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Method for preparing bactericidal agent using antagonizing bacteria M18 strain

A technology of fungicides and bacterial strains, applied in the field of microbial source pesticides, can solve the problems that the content of active ingredients is easily affected by environmental conditions, affecting the stability of plant disease resistance and control effects, and achieves broad-spectrum environmental compatibility and improved antibacterial effects , The effect of increasing the fermentation titer

Inactive Publication Date: 2006-07-19
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biopesticide growth-promoting antagonistic bacteria M18 is a live fungal agent, and the content of the active ingredients synthesized to resist plant diseases is easily affected by environmental conditions, which will affect the ability to resist plant diseases and the stability of the control effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024]The derivative strain M18G of the growth-promoting antagonistic bacteria M18 was inoculated on a plate of glycerol medium, and activated and grown at 26°C for 24 hours, and then the activated M18G strain was transferred to a triangular flask containing glycerol culture medium, and incubated at 26°C. Shaking culture was carried out in a shaker for 14 hours, and the rotation speed of the shaker was 210 rpm. Then continue to transfer, and carry out 3-stage amplified fermentation culture in glycerol culture medium. Finally, it was transferred to the glucose culture solution for amplified fermentation culture, the temperature and rotational speed were unchanged, and the fermentation time was 74 hours to obtain the M18G strain fermentation solution. Wherein, the weight percent of the components contained in the glycerol medium and the glycerol culture solution is: peptone 1.8%, glycerol 1.3%, magnesium sulfate 0.07%, potassium dihydrogen phosphate 0.03%, pH 7.0; the glucose cu...

Embodiment 2

[0029] Inoculate the M18G strain, a derivative strain of the growth-promoting antagonistic bacteria M18, on a plate of glycerol medium, activate and grow at 28°C for 22 hours, and then transfer the activated M18G strain to a triangular flask containing glycerol culture medium for amplification. Cultivate with shaking in a shaker at ℃ for 12 hours, and the speed of the shaker is 220 rpm. Then continue to transfer, carry out 3 stages of amplified fermentation culture in glycerin nutrient solution, finally transfer to glucose nutrient solution and carry out amplified fermentation culture, fermentation temperature and rotating speed are constant, and fermentation time is extended to 72 hours, obtains M18G bacterial strain fermentation liquid. Wherein, the weight percent of the components contained in the glycerin medium and the glycerol culture solution is: peptone 2.0%, glycerin 1.7%, magnesium sulfate 0.1%, potassium dihydrogen phosphate 0.05%, pH7.2; the glucose culture solution...

Embodiment 3

[0035] Inoculate the M18G strain, a derivative strain of the growth-promoting antagonistic bacteria M18, on a plate of glycerol medium, activate and grow at 30°C for 20 hours, and then transfer the activated M18G strain to a triangular flask containing glycerol culture medium to amplify it at 28°C. Cultivate with shaking in a shaking table at ℃ for 12 hours, and the rotating speed of the shaking table is 220 rpm; then continue to transfer, and carry out 3-stage amplified fermentation culture in glycerol culture solution. Finally, it was transferred to the glucose culture solution for amplified fermentation culture, the temperature and rotation speed were constant, and the fermentation time was 70 hours to obtain the M18G strain fermentation solution. Wherein, the weight percent of the components contained in the glycerin medium and the glycerol culture solution is: peptone 2.2%, glycerol 1.7%, magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.01%, pH 6.8; the compositio...

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Abstract

The invention relates to a method for preparing bactericide by using derivative bacterial of growth promoting antagonist bacterial M18, preparing fermentation liquor with high concentration of under phenazine-1-carboxyl acid and pyoluteorin under optimized culture medium and condition by using derivative bacterial M18G and M18R of growth promoting antagonist bacterial M18, preparing the fermentation liquor M18G and M18R into dry powder, double crossing physically the phenazine-1-carboxyl acid in M18G powder and pyoluteorin in M18R according to the weight ratio of 90%-10% and 10%-90%, and finally getting bactericide of high efficiency for preventing plant disease. Compared with current technology, the bactericide provided in this invention is wet powder taking metabolite of microorgsanism as active element but not live bacterial agent, as a result of which the product is characterized by high stability, not easy to be influenced by environment and better prevention and curing effect for multiple plant disease under low consumption.

Description

technical field [0001] The invention relates to a preparation method of a microorganism-derived fungicide, in particular to a method for preparing a fungicide for preventing and controlling plant diseases by utilizing a metabolite of a growth-promoting antagonistic bacteria M18 derivative strain. It belongs to the technical field of microbial source pesticides. Background technique [0002] The reduction in crop production caused by plant diseases accounts for about 25% to 75% of the total output, causing huge economic losses. Taking rice, the main food crop in my country, as an example, the annual planting area is about 400 million mu, and if the yield per mu is 400 kg, if rice diseases cause a 10% reduction in production, the annual economic loss will reach 30 billion yuan. At present, the control of plant diseases in production mainly depends on spraying chemical fungicides in addition to selecting improved varieties and improving cultivation measures. However, most che...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02C12N1/20A01P3/00
Inventor 许煜泉
Owner SHANGHAI JIAO TONG UNIV
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