Cytomechanics characteristic measurement equipment and measurement method

Abstract

The invention discloses cytomechanics characteristic measurement equipment. The measurement equipment comprises a laser force-measuring system, a microscopic observation system, a location control system, a signal receiving / converting and digital control system, a postprocessing software system and a liftable objective table; the laser force-measuring system comprises a dual-probe component, a dual-probe component weighted pressure head, a laser source and a location detector; the microscopic observation system comprises a monocular microscope and a multi-light source component; the location control system comprises a location detector displacement controller, a laser source displacement controller, a laser force-measuring system displacement controller, a sample table XY-axle displacementcontroller, a sample table X-axle displacement controller and a nanometer Z-axle displacement controller; the signal receiving / converting and digital control system comprises a displacement table controller, a location detector signal processing unit and a computer; and the postprocessing software system comprises a sensitivity determination module, a thermal curve analysis module, a force curveanalysis module and a global image processing module.

Description

technical field [0001] The invention relates to the field of cell molecular signal interaction in biophysics, in particular to a measuring device and a measuring method for cell mechanical properties. Background technique [0002] Cell mechanical properties are mainly cell Young's modulus and cell surface adhesion properties. In cell mechanics, the Young's modulus of the cell reflects the stiffness of the cell. The stiffness of cells is related to many physiological and pathological states. Cell surface adhesion properties refer to the mechanical properties of the interaction between cell surface molecular receptors and extracellular ligands. Physiological and pathological states of organisms or changes in the microenvironment of cells may affect the adhesion characteristics of cell surfaces. In the physiological environment, there are usually multiple receptors on the cell surface, some receptors are independent of each other, some receptors interact with each other, and...

AI Technical Summary

Problems solved by technology

However, there are still disadvantages in this technique, namely, the small dynamic range of the measurement and the inability to measure the Young's modulus of cells

When measuring, it is necessary to use a micropipette to absorb cells or beads, and then perform a motion cycle of approach-contact-pull back. The force measurement range determined in this process cannot be too large, which limits the force measurement dynamics of this technology. range size (10 1 -10 3 pN)

In addition, the "probes" used in this technology are adsorbed cells or pellets, and a suitable cell model cannot be established, so it is also impossible to calculate a suitable cell Young's modulus result based on the force-distance curve

[0006] In addition, at present, whether it is commercial atomic force microscope or biofilm force probe technology, when measuring samples, only a specific size of petri dish can be selected, which is due to its compact structure design, which is largely limited the size of experimental samples

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