Liquid-based thin-layer cell preserving fluid and preparation method thereof

Abstract

The invention discloses a liquid-based thin-layer cell preserving fluid which comprises the following components in percentage by mass: 5%-15% of a pH buffer solution; 0.1%-5% of a mucus dissolving agent, 0.3%-5% of an anticoagulant, 5%-15% of an osmotic pressure regulator, 0.1%-5% of a sterilizing agent, 10%-46% of a cell fixing agent, 0.1%-1.5% of a preservative, 0.5%-10% of a non-toxic hemolytic agent, 1%-4% of an adhesive and 20%-60% of ultrapure water. According to the invention, the application cost is reduced, and cells can be better preserved and treated; through adoption of the liquid-based thin-layer cell preserving fluid, the stability of a cell structure can be well kept, the pH value is kept stable, cells are not prone to agglomeration or spoilage, blood inflammation mucus iseffectively degraded, dyeing is easy, film reading of pathologists is facilitated, and the accuracy of pathological examination is guaranteed. Meanwhile, the mixing and channeling performance is high.

Description

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AI Technical Summary

Benefits of technology

This patented technology describes different ways to stabilize certain chemical substances called buffers (pH) within solutions containing water. These buffers help keep them away from harmful reactions like oxygen radicals while still allowing some liquids to pass freely into their own spaces without being affected by these reactive species. They have been found effective at controllably changing the concentration of specific compounds in the solution based on the desired properties needed. By combining this chemically active ingredients they create stronger antimicrobial compositions that effectively treat diseases associated with oxidized metabolites. Additionally, they use special techniques to protect against damage from ionization processes, reduce protein aggregations, improve crystal formation, detect cancerous tissue, and lyse tumor cells. Overall, this technical results enhance the quality control and analysis capabilities of biomedical samples.

Problems solved by technology

This patented technical problem addressed in this patents relates to finding an ideal way to store high purification level samples containing rare types of tumor cells that may indicate lung cancer development during routine medical procedures like CT scans. Current storage devices suffer issues including lack of sufficient accuracy due to their poor quality control standards, potential contaminating liquids, difficulty handling small sample sizes, and difficulties associated with fixing and storing large amounts of these materials.

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