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Kit for verifying endodontium mesenchymal stem cells

A kind of stem cell and kit technology, which can be used in cell culture active agents, animal cells, vertebrate cells, etc., can solve the problems of long time, affect the results of cell identification, and low induction efficiency, achieve accurate identification results, and improve induction and differentiation. Efficiency, the effect of shortening the time to induce differentiation

Active Publication Date: 2016-08-17
天津欣普赛尔生物医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional mesenchymal stem cell identification methods are relatively mature, but the process of inducing differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results

Method used

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  • Kit for verifying endodontium mesenchymal stem cells
  • Kit for verifying endodontium mesenchymal stem cells
  • Kit for verifying endodontium mesenchymal stem cells

Examples

Experimental program
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Effect test

Embodiment 1

[0061] The adipogenic differentiation induction and detection reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium is containing 2mmoL / L glutamine, 30μg / mL IGF-1, 20mmol / L3 - DMEM medium with glycerol phosphate, 5mmoL / L fluocinolone, 0.5mmoL / L isobutylmethylxanthine, 200μmoL / L indomethacin, 100μmoL / L acetyl CoA, and 10% fetal bovine serum by volume fraction;

[0062] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium is containing 2mmoL / L glutamine, 1mmoL / L fluocinolone, 10μg / mL TGF -β, 10mmol / Lβ-sodium glycerophosphate, 25mmol / L vitamin D3, 50mmol / L vitamin C, DMEM medium with a volume fraction of 10% fetal bovine serum;

[0063] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medi...

Embodiment 2

[0095] The adipogenic differentiation induction and detection reagent set: including 100mL adipogenic induction medium and 10mL adipogenic differentiation detection reagent, the adipogenic induction medium contains 0.2mmoL / L glutamine, 5μg / mLIGF-1, 10mmol / L 3-Glycerol phosphate, 1mmoL / L fluocinolone, 0.1mmoL / L isobutylmethylxanthine, 100μmoL / L indomethacin, 50μmoL / L acetyl CoA, DMEM medium with a volume fraction of 2% fetal bovine serum;

[0096] The osteogenic differentiation induction and detection reagent set: including 100mL osteogenic induction medium and 10mL osteogenic differentiation detection reagent, the osteogenic induction medium contains 0.5mmoL / L glutamine, 0.25mmoL / L fluocinolone, 2μg / L DMEM medium with mL TGF-β, 2mmol / L β-sodium glycerophosphate, 15mmol / L vitamin D3, 10mmol / L vitamin C, and 2% fetal bovine serum by volume fraction;

[0097] The chondrogenic differentiation induction and detection reagent set: including 100mL chondrogenic induction medium and 10...

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Abstract

The invention discloses a kit for verifying endodontium mesenchymal stem cells. The kit comprises a flow phenotype detection reagent set, cell fixation liquid, an ordinary culture medium, an adipogenesis induction and detection reagent set, an osteogenesis induction and detection reagent set and a chondrogenesis induction and detection reagent set. Usually, conventional osteogenesis induction and adipogenesis induction need about 24 days, consumed time is long, and induction efficiency is low; by the kit, the time for osteogenesis induction and adipogenesis induction is shortened to 18 days, induction efficiency is improved, and induction time is shortened; conventional flow detection mainly aims at universal surface markers of mesenchymal stem cells, but the endodontium mesenchymal stem cells have positively-expressed surface markers CD146 which are different from the surface markers of other mesenchymal stem cells; CD146 antibodies are added into the kit, and accordingly, accurate verification results can be acquired.

Description

technical field [0001] The invention relates to a kit, especially a kit for identifying dental pulp mesenchymal stem cells. Background technique [0002] At present, more and more studies are using dental pulp-isolated mesenchymal stem cells for clinical use, and the development prospects of dental pulp mesenchymal stem cells are getting better and better. The International Society for Cell Therapy (ISCT) stipulates that the identification of mesenchymal stem cells includes three aspects: morphology, flow phenotype, and ability to induce differentiation. Traditional mesenchymal stem cell identification methods are relatively mature, but the process of induction and differentiation takes a long time, and the induction efficiency is low, which affects the final cell identification results. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a kit for identifying dental pulp mesenchymal stem cells which improves the in...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569C12N5/077
CPCC12N5/0653C12N5/0654C12N5/0655C12N2500/38C12N2500/40C12N2500/42C12N2500/84C12N2501/105C12N2501/15C12N2501/90C12N2501/998C12N2501/999C12N2502/1364G01N33/56966G01N33/6854
Inventor 刘俊江鲁振宇韩洪起黄文敬张冰晶秦臻徐悦
Owner 天津欣普赛尔生物医药科技有限公司
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