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Rhizospheric pseudomonad capable of largely producing phenazine-1-carboxylic acid and phenazine-1-amide

A technology of Pseudomonas rhizosphere and Pseudomonas aeruginosa, applied in the field of microorganisms, can solve problems such as unfavorable production

Active Publication Date: 2014-06-04
周莲
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, under general conditions, the fermentation titer of shenazimycin in the wild-type strain M18 is about 0.05-0.1 g / L (Ge Yihe et al., Pseudomonas gacA insertion mutation is effective for pyocyanin and phen Differential regulation of oxazine-1-carboxylic acid synthesis; Acta Microbiology, Vol. 44, No. 6, 2004), detrimental to production

Method used

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  • Rhizospheric pseudomonad capable of largely producing phenazine-1-carboxylic acid and phenazine-1-amide
  • Rhizospheric pseudomonad capable of largely producing phenazine-1-carboxylic acid and phenazine-1-amide
  • Rhizospheric pseudomonad capable of largely producing phenazine-1-carboxylic acid and phenazine-1-amide

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Embodiment

[0033] The bacterial strain of the present invention is a wild-type environmental microorganism isolated and purified when collecting root soil samples in paddy fields in the suburbs of Chongqing. The strain was identified as Pseudomonas aeruginosa (Pseudomonas aeruginosa) PA1201 by 16s rDNA sequence analysis and MIDI SHERLOCK microbial automatic identification system analysis.

[0034] One, bacterial strain of the present invention is cultivated and suitable growth condition:

[0035] The growth temperature range of this strain is 20-42°C, the optimum temperature is 25-30°C, and it can grow in the following three media.

[0036] 1. The basal medium is an inorganic salt medium. The inorganic salt culture medium is specifically, the preparation method of every 1L culture medium comprises the following steps: take 1 liter of deionized water, 4.5 grams of potassium dihydrogen phosphate, 10.5 grams of dipotassium hydrogen phosphate, 2 grams of mannitol, and 2 grams of ammonium su...

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Abstract

The invention discloses a rhizospheric pseudomonad capable of largely producing phenazine-1-carboxylic acid and phenazine-1-amide. The rhizospheric pseudomonad is Pseudomonas aeruginosa PA1201CCTCC No: M2013441. The strain PA1201 is a wild type environmental microorganism separated and purified when a root system soil sample is collected in a rice field in the suburb of Chongqing. The strain disclosed by the invention can be used for stably and efficiently producing the bactericidal agent phenazine-1-carboxylic acid and the novel bactericidal compound phenazine-1-amide, effectively inhibits the growth of rice diseases: Rhizoctonia solani and Xanthomonasoryzae pv.oryzae and can be used for preparing a microbial source pesticide capable of effectively controlling the fungous diseases and bacterial diseases of plants by utilizing the fermentation secondary metabolite of the strain.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Pseudomonas rhizobacteria capable of high-yielding phenazine-1-carboxylic acid and phenazine-1-amide. Background technique [0002] The world's population has grown rapidly in the past half century, while the global arable land area has been decreasing year by year, so the total global food demand continues to increase. In contemporary agricultural production, the application of pesticides has greatly guaranteed food production. In particular, biopesticides, which have gradually emerged in recent years, can prevent and control crop diseases and insect pests on the basis of not polluting the environment, and have made great contributions to improving food production and quality. Biopesticides refer to pesticide preparations that use living organisms (including animals, plants, microorganisms) or their metabolites to kill or inhibit agricultural pests (inc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P17/12A01N43/60A01P1/00C12R1/385
Inventor 周莲何亚文
Owner 周莲
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