Pretreatment method for measuring lead in blood by graphite furnace atomic absorption process

A technology of graphite furnace atomic and absorption method, which is applied in the preparation of test samples, etc., which can solve the problems of complicated steps, poor linearity of curves, and influence on accuracy, etc.

Inactive Publication Date: 2015-04-22
STATE GRID CORP OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003] Blood lead is a biological exposure indicator that reflects lead exposure, and the determination of blood lead by graphite furnace atomic absorption spectrometry is one of the standard methods. The existing methods for the determination of blood lead usually take various measures such as digesting the sample port, extracting and separating, or adding a matrix modifier. "Magazine, Volume 28, Issue 21, November 2012, "Improvement of Pretreatment for the Determination of Lead Content in Whole Blood

Method used

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  • Pretreatment method for measuring lead in blood by graphite furnace atomic absorption process

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Experimental program
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Effect test

Embodiment 1

[0017] Weigh according to the volume, 400 μL of matrix modifier; 40 μL of whole blood; 180 μL of 0.1% nitric acid are added to the matrix improvement; slowly add 180 μL of 30% hydrogen peroxide, put the test sample in a water bath for 1.5 hours, and then measure.

Embodiment 2

[0019] Weigh according to the volume, 300 μL of matrix modifier; 30 μL of whole blood; 190 μL of 0.1% nitric acid are added to the matrix improvement; slowly add 180 μL of 30% hydrogen peroxide, put the measurement sample in a water bath for 2 hours, and then measure.

Embodiment 3

[0021] Weigh according to the volume, 600 μL of matrix modifier; 50 μL of whole blood; 200 μL of 0.1% nitric acid are added to the matrix improvement; slowly add 200 μL of 30% hydrogen peroxide, put the measurement sample in a water bath for 2 hours, and then measure.

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Abstract

A pretreatment method for measuring lead in blood by graphite furnace atomic absorption process belongs to the field of a detection method of lead content in blood and especially relates to a pretreatment method for measuring content of lead in blood by graphite furnace atomic absorption process. The method comprises preparation of a matrix modifier and preparation of a lead standard solution. The matrix modifier contains ammonium biphosphate, Triton and nitric acid. The method comprises the following steps: Step 1, dissolving a freeze-dried bovine blood lead standard substance as required; Step 2, successively adding nitric acid, Triton and ammonium biphosphate, and stabilizing for 1-2 days; Step 3, successively adding whole blood, nitric acid and hydrogen peroxide into the matrix modifier so as to prepare a measuring sample; and Step 4, treating the measuring sample by water bath for 1-2 hours until the sample turns faint yellow from atropurpureus for measurement. The pretreatment method provides a method for accurately measuring content of lead in blood. The pretreatment method is simple to operate, has good stability and high precision, and eliminates interference of blood sample matrix composition.

Description

technical field [0001] The invention belongs to the field of detection methods for lead content in blood, in particular to a pretreatment method for measuring lead content in blood by graphite furnace atomic absorption method. Background technique [0002] In recent years, blood lead incidents have repeatedly occupied the media pages. From Shanghang in Fujian, Jiyuan in Henan, Neijiang in Sichuan, Ningyang in Shandong to Huaining in Anhui, there are so many places where the blood of so many people has seriously exceeded the standard lead. The most commonly used and most effective method is to detect lead in blood. The level of lead in blood can accurately reflect the degree of lead poisoning in the body in the near future, and it is the main means to determine lead poisoning. [0003] Blood lead is a biological exposure indicator that reflects lead exposure, and the determination of blood lead by graphite furnace atomic absorption spectrometry is one of the standard methods....

Claims

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Application Information

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IPC IPC(8): G01N1/28
Inventor 曲艺孙成勋朱宝余王鑫李红彦李润琴张赫
Owner STATE GRID CORP OF CHINA
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