37 results about "Liposome Vesicle" patented technology

This invention describes a novel, two-step method for administering PFC. The first step is designed to block the RES by administration of empty, small, liposomal vesicles (ESV) that are rapidly and preferentially engulfed by macrophages, thereby inhibiting their phagocytosis of subsequently infused PFC emulsions. The second step is the subsequent injection of PFC. ESV are devoid of materials that interfere with the macrophage's metabolic processes and do not impair their ability to clear the circulation of pathogenic organisms. Inhibition of the removal of PFC from the blood stream by the RES will achieve increased circulating PFC, enhanced binding and transport of oxygen throughout the blood stream and consequential reduction of undesirable consequences such as organomegaly and cytokine toxicity.

Combination vaccines for treating or preventing Neisseria meningitidis infection are described. The vaccines include Neisseria meningitidis serogroup B proteoliposomic vesicles and Neissera meningitidis serogroup C conjugated oligosaccharides.

The present invention provides gold nanocluster-liposome composite nanoparticles, which are a gold nanocluster encapsulated with a liposome vesicle, wherein the surface of the liposome vesicle is modified with distearoyl phosphatidylethanolamine-polyethylene glycol, the surface of the gold particle in the gold nanocluster is modified with a penetrating peptide and is bound to Cas9 protein and guiding ribonucleic acid, and the liposome vesicle comprises dioleoyl phosphoethanolamine, 2,3-dioleyloxypropyltrimethyl ammonium chloride and cholesterol. The invention further provides a preparation method and applications of the gold nanocluster-liposome composite nanoparticles. According to the present invention, by using the gold nanocluster-liposome composite nanoparticles, the CRISPR/Cas9 system can be efficiently delivered into cells without any other transfection reagents so as to improve the cell intake rate and the transfection efficiency, such that the gene treatment effects on a variety of tumors can be achieved, and the tracing effect can be achieved.

The invention provides a preparation method of a liposome vesicle based on a microfluidic device. The preparation method comprises the steps of: preparing a microfluidic chip by a soft lithography method; preparing a precursor solution for preparing the liposome vesicle; and using a constant-pressure injection pump to inject the precursor solution into a ''Y''-shaped chip through different injection ports, setting a total flow rate, adjusting the flow ratio of a buffer solution to a phospholipid molecular alcohol solution, and collecting a product at an outlet, wherein the product is the prepared liposome vesicle. The microfluidic chip selected by the invention is a ''Y''-shaped microfluidic chip with a serpentine mixing channel of a square structure, and the chip is prepared by the soft lithography method. By using the chip, based on the principle of self-assembly on an interface, by adjusting the total flow rate and the flow rate ratio of the aqueous buffer solution to the phospholipid molecule ethanol solution, regulation of the size of the liposome vesicle and control on the dimensional uniformity are achieved, and an efficient low-cost method is provided for preparation of artificial cells with uniform properties as well as drug carriers.

The invention relates to a lipidosome medical composition which uses fluticasone propionate as an active component, and a cyst diameter of lipidosome is smaller than 800 nm. The composition comprises 0.025 percent to 0.2 percent of fluticasone propionate as the active component, 0.5 percent to 6 percent of phospholipid, 0 percent to 1 percent of lipophilic additive, 0.01 percent to 1 percent of antioxidant which is used for preserving the medical composition, a pH buffering agent which is used for retaining the pH value from 5 to 7.5, 3 percent to 15 percent of humectant, 20 percent to 30 percent of oil phrase component, 0.01 percent to 0.1 percent of antimicrobial preservative and the balanced of water. The fluticasone propionate lipidosome cream is used for treating skin diseases of human beings or animals.

The invention belongs to the technical field of nanometer supermolecular materials. A liposome vesicle which is non-covalently bonded, good in biocompatibility and high in stability and can realize the functions of targeted transmission and monitoring tracking is prepared by adding amphiphilic p-sulfonated calix [4] arene in proper proportion on the basis that the liposome vesicle is prepared through the traditional phospholipid molecule and cholesterol. The preparation method disclosed by the invention has the advantages of easiness, convenience, small usage amount of main and object raw materials and wide application prospect in the field of targeted transportation of cancer medicines.

The invention relates to a lipidosome medical composition which uses momestasone furoate as an active component, and a cyst diameter of lipidosome is smaller than 800nm. The composition comprises 0.025 percent to 0.2 percent of momestasone furoate as the active component, 0.5 percent to 6 percent of phospholipid, 0 percent to 1 percent of lipophilic additive, 0.01 percent to 1 percent of antioxidant which is used for preserving the medical composition, a pH buffering agent which is used for retaining a pH value from 5 to 7.5, 3 percent to 15 percent of humectant, 20 percent to 30 percent of oil phrase component, 0.01 percent to 0.1 percent of antimicrobial preservative and the balanced of water. The momestasone furoate lipidosome cream is used for treating skin diseases of human beings or animals.

The invention provides PLNPs. The PLNPs are nanoparticles wrapped by liposome vesicles, and the surfaces of the liposome vesicles are modified with distearoyl phosphatidylethanolamine-PEG; the nanoparticles are prepared from lipid particles, chondroitin sulfate and protamine; the liposome vesicles are prepared from dioleoyl phosphatidylethanolamine, N-[1-(2,3-dioleyloxy) propyl]-N,N,N-trimethylammonium chloride and cholesterol. The invention further provides a preparation method of the PLNPs and pharmaceutical composition containing the PLNPs. The PLNPs as a carrier have higher transfection effect. The pharmaceutical composition has better treatment effect on various tumors.

The invention relates to a lipidosome medical composition which uses prednicarbate as an active component, and a cyst diameter of lipidosome is smaller than 800 nm. The composition comprises 0.025 percent to 0.2 percent of prednicarbate as the active component, 0.5 percent to 6 percent of phospholipid, 0 percent to 1 percent of lipophilic additive, 0.01 percent to 1 percent of antioxidant which is used for preserving the medical composition, a pH buffering agent which is used for retaining the pH value from 5 to 7.5, 3 percent to 15 percent of humectant, 20 percent to 30 percent of oil phrase components, 0.01 percent to 0.1 percent of antimicrobial preservative and the balanced of water. The prednicarbate lipidosome cream is used for treating skin diseases of human beings or animals.

The invention provides a DNA zipper molecule modified lipidosome vesicle and a preparation method and application thereof. The lipidosome vesicle is composed of a DNA zipper molecule modified bimolecular layer membrane and a molecular probe wrapped in the bimolecular layer membrane, wherein DNA zipper molecules have a DNA zipper sequence promoting lipidosome fusion, the lipidosome vesicle is rapidly subjected to membrane fusion with an exosome modified by the DNA zipper molecules under the action of a DNA zipper, so that the molecular probe contained in the vesicle is sent into the exosome, and the in-situ, accurate and rapid detection of a nucleic acid-containing biomarker in the exosome is realized. The preparation method of the liposome vesicle comprises the steps: mixing the bimolecular layer membrane and the molecular probe, extruding to obtain the liposome vesicle, and incubating the liposome vesicle with the DNA zipper molecule modified with a lipophilic group. The DNA zipper molecule modified on the surface of the liposome vesicle is actively fused with the exosome, so that the efficiency is higher, the nucleic acid marker to be detected does not need to be separated from the exosome in advance, and the detection effect is better.

A dietary supplement composition has a dispersion including a plurality of liposomal vesicles. The dietary supplement composition includes an active ingredient including one or more hydrophobic nutrient, such as cannabinoid or cannabinoid compound (cannabinoid), and phospholipid contained in the liposomal vesicles. The cannabinoid, which may be combined with cyclodextrin, is incorporated within the liposomal vesicles. The dietary supplement composition may further include one or more of rice bran extract, mannitol and trehalose. A method of preparing such dietary supplement composition includes generating a cannabinoid phase, generating an phospholipid phase, adding the cannabinoid phase and the phospholipid phase in vessel; performing a homogenizing step by mixing the cannabinoid phase and the phospholipid phase so as to form a cannabinoid-phospholipid composition having a plurality of liposomal vesicles having the cannabinoid incorporated therein, wherein the concentration of cannabinoid in the cannabinoid-phospholipid composition is about 10 to 25 mass %.

The invention relates to a lipidosome medical composition which uses methylprednisolone aceponate as an active component, and a cyst diameter of lipidosome is smaller than 800 nm. The composition comprises 0.025 percent to 0.2 percent of methylprednisolone aceponate as the active component, 0.5 percent to 6 percent of phospholipid, 0 percent to 1 percent of lipophilic additive, 0.01 percent to 1 percent of antioxidant which is used for preserving the medical composition, a pH buffering agent which is used for retaining the pH value from 5 to 7.5, 3 percent to 15 percent of humectant, 20 percent to 30 percent of oil phrase component, 0.01 percent to 0.1 percent of antimicrobial preservative and the balanced of water. The methylprednisolone aceponate lipidosome cream is used for treating skin diseases of human beings or animals.

A method of making dietary supplement compositions includes generating an aqueous phase, generating an oil phase, performing a first homogenizing step by mixing the aqueous and oil phases, adding reduced glutathione to the aqueous-oil composition and performing a second homogenizing step by mixing the aqueous-oil composition and reduced glutathione, adding a first flavor to the aqueous-oil-glutathione composition, performing a third homogenizing step by mixing the aqueous-oil-glutathione composition and the first flavor, adding xanthan gum dispersed with glycerin to the aqueous-oil-glutathione-first-flavor composition and performing a fourth homogenizing step by mixing the aqueous-oil-glutathione-first-flavor composition and xanthan gum; adding a second flavor to the aqueous-oil-glutathione-first-flavor-xanthan composition and performing a fifth homogenizing step by mixing the aqueous-oil-glutathione-first-flavor-xanthan composition and the second flavor so as to form a dietary supplement composition. Such method provides a dietary supplement as a dispersion including active ingredients incorporated in liposomal vesicles having a barrier coating of polyethylene glycol.

Disclosed herein is a particle containing an inner liposomal vesicle (ILV) encapsulating a therapeutic agent; an outer liposomal vesicle (OLV) encapsulating the ILV; a membrane fusion-promoting agent;and a pH-altering agent. Also disclosed are methods of delivering a therapeutic agent to a subject comprising: a) providing a herein disclosed particle b) triggering ILV and OLV fusion; and c) releasing the therapeutic agent outside of the OLV. Also disclosed are methods for treating a disease in a subject in need thereof comprising: administering to a subject a herein disclosed particle. Also disclosed are methods to release insulin to an environment comprising increased glucose levels, the method comprising exposing to the environment a herein disclosed particle.

The invention discloses an icaritin liposome oral preparation, and a preparation method thereof. According to the preparation method, the icaritin liposome oral preparation is prepared via thin film dispersion combined ice bath ultrasonic treatment or high pressure homogenization emulsification treatment. 100ml of the icaritin liposome oral preparation contains, 0.2 to 2.4% of icaritin, 0.4 to 12% of lecithin, 0.1 to 1.2% of a film structure conditioning agent, and 0.2% of a nonionic surfactant. The preparation method is capable of solving problems in the prior art via conventional liposome preparation technology that the dissolvability of icaritin in water phase systems is poor, and degradation is easily caused under influences of the external environment; and stability of icaritin in gastrointestinal digestion process is increased. The obtained icaritin liposome oral preparation is regular spherical vesicles; embedding rate is 70% or higher; particle size ranges from 100 to 300nm; particle size distribution is uniform; the preparation method is simple; liposome quality is increased greatly; and the preparation method is suitable for large-scale industrialized production.