrosoguanidine are adopted for achieving combined mutagenesis for Streptomyces actuosus CN10, and the Streptomyces actuosus LB-16 is obtained through screening. In the method for preparing the nosiheptide from the Streptomyces actuosus LB-16, temperature-variable culture is adopted, the ventilation flow is controlled in a staging mode, and an organic solvent method, resin separation, and ethyl alcohol and water mixed precipitation extraction separation are adopted, so that the nosiheptide is obtained, and the refined nosiheptide is obtained by the adoption of an ion exchange chromatography through separation and purification. The Streptomyces actuosus LB-16 has the advantages of being low in production cost, high in production extraction rate and high in product biological value.

The invention relates to a drug composite for promoting livestock and poultry to grow, which is characterized in that the drug composite comprises 22-26 of codonopsis pilosula, 22-2 of malt, 8-12 of astragalus mongholicus, 16-20 of medicated leaven, 16-20 of hawthorn, 4-7 of angelica sinenisis, and 0.1-1 of nosiheptide according to the weight parts (parts). The preparation method comprises the following steps: all the herbal components are weighed according to the weight parts, ground and sieved, wherein one of the components is well mixed with the nosiheptide, then well mixed with any one ofthe rest components, and the like, and finally well-mixed mixture can be obtained and sub-packaged to obtain the composite.

The invention relates to a novel fluoronosiheptide and a preparation method and application thereof, and particularly discloses a method for preparing 3-methyl-2- indolic acid (MIA) and a fluorine-substituted analogue thereof by fermenting on the basis of escherichia coli. The invention further relates to a type of novel fluorine-substituted nosiheptides (5'-fluoronosiheptide and 6'-fluoronosiheptide) and a preparation method and application thereof. Compounds of the type can be obtained by adding fluorine-substituted tryptophan in the fermenting process of a nosiheptide producing bacterium, i.e. streptomycesactuosus. Compared with a nosiheptide, the 5'-fluoronosiheptide has higher anti-bacteria activity, which is one time higher on Gram-negative bacteria bacillus subtilis.

The invention discloses a feed for laying hens in the reserve period. The feed is composed of following components in parts by weight: 150 to 180 parts of MDCP, 170 to 200 parts of stone powder, 30 to 40 parts of salt, 20 to 40 parts of sodium bicarbonate, 8 to 12 parts of choline, 25 parts of compound trace elements, 5 to 6 parts of compound vitamin, 8 to 12 parts of hydroxyl methionine, 5 to 9 parts of methionine, 25 to 35 parts of lysine, 100 to 150 parts of fish meal, 300 to 330 parts of puffed soybean, 20 to 80 parts of zeolite powder, 1 to 2 parts of nosiheptide, 0.5 to 1 part of dinitolmide, 1 to 2 parts of phytase and 2 to 5 parts of betaine. The weight and neck length of the laying hens fed by the feed exceed the breeding standard in the 70th day, the weight of the laying hens arrives at the standard in the 110th day, and sexual maturity and body maturity of the laying hens are synchronous.

The invention discloses a method for purifying nosiheptide, and particularly relates to a process for purifying nosiheptide by passing through a column. The process comprises the following main steps: preparing a nosiheptide column-passing liquid; and adsorbing, concentrating and drying a macroporous resin column, and the like. According to the method, the purity of a nosiheptide crude product can be effectively improved by selecting proper macroporous resin, a column-passing method and a concentrating and drying method, the content of the prepared nosiheptide can be over 95 percent, and the yield of the nosiheptide can be 70-80 percent. The method has low requirement for instruments and equipment, is simple to operate, and is suitable for large-scale production operation.

The invention provides a system and method for preparing nosiheptide premix particles. The system comprises a storage tank, a vacuum rake dryer and an air compressor, wherein a concentrated solution obtained by removing partial moisture from a nosiheptide fermentation liquid and a binder mixed uniformly with the concentrated solution are filled in the storage tank; a carrier is stored in the vacuum rake dryer; the storage tank and the vacuum rake dryer are connected by the air compressor; the uniformly mixed liquid of the nosiheptide and the binder in the storage tank is delivered into the vacuum rake dryer by the air compressor; then the mixed liquid is uniformly sprayed onto the carrier in the vacuum rake dryer by a nozzle on the vacuum rake dryer; and the carrier with the mixed liquid is dried and mixed in the vacuum rake dryer.

The invention provides a feedstuff for increasing weight of weaned young rabbits. The feedstuff comprises the following constituents by weight percent: 20% of corn, 14% of bean pulp, 4% of yeast powder, 38% of grass meal, 20.5% of bran, 2% of calcium hydrophosphate, 0.5% of table salt and 1% of trace additive, wherein the trace additive is composed of trace elements, multivitamins and defatted rice bran. In the feedstuff for increasing weight of weaned young rabbits provided by the invention, arginine and Nosiheptide are added, so that the feedstuff can improve immune performance and health condition of the rabbits. After using the feedstuff, average weight of the young rabbits at the age of 42 days is more than 900g, daily weight gain is 23-27g, the daily weight gain of the weaned young rabbits fed by normal feedstuff is 16-20g, so the feedstuff provided by the invention improves 35-65%, moreover, arginine and Nosiheptide are added in the feedstuff provided by the invention, thus, the feedstuff can improve organism immune performance and enhance the growth performance. The diet formula for increasing weight of weaned young rabbits provided by the invention has the characteristics of enhancing the immune performance of the young rabbits, improving the health condition of the young rabbits, increasing the weight of the young rabbits after weaning, and the like.

The invention discloses feed for laying hens in a brood period. The feed consists of the following components in parts by weight: 50-80 parts of MDCP, 80-100 parts of stone powder, 20-30 parts of salt, 10-20 parts of baking soda, 8-10 parts of choline, 20 parts of compound trace elements, 4-5 parts of compound vitamin, 0.5-1 part of vitamin C, 15-20 parts of methionine, 20-30 parts of lysine, 100-200 parts of fish meal, 100-200 parts of cottonseed meal, 150-250 parts of bone meal, 100-200 parts of corn germ meal, 30-110 parts of zeolite powder, 1-2 parts of nosiheptide, 0.5-1 part of chloroguanide, 1-2 parts of phytase and 1-2 parts of complex enzyme preparation. The feed provided by the invention is obtained after years of practice, and the invention provides the feed for laying hens in the brood period. After laying hens take the feed provided by the invention, the immunity of the laying hens is significantly enhanced, and the weight and shank length of 70-day laying hens exceed breeding standards.

The present invention provides a method for detecting the content of Nosiheptide preparation. The improvement of the method is that an organic solvent is added beforehand to protect the stability before diluting the Nosiheptide preparation with an oil-insoluble system or ebb-oil-soluble system. The organic solvent which is used for adding beforehand is the organic solvent series which has a certain liposolubility to Nosiheptide, such as DMF, dimethyl sulfoxide, acetone, chloroform, methanol, ethyl and the like. Preferably the organic solvent is DMF. The invention uses adding organic solvent beforehand to protect the stability of the Nosiheptide in dissolving-out and after-treatment to make that the Nosiheptide can be quantitatively and stably transferred to the oil-insoluble dilution thereby the content can be accurately measured or used for the dilution dispersion process with other purpose. The method of the invention evidently improves the accuracy, precision and repeatability of normal detecting method.

The invention discloses a meat duck feed containing jujube powder. The meat duck feed consists of the following components in parts by weight: 450-700 parts of corn, 50 parts of jujube powder, 80-150 parts of wheat middling, 0-150 parts of soybean meal, 50-70 parts of cottonseed meal, 30-50 parts of peanut meal, 15-25 parts of meat and bone meal, 15-40 parts of distillers dried grains with soluble (DDGS), 0-15 parts of soybean oil, 10-13 parts of stone powder, 5-13 parts of calcium hydrophosphate, 2-3 parts of salt, 1-1.6 parts of methionine, 4-6.5 parts of lysine sulfate, 0.6-1.0 part of choline chloride, 2 parts of composite microelement, 0.4 part of composite vitamin, 0.4 part of glycine betaine, 1 part of threonine, 0.1 part of enzymic preparation, 0.1 part of ethoxyquin, 0.2 part of nosiheptide, 0.1 part of bacillus subtilis and 0.05 part of alpha-aminobutyric acid. The meat duck feed is added with nutritional additives and the like, and the feed cost can be reduced by using unconventional raw materials such as the jujube powder, so that the feed conversion ratio of a meat duck is obviously reduced.

The present invention relates to a culture medium for Streptomyces actuosus fermentation production of nosiheptide, and a culture method. According to the present invention, the seed culture medium for Streptomyces actuosus fermentation production of nosiheptide, and the carbon source, the nitrogen source and optimal compatibility ratio in the fermentation culture medium are determined; and with the culture medium formula and the fermentation control process of the present invention, the nosiheptide fermentation unit can be increased, the fermentation cost can be reduced, the influence of the environment on the raw material source and the auxiliary material source can be minimized, the sufficient supply can be ensured, and the stable and efficient production of the nosiheptide can be achieved.

The invention belongs to the field of biotechnology, and relates to a novel antibiotic resistance methyltransgerase, in particular to gene cloning and protein preparation for a nosiheptide resistance ribosomal RNA methyltransgerase, and use of the nosiheptide resistance ribosomal RNA methyltransgerase as a novel antibiotic screening target. The invention provides a bacterial ribosomal RNA methyltransgerase, which comprises a 1-274 position amino acid sequence shown in a sequence table SEQ ID NO:2; and a nucleotide coding sequence comprises a 1-822 position nucleotide sequence shown in SEQ ID NO:1. By utilizing the characteristic that NHR protein methylates an in-vivo RNA target of a bacterium, the bacterial ribosomal RNA methyltransgerase is used as a medicine target for screening novel antibiotics and medicines for reducing nosiheptide resistance. The invention provides new thinking for research of reducing the nosiheptide resistance.

The invention discloses marine Streptomyces sp. and an application of Streptomyces sp. in preparation of nosiheptide. The marine Streptomyces sp. SCSIO 1682 is preserved in the CCTCC (China Center for Type Culture Collection) in Wuhan University, Wuhan City, China on June 6, 2016 and has the preservation number being CCTCC NO: M2016307. A fermentation culture of the marine Streptomyces sp. SCSIO 1682 can be used for preparing nosiheptide shown in the formula (I). A biological preparation method is provided for production and preparation of nosiheptide, and the marine Streptomyces sp. has broad application prospect.

The invention relates to an oil-in-water type neomycin sulfate nosiheptide nanoemulsion antimicrobial medicine which is a nanoemulsion preparation taking neomycin sulfate and nosiheptide as effective components. The antimicrobial medicine comprises the following components in parts by weight: 1 to 20 parts of neomycin sulfate, 15 to 35 parts of surfactant, 0 to 20 parts of cosurfactant, 1 to 25 parts of nosiheptide, 1 to 25 parts of oil and 20 to 70 parts of distilled water. The antimicrobial medicine has a good pharmacological function and an excellent antimicrobial effect via combination of neomycin sulfate and nosiheptide, and is very simple in preparation method and easy to popularize.

The invention discloses a method for detecting nosiheptide contents in nosiheptide fermentation broth, relates to the technical field of detection, and relates to a method for detecting nosiheptide contents. The method comprises the following steps that: a: preparing the reference substance solution of the nosiheptide; b: taking a nosiheptide fermentation broth sample, determining the contents bytaking ethanol water mixed solvent as leach liquor, and then, carrying out ultrasonic extraction to obtain the sample solution of the nosiheptide; and c: injecting the reference substance solution andthe sample solution in sequence for measurement by HPLC (High Performance Liquid Chromatography), recording a chromatogram and a peak area, calculating a correction factor by the mass concentration and the peak area of the main ingredient nosiheptide A in the reference substance solution, utilizing the obtained correction factor to calculate the contents of the nosiheptide A component in the fermentation broth sample, and meanwhile, obtaining the contents of the nosiheptide B component. The method has the advantages of being simple, quick, accurate, efficient and low in toxin and cost, can beused as a fermentation process monitoring method of a nosiheptide production enterprise, and provides monitoring data for a production process in time.

The invention belongs to the technical field of fermentation, and in particular relates to a method and a fermentation medium for fermentation production of nosiheptide. The fermentation medium is prepared from the following components in parts by weight: 0.5-1 part of KNO3, 0.8-1.5 parts of (NH4)2SO4, 3-5 parts of NaCl, 35-45 parts of soybean meal, 1-2.5 parts of soya bean meal, 45-56 parts of corn starch, 0.01-0.02 part of amylase, and 3.5-5 parts of light calcium carbonate. The fermentation medium provided by the invention has the advantages that the raw materials are low in cost and easilyavailable, and the cost of the raw materials can be reduced by about 23.6%. After the fermentation medium provided by the invention is used for fermentation production of nosiheptide, the content ofnosiheptide in the fermented liquid can be improved to 5630ug / mL, the effect is obviously superior to that of the prior art, and thus the method and the fermentation medium can be widely popularized and applied easily.

The invention relates to an extraction method of nosiheptide. The extraction method comprises the following process steps: nosiheptide fermentation liquid atomizing and drying, composite solvent extraction and centrifugation, macroporous resin absorption, acid water hydrolysis, brine precipitation, washing, depressurization and drying. The invention provides a process for extracting the nosiheptide by utilizing composite solvent extraction and macroporous resin de-coloration and purification. According to the method provided by the invention, the extraction efficiency can be improved by 6.5 percent or more, the product purity reaches 96 percent or above, and the economic benefits are favorably improved. According to the method provided by the invention, the wastewater discharge amount is reduced, and the environmental protection treatment is facilitated. The extraction method provided by the invention has the benefits that the solvent corrosion is low, the toxicity is low, the equipment maintenance is facilitated, and the occupational health is facilitated.