Gamma-interferon sandwich ELISA detection method based on recombinant fusion antigen protein

A technology for fusing antigens and antigenic proteins, applied in the field of medical testing and determination, can solve problems such as long detection cycle, poor sensitivity and specificity, and unsuitability for large-scale census
CN103333251AActive Publication Date: 2013-10-02常州同泰生物药业科技股份有限公司

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
常州同泰生物药业科技股份有限公司
Publication Date
2013-10-02

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Abstract

The present invention relates to a gamma-interferon sandwich ELISA detection method based on recombinant fusion antigen protein, further relates to a recombinant fusion antigen protein preparation method and a gamma-interferon monoclonal antibody preparation method, and belongs to the technical field of medical examination determination. According to the present invention, the recombinant fusion antigen protein is formed by linking secretory antigen protein MPB70, antigen protein CFP10 and antigen protein ESAT6 through peptide bonds, the gamma-interferon monoclonal antibody is prepared through immunization of animals, cell fusion, hybridoma cell screening, cloning and purification, and the recombinant fusion antigen protein and the gamma-interferon monoclonal antibody are adopted to establish a sandwich ELISA detection method; and the recombinant fusion antigen protein can rapidly stimulate a bovine blood sample to secret gamma-interferon, and the gamma-interferon monoclonal antibody can quickly and specifically identify bovine gamma-interferon, such that strong sensitivity and strong specificity are provided, and broad application prospects are provided in the field of buffalo tuberculosis diagnosis.
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Description

technical field

[0001] The invention belongs to the technical field of medical examination and determination, in particular to a γ-interferon sandwich ELISA detection method based on recombinant fusion antigen protein. Background technique

[0002] Bovine tuberculosis (tuberculosis, TB) is a chronic wasting disease caused by Mycobacterium bovis, which is distributed worldwide. The World Organization for Animal Health (OIE) lists bovine tuberculosis as a B-type animal disease, and my country lists it as a B-type animal disease. For a long time, people used to think that bovine tuberculosis rarely occurs in buffaloes. However, from 1998 to 2000, bovine tuberculosis with buffaloes as natural hosts broke out in Kroger National Park in South Africa. The infection rate of buffaloes in the south of the park reached 42%. The infection rate was 21%, giving people a hint of the severity of tuberculosis infection in buffalo. In recent years, the rapid development of milk buffalo indu...

Claims

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