Hemolysin for blood analysis and preparation method thereof and reagent
A technology of blood analysis and hemolysin, which is applied in the field of blood analysis, can solve the problems of not being able to achieve the function of hemolysin multi-type flow cytometry, not being able to meet normal use in time, differences in mononuclear and granulocyte grouping, etc., and achieve a wide range of applications , low cost of use, and obvious effect of partitioning
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Embodiment 1
[0033] Specifically, a reagent for blood analysis including hemolysin and staining solution for blood analysis.
[0034] In this embodiment, the preparation method of the hemolysin for blood analysis comprises the following steps:
[0035] Step S01, weighing 0.5 g of sodium octadecyl sulfate, 0.02 g of ethanol, 2 g of citric acid, 1 g of sodium chloride, 5.1 g of potassium chloride, and 0.1×10 sodium bicarbonate -3 g, standby;
[0036] Step S02, adding 0.5 g of sodium octadecyl sulfate, 2 g of citric acid, 1 g of sodium chloride, and 5.1 g of potassium chloride obtained in step S01 into 600 g of water for full dissolution to obtain an initial mixture;
[0037] Step S03, adding 0.02 g of ethanol to the primary mixed solution prepared in step S02, and then adding 390 g of water to make the volume to 1 liter, to prepare a premixed solution;
[0038] Step S04, adding sodium bicarbonate 0.1×10 -3 g to the premixed solution prepared in step S03, the pH value of the premixed solut...
Embodiment 2
[0044] A reagent for blood analysis comprising hemolysin and staining fluid for blood analysis.
[0045] In this embodiment, the hemolysin used for blood analysis per liter includes the following components: 0.8 g of sodium octadecyl sulfate, 0.04 g of ethanol, 2.5 g of citric acid, 3 g of sodium chloride, and 5.2 g of potassium chloride g. Sodium bicarbonate 0.3×10 -3 g.
[0046] In this embodiment, the preparation method of the hemolysin for blood analysis comprises the following steps:
[0047] Step S01, weighing 0.8 g of sodium octadecyl sulfate, 0.04 g of ethanol, 2.5 g of citric acid, 3 g of sodium chloride, 5.2 g of potassium chloride, and 0.3×10 sodium bicarbonate -3 g, standby;
[0048] Step S02, adding 0.8 g of sodium octadecyl sulfate, 2.5 g of citric acid, 3 g of sodium chloride and 5.2 g of potassium chloride obtained in step S01 into 600 g of water and fully dissolving to obtain an initial mixture ;
[0049] Step S03, adding 0.04 g of ethanol to the primary ...
Embodiment 3
[0056] A reagent for blood analysis comprising hemolysin and staining fluid for blood analysis.
[0057] In this embodiment, the hemolysin used for blood analysis per liter includes the following components: 1.0 g of sodium octadecyl sulfate, 0.06 g of ethanol, 2.8 g of citric acid, 7 g of sodium chloride, and 5.4 g of potassium chloride g. Sodium bicarbonate 0.6×10 -3 g.
[0058] In this embodiment, the preparation method of the hemolysin for blood analysis comprises the following steps:
[0059] Step S01, weighing 1.0 g of sodium octadecyl sulfate, 0.06 g of ethanol, 2.8 g of citric acid, 7 g of sodium chloride, 5.4 g of potassium chloride, and 0.6×10 sodium bicarbonate -3 g, standby;
[0060] Step S02, adding 1.0 g of sodium octadecyl sulfate, 2.8 g of citric acid, 7 g of sodium chloride and 5.4 g of potassium chloride obtained in step S01 into 600 g of water and fully dissolving them to obtain an initial mixture ;
[0061] Step S03, adding 0.06 g of ethanol to the pri...
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