Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fermentation medium for fermenting riboflavin and using method of fermentation medium

A fermentation medium and riboflavin technology, applied in the field of microorganisms, can solve the problem of low riboflavin fermentation units, and achieve the effects of increasing fermentation titer, increasing supply, and increasing production

Active Publication Date: 2020-02-11
HEBEI SHENGXUE DACHENG PHARMA
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problem of relying on experience rather than theory in the prior art, which makes riboflavin fermentation units low, the present invention provides a method for fermenting riboflavin Fermentation media and methods of use thereof

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Prepare the basic medium, its composition and content are: glucose 10g / L, molasses 60g / L, sodium oleate 25mL / L, yeast powder 8g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate heptahydrate 0.15g / L, zinc gluconate 0.3g / L, and Tween 80 15mL / L were prepared into 9L, respectively packed into three 5L fermentation tanks, each tank was 3L, sterilized for later use, and 10mg of sterile erythromycin was added according to the proportion / L, chloramphenicol 10mg / L;

[0024] Prepare fed-batch medium, its composition and content are: inositol 10g / L, ammonium sulfate 8g / L, glucose 50g / L, magnesium sulfate heptahydrate 250mg / L were prepared 3L, sterilized for standby, and added in proportion to NADPH 10mg / L, tryptophan 190mg / L, alanine 150mg / L, arginine 150mg / L, biotin 150mg / L.

[0025] In the process of riboflavin fermentation and cultivation, insert the riboflavin-producing Bacillus subtilis seed culture into the basal medium prepared in this embodiment according to ...

Embodiment 2

[0028] Prepare the basic medium, its composition and content are: glucose 16g / L, molasses 54g / L, sodium oleate 23mL / L, yeast powder 8g / L, potassium dihydrogen phosphate 1.8g / L, magnesium sulfate heptahydrate 0.15g / L, zinc gluconate 0.5g / L, Tween 80 13mL / L, a total of 9L were prepared, respectively packed into three 5L fermentation tanks, each tank was 3L, sterilized for later use, and sterile erythromycin was added in proportion 10mg / L, chloramphenicol 10mg / L;

[0029] Prepare fed-batch medium, its composition and content are: inositol 8g / L, ammonium sulfate 7g / L, glucose 45g / L, magnesium sulfate heptahydrate 175mg / L, a total of 3L were prepared, sterilized for later use, and added in proportion Sterile NADPH8mg / L, tryptophan 170mg / L, alanine 120mg / L, arginine 130mg / L, biotin 125mg / L.

[0030] In the process of riboflavin fermentation and cultivation, insert the riboflavin-producing Bacillus subtilis seed culture into the basal medium prepared in this embodiment according to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a fermentation medium for fermenting riboflavin and a using method of the fermentation medium, and relates to the technical field of microorganisms. The fermentation medium comprises a basic medium and a fed-batch medium, wherein the basic medium is prepared from the components with content as follows: 10-20 g / L of glucose, 30-60 g / L of molasses, 15-25 mL / L of sodium oleate, 5-8 g / L of yeast powder, 1.5-2 g / L of monopotassium phosphate, 0.1-0.3 g / L of magnesium sulfate heptahydrate, 0.3-0.5 g / L of zinc gluconate, 10-15 mL / L of Tween, 5-15 mg / L of erythromycin and 5-15 mg / L of chloramphenicol; and the fed-batch medium is prepared from the components with content as follows: 5-10 g / L of inositol, 5-8 g / L of ammonium sulfate, 30-50 g / L of glucose and 500-900 mg / L of compound factors. The technical problem that the riboflavin fermentation unit is relatively low due to the fact that the operation relies on experience more than theories in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a fermentation medium for fermenting riboflavin and a using method thereof. Background technique [0002] Riboflavin (vitamin B 2 , Vitamin B 2 , Riboflavin) molecular formula is C 17 h 20 o 6 N 4 , the molecular weight is 376.36. The chemical name is 7,8-dimethyl-10-[(2S,3S,4R)-2,3,4,5-tetrahydroxypentyl]-3,10-dihydrobenzopteridine-2,4 - Diketone, systematically named 7,8-dimethyl-10-(1'-D-ribityl)isoalloxazine [7,8-dimethyl-10-(1'-D-ribityl)soalloxazine]; nucleus Flavin is one of the essential micronutrients for the body and has a wide range of physiological functions. The World Health Organization (WHO) lists it as one of the six major indicators for evaluating human growth and nutritional status. It is widely used in the and feed additive industry; riboflavin in the specification of "US Pharmacopoeia" can be made into tablets for oral administration...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P25/00C12N1/20C12N1/38C12R1/125
CPCC12P25/00C12N1/20C12N1/38
Inventor 贾晓乐王绘砖袁昉徐珍曹建全
Owner HEBEI SHENGXUE DACHENG PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com