PLNPs as well as pharmaceutical composition and application thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A nanoparticle and liposome technology, applied in the field of medicine, can solve the problems of delivery, limited application, large size of Cas9 protein, etc., and achieve high transfection effect and good therapeutic effect
Inactive Publication Date: 2018-06-01
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
View PDF3 Cites 3 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, due to the large size of the commonly used Cas9 protein, it is difficult to efficiently deliver it with the current commonly used carriers, which limits its application in basic research and treatment
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0030] This example is used to illustrate the preparation method of liposome nanoparticles provided by the present invention.
[0031] The molar ratio of liposomes, chondroitin sulfate and protamine is 0.2:4:4. The molar ratio of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride and cholesterol is 1:13:14.
[0032] Described preparation method is specifically as follows:
[0033] (1) According to the ratio, the liposomes are mixed with chondroitin sulfate to obtain the first mixed solution, and stand still for 15-30 minutes,
[0034] (2) According to the proportioning ratio, mix the first mixed solution obtained in step (1) with protamine to obtain the second mixed solution, and stand still for 15-30 minutes,
[0035] (3) According to the proportion, mix the second mixed solution obtained in step (2) with the liposome vesicles to obtain the third mixed solution, and stand still for 15-30 minutes,
[0036] (4) According to the proportion, mix the...
Embodiment 2～10
[0044] This example is used to illustrate the preparation method of liposome nanoparticles provided by the present invention.
[0045] The liposome nanoparticles were prepared by the preparation method described in Example 1.
[0046] Among them, the molar ratios of liposomes, chondroitin sulfate and protamine, and the molar ratios of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride and cholesterol are as follows:
[0047]
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention provides PLNPs. The PLNPs are nanoparticles wrapped by liposome vesicles, and the surfaces of the liposome vesicles are modified with distearoyl phosphatidylethanolamine-PEG; the nanoparticles are prepared from lipid particles, chondroitin sulfate and protamine; the liposome vesicles are prepared from dioleoyl phosphatidylethanolamine, N-[1-(2,3-dioleyloxy) propyl]-N,N,N-trimethylammonium chloride and cholesterol. The invention further provides a preparation method of the PLNPs and pharmaceutical composition containing the PLNPs. The PLNPs as a carrier have higher transfection effect. The pharmaceutical composition has better treatment effect on various tumors.
Description
technical field [0001] The invention belongs to the technical field of medicine. Specifically, the present invention provides a liposome nanoparticle and its pharmaceutical composition and application. Background technique [0002] At present, in the gene therapy of various tumors, the commonly used vectors mainly include viral vectors and non-viral vectors. Although viral vectors exhibit excellent gene transfection efficiencies, they may induce mutagenesis and other oncogenic effects. Furthermore, repeated use of viral vectors may induce immunodeficiency. Commonly used non-viral vectors include polymers, nanoparticles (magnetic nanoparticles, gold nanoparticles, carbon nanoparticles with different surface modifications) and commercial reagents (Lipofectamine2000, Lipofectamine3000), etc., have low transfection efficiency and cannot play a role in cancer. to a good gene therapy effect. [0003] The CRISPR / Cas9 system is a versatile gene editing platform with potential ad...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/42A61K45/00A61P35/00
CPCA61K9/127A61K45/00A61K47/42
Inventor 蒋兴宇王鹏郑文富张灵敏
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Login to view more 
Login to view more