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Liquid-based thin-layer cell preserving fluid and preparation method thereof

A technology of thin-layer cells and preservation solution, which is applied in the field of cervical cell pathology detection, can solve the problems of blood cell processing, high clinical price, and cell nucleus shrinkage, and achieve the effect of maintaining pH value, not easy to form agglomerates, and maintaining stability

Inactive Publication Date: 2019-07-23
江苏立峰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a result, solutions specially used for the preservation of exfoliated cells have appeared on the market, such as the preservation solution produced by U.S. Xinbashi. This preservation solution is a reagent used in conjunction with its machine, but the clinical price is expensive, and there are defects in blood cell processing and cell nucleus shrinkage.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The composition of the cell preservation solution of this embodiment includes by mass percentage:

[0034] Sodium phosphate buffer 5.5%, edetate disodium 0.4%, sodium chloride 0.5%, absolute ethanol 14.2%, paraformaldehyde 2%, sodium benzoate 2%, glacial acetic acid 1.2%, polyethylene glycol 2.2 %, ultrapure water 72%.

[0035] in:

[0036] Sodium phosphate buffer is prepared by mixing sodium dihydrogen phosphate and sodium hydrogen phosphate dihydrate and diluting with ultrapure water; the pH of sodium phosphate buffer is 6.8-7.4.

[0037] The purity of absolute ethanol is 99.9%.

[0038] Absolute ethanol, sodium chloride, ethylenediaminetetraacetic acid di-sodium and glacial acetic acid are analytical reagents.

[0039] The preparation method of the cell preservation solution described in this embodiment is:

[0040] 1) Sodium phosphate buffer solution, ethylenediaminetetraacetic acid disodium, sodium chloride, absolute ethanol, paraformaldehyde, sodium benzoate a...

Embodiment 2

[0045] The composition of the cell preservation solution of this embodiment includes by mass percentage:

[0046] Sodium phosphate buffer 5%, EDTA disodium 0.3%, calcium chloride 0.2%, absolute ethanol 12%, sodium benzoate 0.2%, alkyltrimethylamine 5%, polyethylene glycol 3%, ultra-pure Water 74.5%.

[0047] in:

[0048] Sodium phosphate buffer is prepared by mixing sodium dihydrogen phosphate and sodium hydrogen phosphate dihydrate and diluting with ultrapure water; the pH of sodium phosphate buffer is 6.8-7.4.

[0049] The purity of absolute ethanol is 99.9%.

[0050] Absolute ethanol, sodium chloride, ethylenediaminetetraacetic acid di-sodium and glacial acetic acid are analytical reagents.

[0051] The preparation method of the cell preservation solution described in this embodiment is:

[0052] 1) Sodium phosphate buffer solution, ethylenediaminetetraacetic acid disodium, sodium chloride, absolute ethanol, paraformaldehyde, sodium benzoate and glacial acetic acid are ...

Embodiment 3

[0057] The composition of the cell preservation solution of this embodiment includes by mass percentage:

[0058] Tris hydrochloride buffer 6.5%, EDTA disodium 0.5%, sodium bicarbonate 0.8%, paraformaldehyde 15%, sodium benzoate 0.2%, glacial acetic acid 2%, polyethylene glycol 1.5% , Ultrapure water 73.5%.

[0059] in:

[0060]Sodium phosphate buffer is prepared by mixing sodium dihydrogen phosphate and sodium hydrogen phosphate dihydrate and diluting with ultrapure water; the pH of sodium phosphate buffer is 6.8-7.4.

[0061] The purity of absolute ethanol is 99.9%.

[0062] Absolute ethanol, sodium chloride, ethylenediaminetetraacetic acid di-sodium and glacial acetic acid are analytical reagents.

[0063] The preparation method of the cell preservation solution described in this embodiment is:

[0064] 1) Sodium phosphate buffer solution, ethylenediaminetetraacetic acid disodium, sodium chloride, absolute ethanol, paraformaldehyde, sodium benzoate and glacial acetic ac...

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Abstract

The invention provides a liquid-based thin-layer cell preserving fluid. The preserving fluid comprises the following components in percentage by mass: 5-6.5% of a pH buffer solution, 0.3-0.5% of a chelating agent, 0.2-0.8% of an osmotic pressure maintaining agent, 10-26% of a cell fixing agent, 0.1-5% of a preservative, 0.5-10% of a hemolytic agent, 1-4% of a water-soluble adhesive and 66-80% of ultrapure water. The liquid-based thin-layer cell preserving fluid can keep the stability of a cell structure well and keep the pH value stable, so that cells are not easy to agglomerate, and pathological examination is facilitated.

Description

technical field [0001] The invention relates to a liquid-based thin-layer cell preservation solution and a preparation method thereof, belonging to the technical field of cervical cell pathology detection. Background technique [0002] Liquid-based cell preservation solution is the main consumable for liquid-based cytology detection technology. TCT technology has become one of the best recommended methods for screening cervical cancer, providing a very clear diagnostic basis for early diagnosis and treatment of cervical cancer. As a screening method for cervical lesions, liquid-based thin-layer cytology detection technology is used to detect The correct diagnosis rate of abnormal cells has been increased by 13%, and the detection rate of lesions above the squamous epithelium has been increased by 65%, which has advantages that cannot be compared with traditional smears. The most critical reagent in TCT technology is the cell preservation solution. At present, 40%-50% alcoh...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 王勇戴俊峰刘海风戴进娟
Owner 江苏立峰生物科技有限公司
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