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PLNPs as well as pharmaceutical composition and application thereof

A nanoparticle and liposome technology, applied in the field of medicine, can solve the problems of delivery, limited application, large size of Cas9 protein, etc., and achieve high transfection effect and good therapeutic effect

Inactive Publication Date: 2018-06-01
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large size of the commonly used Cas9 protein, it is difficult to efficiently deliver it with the current commonly used carriers, which limits its application in basic research and treatment

Method used

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  • PLNPs as well as pharmaceutical composition and application thereof
  • PLNPs as well as pharmaceutical composition and application thereof
  • PLNPs as well as pharmaceutical composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] This example is used to illustrate the preparation method of liposome nanoparticles provided by the present invention.

[0031] The molar ratio of liposomes, chondroitin sulfate and protamine is 0.2:4:4. The molar ratio of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride and cholesterol is 1:13:14.

[0032] Described preparation method is specifically as follows:

[0033] (1) According to the ratio, the liposomes are mixed with chondroitin sulfate to obtain the first mixed solution, and stand still for 15-30 minutes,

[0034] (2) According to the proportioning ratio, mix the first mixed solution obtained in step (1) with protamine to obtain the second mixed solution, and stand still for 15-30 minutes,

[0035] (3) According to the proportion, mix the second mixed solution obtained in step (2) with the liposome vesicles to obtain the third mixed solution, and stand still for 15-30 minutes,

[0036] (4) According to the proportion, mix the...

Embodiment 2～10

[0044] This example is used to illustrate the preparation method of liposome nanoparticles provided by the present invention.

[0045] The liposome nanoparticles were prepared by the preparation method described in Example 1.

[0046] Among them, the molar ratios of liposomes, chondroitin sulfate and protamine, and the molar ratios of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride and cholesterol are as follows:

[0047]

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Abstract

The invention provides PLNPs. The PLNPs are nanoparticles wrapped by liposome vesicles, and the surfaces of the liposome vesicles are modified with distearoyl phosphatidylethanolamine-PEG; the nanoparticles are prepared from lipid particles, chondroitin sulfate and protamine; the liposome vesicles are prepared from dioleoyl phosphatidylethanolamine, N-[1-(2,3-dioleyloxy) propyl]-N,N,N-trimethylammonium chloride and cholesterol. The invention further provides a preparation method of the PLNPs and pharmaceutical composition containing the PLNPs. The PLNPs as a carrier have higher transfection effect. The pharmaceutical composition has better treatment effect on various tumors.

Description

technical field [0001] The invention belongs to the technical field of medicine. Specifically, the present invention provides a liposome nanoparticle and its pharmaceutical composition and application. Background technique [0002] At present, in the gene therapy of various tumors, the commonly used vectors mainly include viral vectors and non-viral vectors. Although viral vectors exhibit excellent gene transfection efficiencies, they may induce mutagenesis and other oncogenic effects. Furthermore, repeated use of viral vectors may induce immunodeficiency. Commonly used non-viral vectors include polymers, nanoparticles (magnetic nanoparticles, gold nanoparticles, carbon nanoparticles with different surface modifications) and commercial reagents (Lipofectamine2000, Lipofectamine3000), etc., have low transfection efficiency and cannot play a role in cancer. to a good gene therapy effect. [0003] The CRISPR / Cas9 system is a versatile gene editing platform with potential ad...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/42A61K45/00A61P35/00
CPCA61K9/127A61K45/00A61K47/42
Inventor 蒋兴宇王鹏郑文富张灵敏
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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