Preparation method of multifunctional liposome vesicle

A liposome vesicle, multi-functional technology, applied in liposome delivery, preparations for in vivo tests, medical preparations of non-active ingredients, etc.

Inactive Publication Date: 2014-06-25
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, amphiphilic sulfonated calixarene stands out among many calixarene derivatives due to its excel

Method used

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  • Preparation method of multifunctional liposome vesicle
  • Preparation method of multifunctional liposome vesicle
  • Preparation method of multifunctional liposome vesicle

Examples

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Embodiment 1

[0026] A preparation method of the multifunctional liposome vesicles, the steps are as follows:

[0027] 1) Dissolve DPPC and cholesterol in chloroform according to the ratio of 3:1, so that the final concentration of DPPC is 5 mmol / L. Dry under vacuum for at least 6 hours;

[0028] 2) Add SC4AB aqueous solution with a concentration of 0.25 mmol / L equal to the volume of chloroform to the above liposome film, stir at 55-60°C for 30 minutes, then sonicate in a water bath for 30 minutes, and finally use a 220 nm filter to remove large lipid particles Plastids can be used to prepare multifunctional liposome vesicles.

Embodiment 2

[0030] A preparation method of the multifunctional liposome vesicles, the steps are as follows:

[0031] 1) Dissolve DPPC and cholesterol in chloroform according to the ratio of 3:1, so that the final concentration of DPPC is 5 mmol / L. Dry under vacuum for at least 6 hours;

[0032] 2) Add SC4AB aqueous solution with a concentration of 0.5 mmol / L equal to the volume of chloroform to the above liposome film, stir at 55-60°C for 30 minutes, then sonicate in a water bath for 30 minutes, and finally use a 220 nm filter to remove large lipid particles Plastids can be used to prepare multifunctional liposome vesicles.

Embodiment 3

[0047] A method for functional derivatization of the multifunctional liposome vesicles, the steps are as follows:

[0048] The multifunctional liposome vesicle solution prepared in Example 2 was diluted 10 times, and methyl viologen was added to the final concentration of 0.05 mmol / L, and mixed evenly.

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Abstract

The invention belongs to the technical field of nanometer supermolecular materials. A liposome vesicle which is non-covalently bonded, good in biocompatibility and high in stability and can realize the functions of targeted transmission and monitoring tracking is prepared by adding amphiphilic p-sulfonated calix [4] arene in proper proportion on the basis that the liposome vesicle is prepared through the traditional phospholipid molecule and cholesterol. The preparation method disclosed by the invention has the advantages of easiness, convenience, small usage amount of main and object raw materials and wide application prospect in the field of targeted transportation of cancer medicines.

Description

【Technical field】 [0001] The invention belongs to the technical field of nano supramolecular materials, in particular to a novel multifunctional liposome vesicle and a preparation method thereof. 【Background technique】 [0002] In recent years, multifunctional vesicles have attracted much attention in the fields of biotechnology, medical diagnosis, and drug delivery. The main reason is that vesicles have a structure similar to cell membranes, and can contain hydrophilic molecules in their lumens and hydrophobic molecules in their hydrophobic membranes (J. Nicolas, S. Mura, D . Brambilla, N. Mackiewicz, P. Couvreur. Chem. Soc. Rev. 2013, 42, 1147–1235; (2) M. Elsabahy, K. L. Wooley. Chem. Soc. Rev. 2012, 41, 2545–2561.) . A common functionalization method is to link functional groups, such as targeting agents and fluorescent probes, to amphiphilic molecules that construct vesicles through covalent bonds. The resulting multifunctional vesicles can not only be used for biolog...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/22A61K47/26A61K49/00A61P35/00
Inventor 刘育王以轩郭东升王鲲鹏
Owner NANKAI UNIV
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