35 results about "Dual probe" patented technology

The invention provides a double-probe zero-value insulation identification method considering an insulator surface wetting state, and relates to the technical field of power transmission line state detection. The method comprises the steps that S1, two electric field probes are arranged on the surface of an umbrella skirt of an insulator at intervals in the axial direction, and the electric field intensities output by the two electric field probes are marked as E1 and E2 respectively; s2, calculating a deviation rate eta based on the electric field intensities output by the two electric field probes; s3, detecting a surface wetting index W of the insulator; s4, correcting the surface wetting index W to obtain a correction coefficient Kwet; s5, modifying the criterion of the zero value state into eta < lt >; and the zero value state is recognized according to the set threshold value Eta0. Kwet, and Eta0 is a fixed threshold value. According to the method, the accuracy of insulator zero value state recognition can be kept in multiple environment scenes such as rain, fog and moisture, and high robustness and high adaptability are improved for intelligent routing inspection and zero value defect recognition of a power transmission line.

The invention discloses a double-probe electromagnetic logging system which comprises a measuring module, the measuring module is connected with a decoding module, and the decoding module is electrically connected with a computer. The measuring module comprises a shell, the shell is of a hollow cylinder structure, a front end electromagnetic probe, a plurality of side end electromagnetic probes, a time sequence control module and a coding output module are sequentially arranged in the shell in the axial direction, and the coding output module is electrically connected with the decoding module. The invention further discloses a double-probe electromagnetic logging method, which comprises the following steps of: acquiring the diameter of the casing, and setting a transmitting signal voltage; putting the measurement module into a well to obtain a side end sampling signal and a front end sampling signal; when the front-end electromagnetic probe or the side-end electromagnetic probe detects a target, the lowering speed of the measurement module is slowed down; and the computer draws an underground image, and after drawing is completed, well logging is completed. The double-probe electromagnetic well logging system can rapidly and accurately detect the depth position of a metal falling object in a well in different underground environments, so that support is provided for subsequent rapid fishing.

The invention provides a control method and device for double-probe measurement of a double-spindle machine tool and electronic equipment. The method comprises the steps that initial coordinate information of a first probe and initial coordinate information of a second probe are obtained; the first probe and the second probe are controlled to move in the preset machining coordinate system, so that the coordinate information of the first probe is first coordinate information, the coordinate information of the second probe is second coordinate information, and the clamp is controlled to move to the first position in the preset machining coordinate system in the third direction; the first probe is controlled to move by a first preset distance in the direction opposite to the second direction with the first coordinate information as the starting point, and meanwhile the second probe is controlled to move by the first preset distance in the direction opposite to the second direction with the second coordinate information as the starting point; in the moving process, whether the first probe and the second probe make contact with the preset workpiece or not is judged, under the condition that it is determined that the first probe and the second probe make contact with the preset workpiece, measurement execution is completed, and the problem that in the prior art, a double-spindle machine tool is difficult to efficiently measure the workpiece is solved.

The application discloses a kind of automatic imaging detection device and method of clutch type disc piece ultrasonic phased array, belong to nondestructive testing technical field, including ultrasonic detector, motion control device, computer and support frame, two sliding grooves are opened in support frame, probe is arranged on support frame, two sets of clamping mechanisms are arranged on support frame, two sets of clamping mechanisms are connected with probe, the disc piece of being examined is arranged on support frame, semicircular recess is opened in support frame, vertical design is adopted in the application, effectively applicable to double-probe one sends one receives detection scheme, while fully considering the characteristics of ultrasonic phased array detection technology, meet the high-sensitivity ultrasonic detection demand of welded integral blisk weld area, the method has achieved good effect in actual detection work, and ultrasonic phased array rapid imaging detection of integral blisk weld area can be realized.

The invention discloses a steel rail welding seam ultrasonic flaw detection method and device. The method comprises a TOFD detection method, a single-probe reflection detection method and a double-probe K-type detection method. A plurality of pairs of rail side flaw detection probes and different combinations of zero-degree rail surface probes and inclined rail surface probes are arranged on two sides of the inner and outer side surfaces of a steel rail from bottom to top by taking a welding seam as a center, and a TOFD detection method, a single-probe reflection detection method and a double-probe K-type detection method are adopted, so that all-directional and multi-angle detection of a steel rail welding seam area is realized. By means of the method, longitudinal defects, transverse defects and defects at different depth positions in the welding seam can be effectively detected, the leakage detection risk is greatly reduced, and the comprehensiveness and reliability of steel rail welding seam quality detection are ensured.

The invention relates to the technical field of laser positioning, and discloses a double-probe four-laser-beam focus positioning device which comprises a main body, a movement mechanism and a pushing mechanism. The outer surface of the movement mechanism and the inner wall of the body are slidably arranged. When the second rotating plate rotates, the second reflecting plate connected with the second rotating plate can be driven to rotate, and when the second reflecting plate rotates, the angle can be adjusted so that laser can be ejected to the inner wall of the other second reflecting plate; the worker repeats the operation to rotate the second reflecting plate II, so that the laser falling on the inner wall of the reflecting plate II is guided by the angle to fall on the convex mirror and is focused with the other laser to form a point; the situation that laser cannot be focused into a point for positioning due to slight dislocation of the laser head and the convex lens which are in butt joint when the main body moves is avoided, the stability of the device during operation is ensured, and the positioning accuracy of the device through double probes and four laser beam focuses is improved.

The utility model is applicable to the technical field of measurement, and provides a double-probe temperature sensor with a rotary limiting structure, which comprises an equipment main body, the equipment main body comprises a protective tube, sleeves and sensor probes, a pair of sleeves are respectively inserted into two end parts of the protective tube, the sensor probes are provided with limiting grooves, the sleeves are provided with limiting blocks, and the limiting blocks are arranged in the limiting grooves. The outer wall of the sensor probe is provided with limiting plates; the fixing assembly is connected to the sleeve and the sensor probe; according to the utility model, a worker inserts the sensor probe into the upper end part of the sleeve and rotates the sensor probe to ensure that the limiting block is arranged in the limiting groove, and at the moment, the limiting plate on the sensor probe is arranged on the upper end surface of the sleeve; by arranging the limiting block and the limiting plate, the situation that wires in the sensor probe are damaged or connection is loosened due to the fact that the sensor probe is excessively rotated in the installation or maintenance process is avoided, and meanwhile it is ensured that the sensor probe is kept at the fixed position in the sleeve.

This invention relates to a dual-probe switching control system for a coordinate measuring machine (CMM), comprising: a host computer; and a signal switching device having a serial port interface, a power interface, and multiple sets of relay output interfaces. During probe switching, the host computer sends a probe switching signal to the serial port interface, causing one of the normally closed and normally open output interfaces to output a signal to switch to the probe using the corresponding output interface, and sends a signal back to the host computer indicating successful probe switching. The system also sets parameters for the successfully switched probe and receives measurement signals from the host computer. During measurement using the switched probe, the host computer monitors the heartbeat communication signal between the control cabinet and the signal switching device in real time. This invention enables reliable switching between dual probes and ensures reliable operation of the switched probes.

The utility model discloses a double-probe anti-seismic vortex shedding flowmeter, and particularly relates to the technical field of vortex shedding flowmeters, the double-probe anti-seismic vortex shedding flowmeter comprises a measuring tube, flanges fixedly installed at the two ends of the measuring tube and a vortex generating body arranged in the measuring tube, a fixed seat is fixedly installed on the outer top surface of the measuring tube, and a supporting cylinder is fixedly installed on the top surface of the fixed seat. The end, away from the fixing base, of the supporting cylinder is fixedly provided with a flow displayer, and the outer surface of the measuring pipe is sleeved with two damping sleeves. According to the double-probe anti-seismic vortex shedding flowmeter, in actual work, the two damping sleeves are arranged on the outer surface of the measuring pipe in a sleeving mode and fixed through the bolts, then the piston rod and the piston are pushed, nitrogen in the adjusting cylinder is conveyed into the air bag through the connecting pipe, the air bag is expanded, and the measuring pipe is fixed through the connecting pipe. And the air bag absorbs high-frequency vibration energy through elastic deformation, the vibration transmission efficiency is reduced, a user can conveniently observe the pressure in the air bag through the pressure sensor, and therefore vibration resistance is conveniently conducted on pipeline vibration.

The utility model belongs to the field of hearth temperature measuring devices, and particularly relates to a multi-probe mutual compensation type hearth temperature measuring device which comprises a water cooling wall, the outer side of the water cooling wall is fixedly connected with a sealing box, and the inside of the sealing box is fixedly connected with a probe sleeve. According to the utility model, the sealing box is additionally arranged on the water cooling wall, the probe sleeve is additionally arranged on the sealing box, the low-temperature probe is additionally arranged on the probe sleeve, and the high-temperature probe is additionally arranged on the probe sleeve, so that the initial temperature of combustion can be monitored through the additionally arranged low-temperature probe in the process of monitoring the temperature in a non-contact manner; in the monitoring process, the hearth temperature can be monitored in a double-probe mode formed by the high-temperature probe and the low-temperature probe, meanwhile, monitoring can be conducted in a mutual compensation mode of the high-temperature probe and the low-temperature probe, and therefore monitored data can be more complete, and the combustion condition can be effectively known.

The intelligent shower head with the water consumption monitoring function comprises a shower head body, a microcircuit main board module and a probe module, the microcircuit main board module is arranged on one side of the shower head body, and the probe module is in wired connection with the microcircuit main board module; the probe module comprises a first probe and a second probe, the first probe and the second probe are arranged in an internal pipeline of the shower head body, and a set distance is formed between the first probe and the second probe in the pipeline direction. According to the water flow monitoring device matched with the shower head, water flow monitoring is directly carried out at the position of the shower head, additional construction and installation of a flow meter are not needed, the use and installation cost of a user is greatly reduced, and water consumption monitoring accurate to a single time and rapid data acquisition of the user can be achieved through flow speed and time monitoring of the double probes in the shower head.

The invention discloses an oil water content detection device with double-probe self-cleaning capability, and relates to the field of oil quality detection, the oil water content detection device adopts a double-probe structure and carries a probe self-cleaning device, a main control module carries out water content detection based on two different types of probes respectively, and the water content detection accuracy is improved. And calculating a difference index for the two obtained water content predicted values to judge the pollution state of the probe, discarding the water content predicted values and triggering self-cleaning of the probe when the pollution risk of the probe is relatively large, and performing data fusion on the two groups of water content predicted values to obtain a final result when the state of the probe returns to normal. The method has the complementary advantages of sensitivity of microwave detection to dielectric property change of an oil-water mixture and sensitivity of near-infrared detection to specific functional groups of water molecules, can effectively improve the detection precision and stability under the condition of low water content, and improves the sensitivity and anti-interference capability of detection of the water content in oil; the device is suitable for on-line monitoring of oil field pipelines, storage tanks and refining devices.

The invention discloses an on-line replacement conduction type double-probe vortex shedding flowmeter, and belongs to the technical field of vortex shedding flowmeters, the on-line replacement conduction type double-probe vortex shedding flowmeter comprises a measuring pipe body for installing the vortex shedding flowmeter, two ends of the measuring pipe body are respectively provided with a reducing pipe, the other end of each reducing pipe is provided with an installing end seat, and two adjacent installing end seats are connected through auxiliary branches on two sides. And the mounting end seat is provided with an inner closing valve control piece for controlling the opening and closing of the auxiliary branch. By arranging the bypass auxiliary branch and the joint control opening and closing mechanism, when a flow meter core component is replaced, a flow path can be automatically switched to the auxiliary branch, it is ensured that fluid conveying of a main pipeline is not interrupted, and the continuous operation capacity of a system is greatly improved; according to the scheme, modular design is adopted, only the flow meter core body needs to be disassembled and assembled during replacement, the original reducer pipe and the original straight pipe section are reserved, the replacement cost is remarkably reduced, and the requirement for follow-up flow field recalibration is met.

The invention discloses a heat transfer tube eddy current detection double-probe tube aligning device for a heat exchanger and a tube aligning method. The tube aligning device is provided with two supporting legs and two eddy current probe channels for guiding eddy current probes to enter a heat transfer tube; when in use, the pipe aligning device is only required to be placed near a to-be-detected pipe hole, complete alignment is not required, the two supporting legs can keep elastic extension and go deep into the pipe hole under the action of the springs, the pipe aligning device is accurately fixed on the plane of a pipe plate, automatic fine adjustment is performed at the same time, and alignment of two eddy current probe channels and a heat transfer pipe hole is completed. And the eddy current probe is inserted through the eddy current probe channel.

The invention discloses a double-loop chemical waste gas detection device, relates to the technical field of chemical waste gas detection, and solves the problems of tedious switching, detection distortion and poor sealing performance of an existing device. The device comprises a first mounting pipe connected with a chemical waste gas pipeline, a second mounting pipe connected with a purified gas exhaust pipeline and a detection mechanism, the detection mechanism is synchronously driven through a first pneumatic assembly and a second pneumatic assembly, rapid switching between original waste gas and purified gas is achieved, and residual gas can be thoroughly exhausted during switching; the detection assembly adopts a double-probe alternate telescopic design and is matched with filter element pretreatment, so that pollution is avoided; the shell and the end cover are in sealing connection through a flange, key parts are coated with sealing wax, and a one-way air valve is used for assistance, so that multiple sealing is formed; purified gas can be flexibly selected to flow back or be discharged through the three-way reversing valve. The chemical waste gas detection device is efficient in switching, accurate in detection, good in sealing performance, excellent in environmental protection performance, capable of achieving continuous and stable detection before and after chemical waste gas treatment and suitable for various chemical production scenes.

The application discloses a heat treatment hardened layer thickness detection method, device, equipment, medium and product, relates to the engineering nondestructive testing technical field, and the method comprises the following steps: gradually increasing the probe spacing, and monitoring the waveform characteristics of the reflected signal, taking the distortion of the reflected signal as the basis, determining the corresponding probe spacing interval; the probe spacing interval is refined by dichotomy to obtain the probe spacing interval mean; the first peak position time of the direct signal and the second peak position time of the reflected signal are obtained when the probe spacing is in the probe spacing interval mean; the heat treatment hardened layer thickness is determined based on a specific angle, the first peak position time, the second peak position time, the probe spacing interval mean and the sound velocity; the double-probe arrangement mode of one transmitting and one receiving is combined with the ultrasonic diffraction time difference method, the sound path difference between the surface direct signal and the reflected signal is analyzed to calculate the thickness, the technical limitation of the traditional single probe is got rid of, and the application range of the nondestructive testing is improved.

The utility model discloses a split type double probe thickness gauge, including movable frame and shallow, the movable frame top surface is provided with the pole hole, and the pole hole inside is connected with the support rod of passing through, and support rod top surface fixed mounting has the support groove. Advantageous effects: the utility model discloses movable frame and support groove have been used, when the thickness of floor is detected, can place movable frame in the above of floor, place shallow in the below of floor, and staff can push support rod and drive movable frame to move, save the trouble of hand-held emission probe, improve the convenience of moving, at the same time, when the emission probe is sent down, only need to rotate support rod to the baffle bar alignment pole groove, can make the baffle bar pass through the pole groove, and the emission probe is sent down and abuts with the top surface of floor, and the support rod top is supported by the support groove thickness gauge main part, and staff is convenient to observe and use, save the problem that traditional emission probe needs staff to bend down and place, significantly improve the convenience of using.

A dual probe card reader system accommodates Europay®, Mastercard®, and Visa® (EMV) cards in multiple orientations while retaining existing card reader footprints. The system includes a mechanism with two probes positioned to connect with EMV chips in different orientations when cards are facing up. The dual probe mechanism retains other existing card reader parts, enabling detection of EMV chips through either probe position. The system includes an updated probe printed circuit board and modified probe schematics to support both EMV positions. This technique provides enhanced compatibility with chip-based cards while minimizing replacement of existing card reader infrastructure through reuse of current device footprints.

The utility model belongs to the technical field of vortex shedding flowmeters, and particularly relates to a double-probe vortex shedding flowmeter which comprises a measuring tube, flanges are fixedly connected to the two ends of the measuring tube, first connecting seats are fixedly connected to the top and the bottom of a probe fixing seat, and connecting wires are fixedly connected to the end of a first probe and the end of a second probe. Limiting frames are clamped outside the probe fixing seat, limiting rods are in threaded connection with the outer portions of the two-way threaded rods, second limiting columns are fixedly connected to the opposite faces of the two limiting rods, and limiting grooves are formed in the ends of the limiting rods. By arranging the first probe, the second probe, the limiting frame, the two-way threaded rod, the second limiting column and the clamping groove, it can be avoided that due to corrosion of a connector, the overhaul difficulty of the flowmeter is increased; and by arranging the first limiting column and the inserting groove, the limiting rod can move stably, and then the clamping block is inserted into the inserting groove, so that the flowmeter display can be mounted and dismounted.

The present invention discloses a non-contact dual-probe square resistance measurement device and method, which belongs to the field of square resistance measurement technology. In this solution, a controller, an excitation circuit, an auxiliary circuit, a shielding circuit, and an AC power supply are set in the upper and lower probes. When detecting the square resistance value of the film layer on the upper surface of the sample, the excitation circuit of the upper probe and the auxiliary circuit of the lower probe work simultaneously. When detecting the square resistance value of the film layer on the lower surface of the sample, the auxiliary circuit of the upper probe and the excitation circuit of the lower probe work simultaneously. The on and off of the internal circuits of the upper and lower probes are controlled according to the set switching frequency, and the square resistance values ​​of the film layers on the upper and lower surfaces of the sample are measured in turn. By adopting the solution of the present invention, the square resistance of the same position on the upper and lower surfaces of the sample can be measured simultaneously without additional movement or flipping of the sample and the probe, and there is no need to purchase a new set of square resistance meters, nor is there a need for additional installation space or rotation and translation mechanisms, which not only saves costs, but also ensures detection accuracy and improves detection efficiency.

The invention provides an automatic control device and a test method for a high-voltage cable aluminum conductor loop. The device comprises a test circuit module, a voltage regulator, a strong current control box, a manual control weak current box and a wirelessly connected control module, the test circuit module comprises a high-voltage cable aluminum conductor, a terminal, an aluminum connecting pipe, a core-through transformer, a current transformer, an acquisition box and a temperature and current acquisition wire; loRa wireless communication, temperature and current acquisition modules are integrated in the acquisition box, and the temperatures of conductors and connection points are synchronously monitored through a double-probe structure; according to the testing method, the reference temperature theta R is determined through first thermal circulation, then multiple times of automatic thermal circulation are carried out, the temperature is maintained within the range of theta R + / -2 DEG C by adjusting the current, and the shortest heating time is set based on the conductor sectional area. The invention relates to the technical field of power equipment detection, and is specifically applied to the intelligent test of power distribution switch control equipment and cable accessories in the intelligent power grid industry.

This invention discloses an online replacement method for a conductive dual-probe vortex flowmeter, belonging to the technical field of vortex flowmeters. It includes a measuring tube body for mounting the vortex flowmeter, with reduced-diameter pipes at both ends. An installation end seat is mounted on the other end of each reduced-diameter pipe. Adjacent installation end seats are connected by auxiliary branches on both sides. An internal closing valve control is installed on each installation end seat to control the opening and closing of the auxiliary branches. By setting up bypass auxiliary branches and a joint control opening and closing mechanism, this invention automatically switches the flow path to the auxiliary branch when replacing the core components of the flowmeter, ensuring uninterrupted fluid delivery in the main pipeline and greatly improving the system's continuous operation capability. Furthermore, unlike traditional integrated reduced-diameter designs that require replacing both the front and rear reduced-diameter / expanded-diameter pipes, this solution adopts a modular design, requiring only the disassembly and reassembly of the flowmeter core body during replacement, while retaining the original reduced-diameter pipes and straight pipe sections. This significantly reduces replacement costs and the need for subsequent flow field recalibration.

The utility model discloses a support device shared by eddy current and photoelectric detection double probes. The support device comprises a preset base rod, a connecting frame, an adjusting structure and a supporting structure. The preset base rod serves as a supporting foundation, the connecting frame can slide on the base rod, and the adjusting structure and the supporting structure are connected through the first connecting sleeve and the second connecting sleeve respectively. The adjusting structure is composed of a longitudinal adjusting plate, an arc-shaped angle adjusting plate, an extending sliding plate and the like and is matched with a track plate to achieve multi-dimensional adjustment, and a transverse adjusting plate is connected with a supporting structure main rod through an electric protection hose. Adjusting rods of the supporting structure are connected in a hinged mode, angle locking is achieved through a locking pin, and the tightness degree between the adjusting rods and the supporting structure is controlled through a locking piece device. All parts of the device work cooperatively, and the problems that an existing support device is poor in installation adaptability, inflexible in adjustment and insufficient in stability are solved. Aluminum alloy or carbon steel can be selected as a preset base rod material to adapt to various scenes such as a machine tool and a production line.

Methods are provided for conducting a dual-probe assay on a set of nucleic acid targets, including: combining a set of dual probes with a sample composition potentially comprising a set of nucleic acid targets to form a set of cleavable ternary nucleic acid complexes; releasing from the cleavable ternary nucleic acid complex a set of recognition element fragments; hybridizing each of the set of released recognition element fragments to a coded oligonucleotide probe and using resulting hybridized released recognition elements as primers for copying the coded oligonucleotide probe to produce a sect of target-associated codes, wherein each of the coded oligonucleotide probes comprises a code from a set of codes, each code comprises at least one segment encoding one or more symbols that correspond to a sequence of one or more nucleotides; and performing a detection event to identify a set of detected codes of the target-associated codes.

The invention provides a double-probe angle-adjustable mounting bracket for a multi-beam sonar sounding system, which is characterized in that the double-probe angle-adjustable mounting bracket comprises an adapter flange, a sensor mounting plate, a one-to-two flange assembly, an adjusting assembly and a plug, the lower part of the adapter flange is connected with the sensor mounting plate through a bolt; the lower portion of the sensor mounting plate is connected with a one-to-two flange assembly through bolts, the left side and the right side of the one-to-two flange assembly are connected with adjusting assemblies through bolts respectively, and the adjusting assemblies are connected with plugs. Flexible installation of single / double probes is achieved, the orientation angles of the probes can be adjusted on site, various scanning and measuring requirements are met, and the defects that an original structure is fixed in orientation and inconvenient to adjust are overcome.

The utility model discloses a double probe temperature sensor, including temperature sensor main part, the bottom of temperature sensor main part is provided with two wiring port, the inside plug connection of wiring port has detection probe, the upper surface plug connection of detection probe has reinforcing institution, the upper surface of wiring port and detection probe all is provided with positioning hole. This double probe temperature sensor, through the setting of reinforcing institution, when using, respectively with left half arc and right half arc lap joint in detection probe surface, make left half arc and right half arc inside setting antiskid pad close its surface, utilize the positioning bolt again and fix left half arc and right half arc well, cooperate the support provided by the two reinforcing rods of one side setting, effectively enhanced the overall structural strength of detection probe, prevent its deflection or deformation in the installation or use process, thereby ensure that detection probe is always in stable vertical state, guarantee the accuracy and reliability of measurement.

The utility model discloses a novel eddy current displacement sensor double-probe calibration device, and relates to the technical field of sensors, the device comprises a fixing device, a detection assembly and an adjusting device, the adjusting device and the detection assembly are installed on the fixing device; the detection assembly comprises a first detection assembly and a second detection assembly, the first detection assembly comprises a first support and a first sensor probe, the second detection assembly comprises a second support and a second sensor probe, the first sensor probe is fixedly installed on one side of a detected object, and the movable second sensor probe is installed on the other side of the detected object. According to the utility model, the distance between the second probe of the sensor and an object to be measured is controlled by adjusting the micrometer fine tuning sliding table, so that the distance can accurately reach the zero clearance required by the calibration device, and meanwhile, the high-precision digital display ten-thousand-minute ruler is used for stepping the object to be measured, so that the calibration result is more accurate, and the calibration precision is improved.

The present disclosure provides a method for enrichment of at least one target nucleic acid in a library of nucleic acids. This present disclosure is also directed to a faster and easier method of target capture using primer extension reactions that can improve ease of use, turnaround time, and variant allele specificity by designing target enrichment primers to specifically enrich library fragments based on the relative location of the variant base(s) in the primer, the utilization of polymerases with better priming specificity, designing the variant bases in the capture primer, designing the variant bases in the release primer, and / or designing variant specific primers to the both the plus and minus strands of the target library fragment.

The invention provides a single-vessel ultrasonic blood perfusion imaging method and system based on double probes, and the method comprises the steps: injecting a nano-droplet contrast agent into a tested vein or artery, the nano-droplet contrast agent being in a non-activated liquid phase state in a blood vessel; selecting a focus point in the upstream blood vessel A1 as a mark point X of a single upstream artery blood vessel by a user in a man-machine interaction mode; the first probe carries out time-sharing ultrasonic imaging and ultrasonic focusing on the upstream blood vessel A1 and the mark point X through a self-defined ultrasonic sequence, and the ultrasonic focusing activates the liquid-phase nano-droplet contrast agent at the mark point X into gas-phase microbubbles; and then the second probe obtains an ultrasonic video V of the downstream blood perfusion imaging area A2, namely downstream blood perfusion imaging of the marked blood vessel A1. According to the method, the strategy of'division and treatment 'is adopted, blood vessel marking and perfusion imaging tasks are distributed to the double probes respectively, the spatial range and flexibility of blood vessel marking and perfusion imaging are remarkably improved through cooperation, and perfusion analysis of specific blood vessels is better facilitated.

Provided herein are dual probe assays for detecting mutations in amplified target nucleic acids, e.g., for detecting single point mutations associated with drug resistance.