Biological method for processing spiramycin bacteria residue

A spiramycin and fungus residue technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve problems such as spiramycin fungal residue pollution, achieve safe and reliable performance, increase fermentation titer, cost reduced effect

Inactive Publication Date: 2010-01-13
武汉烁森生态科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to eliminate the toxic factors in the spiramycin residue, improve the protein content of the spiramycin residue, transform the spiramycin residue into a protein source with high safety and excellent performance, and the processed product can be used as The nitrogen source of microbial culture medium or feed protein source is used, so as to fundamentally solve the pollution problem of spiramycin residue and achieve the purpose of resource utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Get dry spiramycin slag (water content 10%) 54kg, wet brewer's grains (water content 70%) 120kg, bran 10kg, make fermentation material moisture be about 50%, then insert 0.75kg compound microbial starter (yeast Bacillus licheniformis 40%, Bacillus licheniformis 20%, Aspergillus niger 40%) and 0.75kg of compound enzyme preparation (protease 30%, cellulase 30%, hemicellulase 20%, glucanase 20%), mix well . The height of fermentation substrate pile is 1m. The fermentation temperature is controlled at 50-55°C, and when the temperature of the fermentation substrate is higher than 55°C, turn over and ventilate to cool down. The fermentation time is 55 hours, dried and inactivated at 80°C for 15 minutes, crushed and packaged to obtain 104kg of product.

Embodiment 2

[0017] Get 150kg of wet spiramycin slag (water content 70%), 40kg of dry brewer's grains (water content 10%), and 10kg of bran, so that the water content of the fermentation substrate is about 55%, and then insert 1.3kg of compound microbial starter (yeast 35%, Bacillus licheniformis 30%, Aspergillus niger 35%) and the compound enzyme preparation (protease 30%, cellulase 30%, hemicellulase 20%, dextranase 20%) of 0.7kg, well mixed. The height of the fermentation substrate pile is 0.9m. The fermentation temperature is controlled at 50-55°C, and when the temperature of the fermentation substrate is higher than 55°C, turn over and ventilate to cool down. The fermentation time is 60 hours, dried and inactivated at 80°C for 15 minutes, crushed and packaged to obtain 100kg of product.

Embodiment 3

[0019] Get dry spiramycin slag (water content 10%) 65kg, dry brewer's grains (water content 10%) 35kg, bran 10kg, insert 0.5kg compound microbial starter (saccharomycete 40%, bacillus licheniformis 20%, Aspergillus niger 40%) and the composite enzyme preparation (protease 30%, cellulase 30%, hemicellulase 20%, glucanase 20%) of 0.5kg, slowly add 95kg water again, make the fermentation substrate water content It is about 54%, mix well. The height of fermentation substrate pile is 1m. The fermentation temperature is controlled at 50-55°C, and when the temperature of the fermentation substrate is higher than 55°C, turn over and ventilate to cool down. The fermentation time is 58 hours, dried and inactivated at 80°C for 15 minutes, crushed and packaged to obtain 100kg of product.

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Abstract

The invention provides a biological method for processing spiramycin bacteria residue. Through the use of a composite microbe fermenting agent and a composite enzyme preparation, the spiramycin bacteria residue is used as a main raw material and added with other auxiliary materials together to form a fermenting substrate for solid fermentation; a fermenting product is subjected to high-temperature drying and inactivation to obtain a product; and the obtained product can be used as a microbe culture medium nitrogen source or a feed protein source. The method has the characteristics of simple process, easy operation, short processing time and high processing efficiency; and the processed product has safety, reliability and excellent performance, thereby solving the problem of pollution of the spiramycin bacteria residue and realizing resource reutilization.

Description

Technical field [0001] The invention provides a biological method for treating spiramycin residue, and relates to the fields of microbiology, bioengineering and fermentation engineering. Background technique [0002] Spiramycin residue is the waste produced by liquid fermentation of spiramycin-producing bacteria. Its components include bacteria, unused medium, medium degradation products, secondary metabolites of producing bacteria, and added Inorganic salts (such as flocculants, filter aids), etc. The spiramycin residue is rich in nutrients and high in crude protein, and is an organic resource with high availability. However, the complexity of the composition of spiramycin residue limits its treatment and reuse. In addition to nutrients, the bacteria residue also contains antibiotic residues and various secondary metabolites produced by spiramycin-producing bacteria. If the fungus residue is directly used as a feed protein source, these substances may cause the accumulat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/00C12P39/00C12N1/00A23K1/00C12R1/645C12R1/10C12R1/685
Inventor 胡杰浦劲松
Owner 武汉烁森生态科技有限公司
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