63 results about "Nosiheptide" patented technology

The invention discloses a brood period feed for a laying hen. The brood period feed for the laying hen comprises the following components in part by weight: 250 to 320 parts of calcium hydrogen phosphate, 250 to 300 parts of mountain flour, 40 to 60 parts of salt, 20 to 40 parts of sodium bicarbonate, 12 to 20 parts of choline, 30 to 40 parts of trace element composite, 8 to 10 parts of multivitamin, 1 to 3 parts of vitamin C, 25 to 30 parts of methionine, 25 to 40 parts of lysine, 50 to 100 parts of fish meal, 40 to 270 parts of zeolite powder, 1 to 3 parts of nosiheptide, 1 to 3 parts of robenidine, 2 to 5 parts of phytase and 2 to 4 parts of multi-enzyme preparation. The feed of the invention is obtained after years of practice; and a nutritive additive and the like are added into the feed, so that the produced brood period feed can obviously enhance the immunity of the laying hen in a brood period, and make the weight and the phalanx length of the laying hen standard at the end of the brood period.

The invention discloses feedstuff for improving immunity of egg-laying chickens. The feedstuff includes the following components in parts by weight: 0.3-1.0 part of ligusticum wallichii, 0.4-1.0 part of Chinese angelica, 0.5-1.0 part of mint, 0.4-1.0 part of radix sophorae flavescentis, 0.3-0.8 part of radix aucklandiae, 0.2-0.8 part of white peony root, 0.5-1.3 parts of codonopsis pilosula, 0.5-1.2 parts of astragalus membranaceus, 0.5-1.0 part of acanthopanax, 0.3-0.8 part of hawthorn, 6-15 parts of vegetable oil, 30-50 parts of fish meal, 40-80 parts of glucose, 120-150 parts of extruded soybeans, 190-250 parts of extruded corn, 231-355 parts of corn flour, 150-200 parts of peeled soybean meal, 14-19 parts of calcium hydrogen phosphate, 11-12 parts of mountain flour, 1.5-1.7 parts of methionine, 1-1.5 parts of lysine, 1-1.2 parts of threonine, 0.1 part of bacillus subtilis, 0.1 part of phytase , 0.1 part of VC, 1 part of choline, 0.1 part of probiotics, 0.1 part of compound enzymes, 2 parts of salt, 2 parts of an acidifier, 2 parts of compound microelements, 2 parts of compound vitamin and 2 parts of nosiheptide. The feedstuff provided by the invention is comprehensive in nutrition, improves the immunity of the egg-laying chickens, and reduces the death and culling rate of the egg-laying chickens.

The invention discloses a preparation method of nosiheptide premix, which comprises the following steps: adding flocculants or filter aids into nosiheptide fermentation broth obtained by fermenting, fully stirring, and carrying out filter pressing or pressing to obtain nosiheptide filter cakes; drying the nosiheptide filter cakes to obtain dry products of nosiheptide mycelium, smashing to obtain fine powder of the nosiheptide mycelium, adding a powdery carrier into the fine powder and evenly mixing to obtain powdery nosiheptide premix; or granulating the powdery nosiheptide premix in a granulator to obtain granular nosiheptide premix; or adding a carrier, an adhesive and water into the dry products of the nosiheptide mycelium to carry out wet granulation, thus obtaining the granular nosiheptide premix. Preparing the nosiheptide premix by adopting the technical scheme of the invention has low requirement on production capability of equipment and low equipment investment, can effectively reduce production cost and has significant economic benefits; and in addition, no organic solvent is used in the preparation method, thus not generating any pollution on environment and having significant social benefits.

The present invention belongs to the field of biomedicine preparing technology, relates to preparation of feed medicine additive, and is especially preparation process of pre-mixed preparation containing Nosiheptide as one kind of sulfur-containing polypeptide. The pre-mixed preparation containing Nosiheptide is prepared through the steps of preparing Nosiheptide generating microbe by means of gene engineering technology, Nosiheptide fermentation, separating extraction and adding supplementary material, stepped dilution, and mixing. The preparation process has raised oxygen utilizing efficiency of streptomycete, raised Nosiheptide yield and lowered production cost.

The invention discloses a universal feed for laying hens. The universal feed comprises the following components in parts by weight: 25-35 parts of mono-dicalcium phosphate, 90-120 parts of rock flour, 15 parts of table salt, 6 parts of sodium bicarbonate, 4 parts of choline, 1.2 parts of compound microelements, 0.5 part of a compound vitamin, 7-10 parts of methionine, 5-15 parts of lysine, 300-350 parts of bean pulp, 200-300 parts of cottonseed meal, 100-150 parts of feather meal, 80-200 parts of peanut meal, 5-25 parts of zeolite powder, 1 part of bacillus subtilis, 1 part of nosiheptide, 1 part of phytase and 1 part of glycine betaine. The universal feed has the advantages that nutritional requirements in brooding, breeding and egg producing periods are simultaneously met; by adequately utilizing unconventional raw materials, the universal feed is low in cost; after the laying hens are fed by the universal feed for 35 days, 70 days and 110 days, the weights of the laying hens respectively reach the standards, the laying rate in high in a laying peak season, and eggs are heavy.

The invention provides a nosiheptide finemeal extracting method. The nosiheptide finemeal extracting method comprises the following steps of adding a filter aid in inactivated nosiheptide fermentation liquor; performing centrifugal treatment to obtain filter residues; adding dichloroethane in the filter residues; stirring for 0.5-1 hour at the temperature of 45-50 DEG C; filtering; keeping the filtrate standing until the filtrate is layered; taking an organic phase to obtain a nosiheptide extracting solution; performing reduced pressure distillation to recycle dichloroethane; adding deionized water at a room temperature; filtering; washing filter cakes with water and drying the washed filter cakes; and grinding the filter cakes to obtain nosiheptide finemeal. The distilled out dichloroethane can be directly recycled and used. The potency of the nosiheptide finemeal obtained by the method is 970 ug/mg, products do not contain mycelium, and yield reaches up to 93%. A nosiheptide extracting technology is simple, extracting efficiency is high, a dichloroethane solvent can be recycled and reused, so that wastewater pollution is reduced, production cost is reduced, and nosiheptide finemeal is suitable to be produced industrially.

The invention discloses a nosiheptide-colistin sulfate premixed agent which comprises 0.5-4 portions of nosiheptide, 1-10 portions of colistin, and the carriers of calcium carbonate, zeolite powder, corn flour, etc. for the rest in every 100 weight portions. The preparation method is as following. The raw materials are added in a mixing machine by the sequence of the carriers, the colistin sulfate, the nosiheptide and the carriers, and mixed evenly to make into powdery nosiheptide-colistin sulfate premixed agent. Or the nosiheptide and the carriers can be mixed fully firstly, then the colistin sulfate is dissolved in the water and sprinkled evenly on the mixture of the nosiheptide and the carriers. After being granulated, dried and screened, the mixture is made into the granular-typed nosiheptide-colistin sulfate premixed agent, the granularity of which is 30-60 mesh. The nosiheptide-colistin sulfate premixed agent of the invention is added in feedstuffs. The content range of the nosiheptide in every kilogram of overhead price feedstuff is 0.5-20mg, and the content range of the colistin sulfate in every kilogram of overhead price feedstuff is 2-40mg.

The invention relates to Streptomyces actuosus LB-16, and further relates to a method for producing Streptomyces actuosus LB-16 and a method for preparing nosiheptide from the Streptomyces actuosus LB-16. In the method for producing the Streptomyces actuosus LB-16, ultraviolet and nitrosoguanidine are adopted for achieving combined mutagenesis for Streptomyces actuosus CN10, and the Streptomyces actuosus LB-16 is obtained through screening. In the method for preparing the nosiheptide from the Streptomyces actuosus LB-16, temperature-variable culture is adopted, the ventilation flow is controlled in a staging mode, and an organic solvent method, resin separation, and ethyl alcohol and water mixed precipitation extraction separation are adopted, so that the nosiheptide is obtained, and the refined nosiheptide is obtained by the adoption of an ion exchange chromatography through separation and purification. The Streptomyces actuosus LB-16 has the advantages of being low in production cost, high in production extraction rate and high in product biological value.

The invention relates to a drug composite for promoting livestock and poultry to grow, which is characterized in that the drug composite comprises 22-26 of codonopsis pilosula, 22-2 of malt, 8-12 of astragalus mongholicus, 16-20 of medicated leaven, 16-20 of hawthorn, 4-7 of angelica sinenisis, and 0.1-1 of nosiheptide according to the weight parts (parts). The preparation method comprises the following steps: all the herbal components are weighed according to the weight parts, ground and sieved, wherein one of the components is well mixed with the nosiheptide, then well mixed with any one ofthe rest components, and the like, and finally well-mixed mixture can be obtained and sub-packaged to obtain the composite.

The invention relates to a novel fluoronosiheptide and a preparation method and application thereof, and particularly discloses a method for preparing 3-methyl-2- indolic acid (MIA) and a fluorine-substituted analogue thereof by fermenting on the basis of escherichia coli. The invention further relates to a type of novel fluorine-substituted nosiheptides (5'-fluoronosiheptide and 6'-fluoronosiheptide) and a preparation method and application thereof. Compounds of the type can be obtained by adding fluorine-substituted tryptophan in the fermenting process of a nosiheptide producing bacterium, i.e. streptomycesactuosus. Compared with a nosiheptide, the 5'-fluoronosiheptide has higher anti-bacteria activity, which is one time higher on Gram-negative bacteria bacillus subtilis.

The invention discloses a feed for laying hens in the reserve period. The feed is composed of following components in parts by weight: 150 to 180 parts of MDCP, 170 to 200 parts of stone powder, 30 to 40 parts of salt, 20 to 40 parts of sodium bicarbonate, 8 to 12 parts of choline, 25 parts of compound trace elements, 5 to 6 parts of compound vitamin, 8 to 12 parts of hydroxyl methionine, 5 to 9 parts of methionine, 25 to 35 parts of lysine, 100 to 150 parts of fish meal, 300 to 330 parts of puffed soybean, 20 to 80 parts of zeolite powder, 1 to 2 parts of nosiheptide, 0.5 to 1 part of dinitolmide, 1 to 2 parts of phytase and 2 to 5 parts of betaine. The weight and neck length of the laying hens fed by the feed exceed the breeding standard in the 70th day, the weight of the laying hens arrives at the standard in the 110th day, and sexual maturity and body maturity of the laying hens are synchronous.

The invention discloses a method for purifying nosiheptide, and particularly relates to a process for purifying nosiheptide by passing through a column. The process comprises the following main steps: preparing a nosiheptide column-passing liquid; and adsorbing, concentrating and drying a macroporous resin column, and the like. According to the method, the purity of a nosiheptide crude product can be effectively improved by selecting proper macroporous resin, a column-passing method and a concentrating and drying method, the content of the prepared nosiheptide can be over 95 percent, and the yield of the nosiheptide can be 70-80 percent. The method has low requirement for instruments and equipment, is simple to operate, and is suitable for large-scale production operation.

The present invention provides a method for detecting the content of Nosiheptide preparation. The improvement of the method is that an organic solvent is added beforehand to protect the stability before diluting the Nosiheptide preparation with an oil-insoluble system or ebb-oil-soluble system. The organic solvent which is used for adding beforehand is the organic solvent series which has a certain liposolubility to Nosiheptide, such as DMF, dimethyl sulfoxide, acetone, chloroform, methanol, ethyl and the like. Preferably the organic solvent is DMF. The invention uses adding organic solvent beforehand to protect the stability of the Nosiheptide in dissolving-out and after-treatment to make that the Nosiheptide can be quantitatively and stably transferred to the oil-insoluble dilution thereby the content can be accurately measured or used for the dilution dispersion process with other purpose. The method of the invention evidently improves the accuracy, precision and repeatability of normal detecting method.

The invention discloses a meat duck feed containing jujube powder. The meat duck feed consists of the following components in parts by weight: 450-700 parts of corn, 50 parts of jujube powder, 80-150 parts of wheat middling, 0-150 parts of soybean meal, 50-70 parts of cottonseed meal, 30-50 parts of peanut meal, 15-25 parts of meat and bone meal, 15-40 parts of distillers dried grains with soluble (DDGS), 0-15 parts of soybean oil, 10-13 parts of stone powder, 5-13 parts of calcium hydrophosphate, 2-3 parts of salt, 1-1.6 parts of methionine, 4-6.5 parts of lysine sulfate, 0.6-1.0 part of choline chloride, 2 parts of composite microelement, 0.4 part of composite vitamin, 0.4 part of glycine betaine, 1 part of threonine, 0.1 part of enzymic preparation, 0.1 part of ethoxyquin, 0.2 part of nosiheptide, 0.1 part of bacillus subtilis and 0.05 part of alpha-aminobutyric acid. The meat duck feed is added with nutritional additives and the like, and the feed cost can be reduced by using unconventional raw materials such as the jujube powder, so that the feed conversion ratio of a meat duck is obviously reduced.

The present invention relates to a culture medium for Streptomyces actuosus fermentation production of nosiheptide, and a culture method. According to the present invention, the seed culture medium for Streptomyces actuosus fermentation production of nosiheptide, and the carbon source, the nitrogen source and optimal compatibility ratio in the fermentation culture medium are determined; and with the culture medium formula and the fermentation control process of the present invention, the nosiheptide fermentation unit can be increased, the fermentation cost can be reduced, the influence of the environment on the raw material source and the auxiliary material source can be minimized, the sufficient supply can be ensured, and the stable and efficient production of the nosiheptide can be achieved.

The invention belongs to the field of biotechnology, and relates to a novel antibiotic resistance methyltransgerase, in particular to gene cloning and protein preparation for a nosiheptide resistance ribosomal RNA methyltransgerase, and use of the nosiheptide resistance ribosomal RNA methyltransgerase as a novel antibiotic screening target. The invention provides a bacterial ribosomal RNA methyltransgerase, which comprises a 1-274 position amino acid sequence shown in a sequence table SEQ ID NO:2; and a nucleotide coding sequence comprises a 1-822 position nucleotide sequence shown in SEQ ID NO:1. By utilizing the characteristic that NHR protein methylates an in-vivo RNA target of a bacterium, the bacterial ribosomal RNA methyltransgerase is used as a medicine target for screening novel antibiotics and medicines for reducing nosiheptide resistance. The invention provides new thinking for research of reducing the nosiheptide resistance.

The invention discloses a method for detecting nosiheptide contents in nosiheptide fermentation broth, relates to the technical field of detection, and relates to a method for detecting nosiheptide contents. The method comprises the following steps that: a: preparing the reference substance solution of the nosiheptide; b: taking a nosiheptide fermentation broth sample, determining the contents bytaking ethanol water mixed solvent as leach liquor, and then, carrying out ultrasonic extraction to obtain the sample solution of the nosiheptide; and c: injecting the reference substance solution andthe sample solution in sequence for measurement by HPLC (High Performance Liquid Chromatography), recording a chromatogram and a peak area, calculating a correction factor by the mass concentration and the peak area of the main ingredient nosiheptide A in the reference substance solution, utilizing the obtained correction factor to calculate the contents of the nosiheptide A component in the fermentation broth sample, and meanwhile, obtaining the contents of the nosiheptide B component. The method has the advantages of being simple, quick, accurate, efficient and low in toxin and cost, can beused as a fermentation process monitoring method of a nosiheptide production enterprise, and provides monitoring data for a production process in time.

The invention belongs to the technical field of fermentation, and in particular relates to a method and a fermentation medium for fermentation production of nosiheptide. The fermentation medium is prepared from the following components in parts by weight: 0.5-1 part of KNO3, 0.8-1.5 parts of (NH4)2SO4, 3-5 parts of NaCl, 35-45 parts of soybean meal, 1-2.5 parts of soya bean meal, 45-56 parts of corn starch, 0.01-0.02 part of amylase, and 3.5-5 parts of light calcium carbonate. The fermentation medium provided by the invention has the advantages that the raw materials are low in cost and easilyavailable, and the cost of the raw materials can be reduced by about 23.6%. After the fermentation medium provided by the invention is used for fermentation production of nosiheptide, the content ofnosiheptide in the fermented liquid can be improved to 5630ug/mL, the effect is obviously superior to that of the prior art, and thus the method and the fermentation medium can be widely popularized and applied easily.