3 results about "Liposome Vesicle" patented technology

The invention provides a liposome for protecting mitochondrial vesicles of mesenchymal stem cells as well as a preparation method and application of the liposome, and belongs to the technical field of liposome preparation. The liposome comprises a lipid membrane and a mitochondrial protective agent located in the lipid membrane, preparation raw materials of the lipid membrane comprise DOPC, DOTAP, cholesterol and DSPE-PEG2000, and preparation raw materials of the mitochondrial protective agent comprise human serum albumin, trehalose, glutathione, tryptophan and the like. The liposome has good biocompatibility and safety, and immunogenicity and toxicity risks are reduced. After the lipidosome is fused into the mitochondrial vesicles, the structural integrity, the membrane fluidity and the functional activity of mitochondria can be effectively maintained, mitochondrial swelling and collapse are reduced, and the mitochondrial membrane potential is maintained; the liposome vesicles can be stably stored for 6-8 hours at 25 DEG C, and the short-distance transportation requirement is met. The preparation raw materials of the liposome are low in cost, the preparation method is simple and easy to implement, and the liposome is suitable for large-scale production.

The invention relates to the technical field of sterilization and antifogging, in particular to a microsphere-based antibacterial and antifogging additive, a preparation method thereof, a multifunctional coating and application. The microsphere-based antibacterial and antifogging additive is a liposome vesicle microsphere, and the liposome vesicle microsphere is prepared from an oil-phase raw material and a water-phase raw material; the oil phase raw material comprises one or more of distearoyl phosphatidylcholine, hydrogenated lecithin and cholesterol; the water-phase raw material is a mixture of polyhexamethylene biguanide, polyethyleneimine and benzalkonium chloride; in the water phase raw material, the content of the polyhexamethylene biguanide is 25wt%-45wt%. By optimizing the formula of the microspheres, the efficiency and effect of killing nucleic acid can be remarkably improved, and the fog-resistant and anti-fog effects are achieved.

This invention discloses a method for determining the encapsulation efficiency of amphotericin B liposomes for injection, comprising the following steps: separating the amphotericin B liposome solution for injection using solid-phase extraction adsorption to obtain an encapsulated drug solution and a free drug solution; and determining the drug mass concentration C in the encapsulated drug solution using HPLC. 包封 Drug mass concentration C in free drug solution 游离 And the total drug concentration C in the amphotericin B liposome solution before separation 总 The encapsulation efficiency and recovery rate of amphotericin B liposomes were calculated. This invention utilizes a macroporous copolymer of divinylbenzene and N-vinylpyrrolidone as the adsorbent in a solid-phase extraction adsorption method. Its macroporous structure provides ample adsorption sites while avoiding physical compression and damage to the liposome vesicles. This method achieves the separation of free amphotericin B liposomes from the encapsulated drug, exhibiting good reproducibility, high accuracy, simple operation, and high recovery rate.