14 results about "In vitro analysis" patented technology

Methods, apparatus, systems and computer-implemented methods configured for identifying viable samples of cellular structures for analysis in an in-vitro microscopy assay. Automatically identifying a first set of samples useful for analysis from a plurality of samples of an assay plate. Generating a set of 2-dimensional (2D) images for each sample in the first set of samples. The set of 2D images for said each sample comprising multiple 2D image slices taken along a z-axis of said each sample. Identifying from the sets of 2D image slices a set of viable samples. Outputting data representative of said set of viable samples for analysis as the set of images.

Provided herein are methods, compositions, systems, kits and uses for integrated, simultaneous spatial multiomic analyses of tissues and samples including chromatin accessibility epigenomic analysis, RNA expression transcriptomic analysis, cell cluster and cell lineage analysis, transcription factor motif analysis, extrachromosomal DNA analysis and mitochondrial DNA analysis.

This invention discloses an antibody targeting FOXA3 and its applications. The antibody or its antigen-binding fragment comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region comprises amino acid sequences HCDR1, HCDR2, and HCDR3 as shown in SEQ ID NO:1, SEQ ID NO:2, and MDY, respectively; the light chain variable region comprises amino acid sequences LCDR1, LCDR2, and LCDR3 as shown in SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7, respectively. The anti-FOXA3 monoclonal antibody of this invention can be used to detect the expression level of FOXA3 protein in cells in vitro, exhibiting good specificity, high antibody concentration and purity. It can be used to analyze the expression level of FOXA3 protein in proteins or tissues in vitro and has good stability.

The present disclosure relates to novel multifunctionalized silicon nanoparticles, to processes for their preparation and to compositions comprising the novel multifunctionalized silicon nanoparticles. The disclosure also relates to the use of the novel multifunctionalized silicon nanoparticles in electrochemiluminescence based detection methods and in the in vitro detection of an analyte. In particular, the disclosure relates to methods for measuring an analyte by in vitro methods employing the novel multifunctionalized silicon nanoparticles.

The present disclosure relates to novel multifunctionalized silicon nanoparticles, to processes for their preparation and to compositions comprising the novel multifunctionalized silicon nanoparticles. The disclosure also relates to the use of the novel multifunctionalized silicon nanoparticles in electrochemiluminescence based detection methods and in the in vitro detection of an analyte. In particular, the disclosure relates to methods for measuring an analyte by in vitro methods employing the novel multifunctionalized silicon nanoparticles.

It is provided a method for analyzing an isolated airway secretion sample of an equine patient in vitro, the method comprising the following steps: a) providing an isolated airway secretion sample originating from an equine patient, b) determining the concentration of at least one protein chosen from the group consisting of mucin-4, polymeric immunoglobulin receptor, carcinoembryonic antigen-related cell adhesion molecule 1, CD177, and vanin 1 in the isolated airway secretion sample, c) determining that the equine patent suffers from equine asthma if the concentration of the at least one protein exceeds a predeterminable threshold.

This invention discloses an antibody targeting ALB and its applications. The antibody or its antigen-binding fragment comprises a heavy chain variable region and a light chain variable region. The heavy chain variable region comprises amino acid sequences HCDR1, HCDR2, and HCDR3 as shown in SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, respectively; the light chain variable region comprises amino acid sequences LCDR1, LCDR2, and LCDR3 as shown in SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8, respectively. The anti-ALB monoclonal antibody of this invention can be used to detect the expression level of ALB protein in cells in vitro, exhibiting good specificity, high antibody concentration and purity. It can also be used to analyze the expression level of ALB protein in proteins or tissues in vitro, demonstrating good stability.

The invention discloses a method for auxiliary assessment of early risk of Alzheimer's disease based on EFEMP1 detection. The method is an in-vitro non-diagnostic analysis method, and comprises the following steps: detecting the biomarker level of EFEMP1 in a biological sample of a subject, and obtaining information for evaluating the AD early risk of the subject based on a detection result. The biomarker comprises an EFEMP1 genotype, an EFEMP1 mRNA (messenger Ribonucleic Acid) expression level or an EFEMP1 protein concentration. When the risk assessment is realized by quantitatively detecting the concentration of the EFEMP1 protein in the plasma, an immunodetection kit containing a capture antibody and a detection antibody aiming at different epitopes of the EFEMP1 protein can be specifically adopted. Objective information for risk judgment is obtained by detecting the protein concentration, genotype or mRNA level of EFEMP1 in a biological sample of a subject, early-stage and non-invasive monitoring of an AD upstream pathological mechanism is achieved, and a new strategy is provided for early warning of diseases.

Embodiments of the present disclosure relate to analyte determining methods and devices (e.g., electrochemical analyte monitoring systems) that have a sensing surface that includes two or more sensing elements disposed laterally to each other, where the sensing surface is on a working electrode of in vivo and / or in vitro analyte sensors, e.g., continuous and / or automatic in vivo monitoring using analyte sensors and / or test strips. Also provided are systems and methods of using the, for example electrochemical, analyte sensors in analyte monitoring.

The application relates to an in-vitro analysis diagnosis detection device and a cartridge valve control assembly. When a first driving mechanism drives a first air pipe and a second air pipe to move to a first position, a first joint and a third joint are respectively used for communicating with a first air hole and a second air hole of a reagent cartridge. When an air path control assembly works in a first working state, a second joint is controlled to communicate with a pressure control mechanism, and a fourth joint communicates with an atmospheric environment. When the air path control assembly works in a second working state, the second joint communicates with the atmospheric environment, and the fourth joint communicates with the pressure control mechanism. Through switching actions of the air path control assembly, the first air hole of the reagent cartridge can communicate with the pressure control mechanism through the first air pipe, and the second air hole of the reagent cartridge can communicate with the pressure control mechanism through the second air pipe. Power can be provided according to process requirements of the reagent cartridge to push sample liquid to transfer between cavities. Meanwhile, the overall structure of the cartridge valve control assembly is relatively simplified.

The invention belongs to the technical field of analysis and detection, and discloses a method for analyzing ferrous ions in a sample and application of the method. The invention particularly relates to a method for analyzing spatial distribution and content of ferrous ions in biological tissues. The method is characterized by comprising the following steps: 1) applying a ferrous ion probe reagent to selectively generate ferrous coordination compound ions with ferrous ions in a sample; (2) detecting the generated ferrous coordination compound ions by adopting a mass spectrum imaging technology; and (3) carrying out qualitative, quantitative and positioning analysis on spatial distribution of ferrous ions (Fe < 2 + >) in the sample by utilizing the collected ion characteristic information of the ferrous coordination compound. The invention further provides specific application of the method in in-vivo and in-vitro analysis.

The present application relates to a kind of in-vitro analysis diagnostic detection device and PCR module, PCR module includes support, temperature rising and falling component and window component.Support is used to install reagent cassette.Temperature rising and falling component includes the substrate on support, the semiconductor refrigerator on substrate, and the heat conduction plate on semiconductor refrigerator.Heat conduction plate is used to be closely contacted with one side of the PCR chamber of reagent cassette.Another side of the PCR chamber is closely contacted with the transparent plate of window component.Optical window is provided with at least one transparent plate.Because the PCR chamber of reagent cassette is slightly inflated under certain pressure, after being compressed by optical window, another side of the PCR chamber is in good contact with heat conduction plate, so that the heat conduction loss between material interface is greatly reduced, the heat conduction efficiency of thermal cycle control of PCR chamber is greatly increased, the temperature of PCR chamber can be accurately controlled, the temperature rising and falling effect is obvious, in addition, the overall structure of PCR module is relatively simple.

The present application relates to a kind of in-vitro analysis diagnosis detection device and optical detection module, optical detection module includes: first support, multiple optical detection monomers and moving mechanism. Multiple optical detection monomers are sequentially spaced on the first support, and optical detection monomer is used to carry out fluorescence detection to sample liquid in PCR chamber. Moving mechanism is connected with the first support, and moving mechanism is used to drive the first support to move, so that multiple optical detection monomers sequentially move to the position opposite to PCR chamber. Relative to the conventional optical detection structure, on the one hand, drive multiple optical detection monomers to sequentially move to the position opposite to PCR chamber along vertical direction or horizontal direction and carry out fluorescence detection, the switching speed is faster, more convenient, can improve fluorescence detection efficiency;On the other hand, the overall structure of optical detection module is relatively simple, and multiple optical detection monomers are independent of each other, can facilitate maintenance work, and service life is longer.

A system for evaluating the plant growth-promoting rhizobacterial properties (PGPR) of endophytic bacteria isolated from Acanthus ilicifolius L., and for assessing plant growth parameters, consisting of: a) an isolation unit for isolating and purifying endophytic bacteria from leaves of Acanthus ilicifolius L., the isolation unit comprising: • Surface sterilization device configured to treat leaf explants successively with Tween20 solution, 70% ethanol and 1% mercuric chloride, • Grinding device for homogenizing leaf tissue with phosphate buffer solution, • Incubation chamber configured for the cultivation of bacterial colonies on nutrient agar at 37°C; b) a characterization unit for the morphological, biochemical and molecular characterization of isolated bacteria, wherein the characterization unit comprises the following: • Equipment for Gram staining, • Equipment for biochemical tests for performing the indole test, the methyl red test, the citrate utilization test, the H2S production test, the gelatin hydrolysis test, the starch hydrolysis test and the catalase test, and • Molecular analysis equipment configured for 16S rRNA sequencing, including DNA isolation apparatus, PCR thermocycler and DNA sequencer; c) an optimization unit configured to analyze the culture conditions for maximum bacterial growth at different temperatures and pH values, the optimization unit comprising a colorimeter configured to measure absorbance at 610 nm to generate bacterial growth curves; d) a PGPR property evaluation unit for assessing the plant growth-promoting potential of isolated bacteria, wherein the PGPR property evaluation unit comprises the following: • Testing equipment for indole-3-acetic acid (IAA) production using the Salkowski color test, • Test apparatus for phosphate solubilization using the Pikovskaya medium • Devices for testing siderophore production using chromazurol S-agar (CAS agar) and • Test apparatus for the activity of 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase using Dworkin and Foster minimal salt medium; and e) a plant growth evaluation unit configured for in vitro analysis of the effects of bacterial inoculum on plant growth parameters such as stem length, root length, leaf length and chlorophyll content.