Animal model for verifying acute virus hepatitis and establishment method of animal model

Abstract

The invention belongs to the field of medicines, and discloses an animal model for verifying acute virus hepatitis and an establishment method of the animal model. The establishment method comprises the following steps: randomly dividing 240 clean male rats which are 240-350g respectively into a normal control group and an acute virus hepatitis model group; performing cell culture; performing inoculation and modeling; performing RNA (Ribonucleic Acid) micro-array gene detection; performing expression experiment and analysis; and establishing an animal model for verifying acute virus hepatitis. The establishment method disclosed by the invention has the characteristics of being simple to operate and low in cost, a drug-resistant liver cancer HepG2 cell model is high in stability, high in autophagy activity and beneficial to in-vitro analysis, can be applied to early-stage diagnosis kits for drug resistance of liver cancer cells and can be used for screening medicines for improving autophagy activity of liver cancer, and thus the drug resistance states of the liver cancer cells can be effectively improved. Tests show that the drug-resistant liver cancer HepG2 cell model established by using the establishment method has insulin resistance longer than 96 hours.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to an animal model for verifying acute viral hepatitis and a construction method thereof. Background technique [0002] Insulin resistance (IR) refers to the decline in the efficiency of insulin in promoting glucose uptake and utilization due to various reasons, that is, the sensitivity of target tissues to insulin response decreases, so that the body compensatoryly secretes excessive insulin to produce hyperinsulinemia Hyperemia, in order to maintain the stability of blood sugar. IR is one of the important mechanisms in the pathogenesis of type II diabetes mellitus (T2DM). Improving IR is the focus of basic research and drug development of T2DM. The liver is one of the target tissues of insulin action and the main site of insulin resistance. Decreased insulin sensitivity can lead to increased hepatic gluconeogenesis and glucose output, and postprandial hyperinsulinemia. High insul...

AI Technical Summary

Problems solved by technology

[0005] To sum up, the problems existing in the existing technology are: the current implantation model of cancerous liver metastases has a high incidence rate, the survival time of the model is short, it cannot adapt to long-term experiments, it cannot simulate the whole process of tumor metastasis, and it does not meet the requirements of clinical liver metastases. The characteristics of the occurrence mechanism and the long induction cycle; most models are difficult to induce, take a long time and do not have long-term stability; and the lack of detection technology for the constructed model cannot accurately verify the effect of the constructed model

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