Method for massively preparing midkine antisense oligodeoxynucleotide nano-liposomes for injection

An antisense oligonucleotide and nanoliposome technology, applied in liposome delivery, gene therapy, pharmaceutical formulations, etc., can solve problems such as instability and difficulty in passing through cell membranes, and achieve the effect of improving curative effect
CN102989007AActive Publication Date: 2013-03-27HUZHOU CENT HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUZHOU CENT HOSPITAL
Publication Date
2013-03-27

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Abstract

The invention relates to a method for massively preparing midkine antisense oligodeoxynucleotide nano-liposomes for injection. The method comprises the following steps: dissolving a mixture of cationic liposomes and lecithoid liposomes in an organic solvent to obtain a liposome liquor; then, initially dispersing the liposomes by an ultrasonic instrument, and ultrafiltering to remove the organic solvent, and finally squeezing by a squeezer at a high pressure to uniform grain size to obtain blank nano-liposomes; and then, mixing with an MK-ASODN liquor to obtain MK-ASODN nano-liposomes. The grain size of the midkine antisense oligodeoxynucleotide nano-liposomes for injection produced by the method is within 100-500nm, and the midkine antisense oligodeoxynucleotide nano-liposomes for injection is stable in property and the anti-hepatoma effect of the midkine antisense oligodeoxynucleotide nano-liposomes for injection is remarkably enhanced. The method is simple in process, easy to realize, very easy to amplify, and suitable for industrial production.
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Description

[0001] technical field

[0002] The invention belongs to the technical field of preparation of anti-liver cancer drugs, and relates to a method for large-scale preparation of midkine antisense oligonucleotide nano-liposomes.

[0003] Background technique

[0004] Primary hepatocellular carcinoma (hepatocellular carcinoma, HCC) is a highly invasive, highly metastatic solid malignant tumor, but there is still a lack of ideal treatment.

[0005] Midkine (MK) is a newly discovered pro-angiogenic factor. Basic heparin-binding protein expressed only in mid-embryonic and adult kidneys. Existing studies have shown that MK mRNA and protein are highly expressed in many human tumor tissues such as liver cancer, while various normal tissues have only low or no expression, and are related to the occurrence, invasion and metastasis, angiogenesis and prognosis of liver cancer. are closely related, and angiogenesis is the basis for unrestricted invasion, growth and metastasis of liver ca...

Claims

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