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Lattice-type three-dimensional cell culture support and its manufacturing method and use method

A technology of three-dimensional cells and culture scaffolds, which is applied in tissue culture, biochemical equipment and methods, photolithography process exposure devices, etc. It can solve the problems of analyzing cell culture, affecting research and clinical application, pore size and distribution irregularity, etc. To achieve the effect of improving efficiency, low cost and reducing times

Inactive Publication Date: 2012-06-27
武汉介观生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the pore size and distribution of existing porous three-dimensional materials are irregular, and it is difficult to accurately analyze the microenvironment required for cell culture by using it as a cell culture scaffold, which affects related research and clinical applications.

Method used

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  • Lattice-type three-dimensional cell culture support and its manufacturing method and use method
  • Lattice-type three-dimensional cell culture support and its manufacturing method and use method
  • Lattice-type three-dimensional cell culture support and its manufacturing method and use method

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Experimental program
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Embodiment 1

[0051] Such as figure 1 Shown is an embodiment of a lattice-shaped three-dimensional cell culture scaffold of the present invention. It includes a microporous quartz optical template; the microporous quartz optical template is made of a quartz optical template 5 with a film layer, and the micropores are an array of oblique columns; the quartz optical template 5 with a film layer is on the surface of the quartz optical template 1 After depositing the polymer material film layer 2 used as a sacrificial layer, an optical template of a layer of photosensitive adhesive layer 3 is attached; the oblique pillar array microhole 4 is a microhole made by back exposure 7 and oblique rotation exposure 6 . The ratio of depth to diameter of the oblique column sub-array microholes 4 is greater than or equal to 5 and less than or equal to 20, and 10 is selected in this embodiment. The polymer material film layer 2 of the sacrificial layer is made of water-soluble polymer material. The thickn...

Embodiment 2

[0053] Different from the above-mentioned embodiment 1, the ratio of the depth to the straight length of the oblique pillar sub-array microholes 4 is 5. The thickness of the photosensitive adhesive layer 3 is 50 microns. The photosensitive glue material of the photosensitive glue layer 3 is AZ photosensitive glue.

Embodiment 3

[0055] Different from the above-mentioned embodiment 1, the ratio of the depth to the straight length of the oblique column sub-array microholes 4 is 20. The thickness of the photosensitive adhesive layer 3 is 20 microns. The photosensitive adhesive material of the photosensitive adhesive layer 3 is PMMA photosensitive adhesive.

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Abstract

The invention relates to a lattice-type three-dimensional cell culture support and its manufacturing method and use method. The lattice-type three-dimensional cell culture support comprises a quartz optical template with micropores. The quartz optical template with the micropores is made of a quartz optical template with films. The micropores form an inclined column-type micropore array. The manufacturing method comprises the following steps of preparing the quartz optical template with the films, then preparing the inclined column-type micropore array, and removing the film of a polymer material. The use method of the lattice-type three-dimensional cell culture support comprises high temperature disinfection of the lattice-type three-dimensional cell culture support, surface modification, cell implantation and culture, cell fixation, cell permeabilization and enclosing, cell incubation and cell cleaning. The manufacturing method provided by the invention can adjust aperture sizes, distribution and crystal lattice layer number of the lattice-type three-dimensional cell culture support, can effectively improve scientific and complete experimental data for the tissue engineering research, can improve cell culture efficiency and can reduce the frequency of animal experiments. The lattice-type three-dimensional cell culture support is suitable for large-scale production, has a low cost and can satisfy wide requirements of regenerative medicine.

Description

technical field [0001] The invention relates to a lattice-shaped three-dimensional cell culture scaffold, a manufacturing method thereof, and an application method. The lattice-shaped three-dimensional cell scaffold is widely used in cell culture, stem cell proliferation and differentiation, cell tissue engineering, drug screening, regenerative medicine, microbiology, etc. The application and research of fluid control and nanotechnology belong to the field of biotechnology. Background technique [0002] Cells are the basic unit of life. Cells in human and animal tissue structures are in a complex environment, regulated by microenvironment including soluble cytokines, extracellular matrix and intercellular interactions. Therefore, the in vitro culture of animal cells should not only consider the chemical composition of the cell culture medium, but also the physical and chemical properties of the extracellular matrix. In fact, the behaviors of cell adhesion, reproduction, mig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00A61L27/38A61L27/50G03F7/20
Inventor 张帆石剑陈勇
Owner 武汉介观生物科技有限责任公司
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