Gene for regulating plant height of Gossypium hirsutum, and application thereof

A kind of upland cotton and genetic technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problem of unsatisfactory marker genes of plant height, etc., and achieve the effect of strong specificity

Inactive Publication Date: 2018-08-14
GANSU AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the existing marker genes for plant height are still unsatisfactory

Method used

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  • Gene for regulating plant height of Gossypium hirsutum, and application thereof
  • Gene for regulating plant height of Gossypium hirsutum, and application thereof
  • Gene for regulating plant height of Gossypium hirsutum, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1.1 Test material

[0053] The natural population constructed in this experiment includes 355 representative upland cotton germplasm resources, of which 185 resources come from the early-maturing research group of the Cotton Research Institute of the Chinese Academy of Agricultural Sciences for many years. Germplasm Bank (Anyang, Henan). Among the 355 germplasm resources, 331 variety lines came from various cotton planting ecological regions in China, and 24 variety lines were imported germplasm resources from foreign countries (mainly the United States). According to the source of different ecological regions, the 355 germplasm resources can be divided into five groups: (1) Yellow River Basin Variety Group (YR, 162 accessions); (2) Yangtze River Basin Variety Group (YZR, 51 accessions); (3) Northwest Inland Variety Group (NW, 98 copies); (4) Liaoning Variety Group or Northern Extra Early Maturing Variety Group (LN, 20 copies) and (5) American Variety Group (of which U...

Embodiment 2

[0071] The GhAP1-D3 screened in Example 1 was subjected to VIGS verification function.

[0072] The vector construction of pCLCrVA-pCLCrVB VIGS system:

[0073] The sequences of the designed specific primers are shown in SEQ ID NO.7 and SEQ ID NO.8, specifically, GhAP1-D3V-F: AGGACAAAGCACTGCAAGAACA;

[0074] GhAP1-D3V-R:TCAAGGTGGTGGCGAATCAT.

[0075] 以该引物扩增GhAP1-D3基因的217bp基因沉默的特异片段,“AGGACAAAGCACTGCAAGAACAGAATAACATACTTGCAAAGAAGGAAAAGGAGAAAACTAATGTGGAGCAGGCACATTGGCAGCTGAACAACAATTGCCAAGATTCATCCTCCATGCTTCTGCCCCTTAACATCAGCTCCAATGGAAGGGAGAAGGAAGATAATGAAACCACCAACAGTGGCGTCTTGCTGCCATGGATGATTCGCCACCACCTTGA”,扩增产物如 Figure 5 shown. From Figure 5 It can be seen that the specificity of the primer is strong, and no other bands are amplified.

[0076] Through the Spe I (ACTAGT) and Asc I (GGCGCGCC) restriction sites, replace the sequence between the two restriction sites on the pCLCrVA vector with the C-terminal sequence of GhAP1-D3-217 to obtain pCLCrVA-GhAP1-D3 -217. Transform pCLCrV...

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Abstract

The invention relates to the field of plant height genes, and concretely relates to a gene for regulating the plant height of Gossypium hirsutum, and an application thereof. The base sequence of the CDS of the gene for regulating the plant height of Gossypium hirsutum is represented by SEQ ID NO.1, and a specific base sequence of the gene is represented by SEQ ID NO.2. A large number of SNPs covering the whole genome of Gossypium spp. are developed based on an SLAF-seq reduced representation sequencing technology by utilizing 355 natural populations of Gossypium hirsutum, genetic genes of theplant height are parsed by adopting a GWAS technology through the 3-year and 2-site phenotypic identification of plant height traits, and the genetic screens are screened to obtain the gene for regulating the plant height of Gossypium hirsutum. A result of verification of the functions of the gene by using a virus-induced gene silencing technology shows that the plant height can be controlled by regulating the expression of the gene, so the gene lays a foundation for developing the molecular breeding of the Gossypium hirsutum.

Description

technical field [0001] The invention relates to the field of plant height genes, in particular to genes regulating the plant height of upland cotton and applications thereof. Background technique [0002] Cotton (Gossypium spp.) is the most important fiber crop in the world and one of the most important economic crops in my country. my country is a major cotton-producing country and a major exporter of cotton textiles. Therefore, cotton occupies a very important position in my country's national economy. Upland cotton (Gossypium hirsutum L.) is the cultivated species with the largest planting area. It has high yield, strong adaptability, and excellent fiber quality. It is planted in more than 70 countries and regions in the world, and its planting area accounts for 95% of the total cotton area. above. Cotton plant height is correlated with yield and quality, and is one of the important agronomic traits. What is important is that the high yield of cotton should be centered...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/11C12N15/82A01H5/00A01H6/60
CPCC07K14/415C12N15/8218C12N15/8261
Inventor 宿俊吉喻树迅马麒魏恒玲王寒涛李吉莲马亮王聪聪
Owner GANSU AGRI UNIV
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