Isotonic hemolysin and preparation method thereof, method for treating biological sample and method for detecting leukocyte membrane antigen

A biological sample, hemolysin technology, applied in the field of immunological cell analysis, can solve the problems of inability to achieve hemolysin effect, differences in white blood cell morphology, differences in granulocyte grouping, etc., to reduce scientific research and medical costs, less cell debris, and easier saved effect
CN110579595AActive Publication Date: 2019-12-17GENERAL HOSPITAL OF NUCLEAR IND
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GENERAL HOSPITAL OF NUCLEAR IND
Publication Date
2019-12-17

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Abstract

An isotonic hemolysin comprises an oxidation dissolving agent, a cosolvent, a cell solubilizer, a cell fixing agent, inorganic salts and water. The oxidation dissolving agent is sodium nitrite, and the content of the oxidation dissolving agent is 2-20g / L. The cosolvent comprises one or more of glycerol, diethylene glycol and propylene glycol, and the content of the cosolvent is 0.3-3mmol / L. The cell solubilizer comprises one or more of isobutanol, methanol and ethanol, and the content of the cell solubilizer is 0.1-1mol / L. The cell fixing agent comprises one or more of paraformaldehyde, formaldehyde, ethanol and acetone, and the content of the cell fixing agent is 10-40g / L. The inorganic salts comprise sodium chloride, magnesium chloride and calcium chloride, wherein the content of the sodium chloride is 1-10g / L, the content of the magnesium chloride is 1-100mmol / L, and the content of the calcium chloride is 1-100mmol / L. A preparation method of the hemolysin, a method for treating a biological sample by the hemolysin and a method for detecting a leukocyte membrane antigen by a flow cytometry are presented. After a sample is treated by the hemolysin, each population of leukocytes ispartitioned obviously, leukocyte membrane protein is not damaged, the original biological activity is retained, and red blood cells can be completely split. The result is accurate, and the performance is stable.
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Description

technical field

[0001] The invention relates to the field of immunological cell analysis, in particular to an isotonic hemolysin and a preparation method thereof, a method for processing biological samples and a method for detecting white blood cell membrane antigens. Background technique

[0002] Blood cell analysis is of great value for scientific research, disease prevention, diagnosis and treatment, from the initial study of cell shape, size, quantity to the most recent antigenic composition on the cell surface. Traditional cellular immunity and non-specific immune function detection techniques cannot perform multi-parameter and high-sensitivity analysis on the size, shape, plasma membrane, and internal structure of target cells from the single-cell level. Flow cytometry can quickly measure, sort, Store and display a series of important biophysical and biochemical characteristic parameters of dispersed cells suspended in liquid, and can sort out specified cell subpopulat...

Claims

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