Pretreatment method for measuring lead in blood by graphite furnace atomic absorption process

Abstract

A pretreatment method for measuring lead in blood by graphite furnace atomic absorption process belongs to the field of a detection method of lead content in blood and especially relates to a pretreatment method for measuring content of lead in blood by graphite furnace atomic absorption process. The method comprises preparation of a matrix modifier and preparation of a lead standard solution. The matrix modifier contains ammonium biphosphate, Triton and nitric acid. The method comprises the following steps: Step 1, dissolving a freeze-dried bovine blood lead standard substance as required; Step 2, successively adding nitric acid, Triton and ammonium biphosphate, and stabilizing for 1-2 days; Step 3, successively adding whole blood, nitric acid and hydrogen peroxide into the matrix modifier so as to prepare a measuring sample; and Step 4, treating the measuring sample by water bath for 1-2 hours until the sample turns faint yellow from atropurpureus for measurement. The pretreatment method provides a method for accurately measuring content of lead in blood. The pretreatment method is simple to operate, has good stability and high precision, and eliminates interference of blood sample matrix composition.

Description

technical field [0001] The invention belongs to the field of detection methods for lead content in blood, in particular to a pretreatment method for measuring lead content in blood by graphite furnace atomic absorption method. Background technique [0002] In recent years, blood lead incidents have repeatedly occupied the media pages. From Shanghang in Fujian, Jiyuan in Henan, Neijiang in Sichuan, Ningyang in Shandong to Huaining in Anhui, there are so many places where the blood of so many people has seriously exceeded the standard lead. The most commonly used and most effective method is to detect lead in blood. The level of lead in blood can accurately reflect the degree of lead poisoning in the body in the near future, and it is the main means to determine lead poisoning. [0003] Blood lead is a biological exposure indicator that reflects lead exposure, and the determination of blood lead by graphite furnace atomic absorption spectrometry is one of the standard methods....

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Problems solved by technology

[0003] Blood lead is a biological exposure indicator that reflects lead exposure, and the determination of blood lead by graphite furnace atomic absorption spectrometry is one of the standard methods. The existing methods for the determination of blood lead usually take various measures such as digesting the sample port, extracting and separating, or adding a matrix modifier. "Magazine, Volume 28, Issue 21, November 2012, "Improvement of Pretreatment for the Determination of Lead Content in Whole Blood by Graphite Furnace Atomic Absorption Spectrometry" proposed to use 0.1% palladium nitrate as the matrix modifier

The shortcomings of palladium nitrate as a matrix modifier are: 1. The precision is not good enough, the RSD% is relatively large, and the linearity of the curve is not very good; 2. The sample needle is easy to hang liquid during sample injection, which affects the accuracy of the measurement; 3. The price of palladium nitrate is relatively expensive; 4. The metal background is relatively high

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