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Antiviral small nucleic acid and temperature-sensitive type gel preparation and application thereof

A temperature-sensitive, gel preparation technology, applied in the directions of antiviral agents, antitumor drugs, aerosol delivery, etc., can solve the problem that the cervical precancerous disease cannot be effectively removed from HPV infection, and the protection rate is less than 60% and insufficient. Eliminate problems such as viruses, and achieve the effects of high mechanical strength, convenient administration and enhanced stability

Active Publication Date: 2014-06-11
YOUJIA (HANGZHOU) BIOMEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Humoral and cellular immunity induced by HSV can affect the severity of recurrent disease, but it is not sufficient to clear the virus. Currently developed vaccines are not protective against HSV-2 infection, while the protection rate against HSV-1 is less than 60%. Current treatments for HSV infection Treatment with oral nucleoside drugs, such as acyclovir and famciclovir, can inhibit the synthesis of viruses, but may cause adverse reactions in the nervous system, digestive system and the whole body
Effective control of HPV infection, timely detection and treatment of cervical precancerous lesions are the key to reducing the incidence and mortality of cervical cancer, but there is no drug that can effectively clear HPV infection and treat cervical precancerous lesions

Method used

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  • Antiviral small nucleic acid and temperature-sensitive type gel preparation and application thereof
  • Antiviral small nucleic acid and temperature-sensitive type gel preparation and application thereof
  • Antiviral small nucleic acid and temperature-sensitive type gel preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1, the preparation of small nucleic acid of the present invention

[0046] The small nucleic acid of the present invention is artificially synthesized, and the sequence is as follows:

[0047] siHPV16E7: its sense strand sequence is sequence 1 (5'CUACUGUUAUGAGCAAUUATT3') in the sequence listing, and its antisense strand sequence is sequence 2 (5'UAAUUGCUCAUAACAGUAGTT3') in the sequence listing; the double-stranded nucleic acid uses 2 bases on U and C 'Methoxy modification.

[0048] siHPV18E7: its positive sense strand sequence is sequence 3 (5'CCUUCUAUGUCACGAGCAATT3') in the sequence listing, and its antisense strand sequence is sequence 4 (5'UUGCUCGUGACAUAGAAGGTT3') in the sequence listing; the double-stranded nucleic acid uses 2 'Methoxy modification.

[0049] siB2M: its sense strand sequence is sequence 5 (5'GAAUGGAGAGAGAAUUGAA3') in the sequence listing, and its antisense strand sequence is sequence 6 (5'UUCAAUUCUCUCUCCAUUC3') in the sequence listing; t...

Embodiment 2

[0053] Embodiment 2, preparation of temperature-sensitive gel and its effect detection

[0054] 1. Preparation of thermosensitive gel

[0055] Components and concentrations of thermosensitive gels:

[0056]

[0057] 2. Prepare small nucleic acid temperature-sensitive gels according to the above-mentioned component concentrations, and the specific methods are as follows:

[0058] 1) Weigh 170.0g of Poloxamer 407 and 2.0g of hydroxypropyl methylcellulose (HPMC E-50lv, Shanghai Colorcon Company, Lot No: TK16012401), add 500ml of distilled water (about 4°C), and stir for 5 minutes , placed at 4°C to fully swell, disperse evenly, and dissolve to obtain a clear solution of a hydrophilic gel.

[0059] 2) Take 5.0g of collagen (purchased from Hubei Kangbaotai Fine Chemical Co., Ltd.), add 50ml of distilled water, stir at room temperature for 5min, and place it for use.

[0060] 3) Take 10.0g HP-β-CD and add 100ml distilled water to dissolve, heat to 50℃ to swell and wait for use...

Embodiment 3

[0064] Example 3, Preparation of temperature-sensitive gel and its effect detection

[0065] 1. Preparation of temperature-sensitive gel

[0066] Components of temperature-sensitive gels and their concentrations:

[0067]

[0068] 2. Prepare the above-mentioned component concentration temperature-sensitive gel, the specific method is as follows:

[0069] 1) Weigh 170.0g of Poloxamer 407 and 5.0g of methylcellulose, add 500ml of distilled water (at about 4°C), stir for 5 minutes, and place it at 4°C to fully swell, disperse evenly, and dissolve to obtain hydrophilic Clear solution of gel.

[0070] 2) Add 5.0g of collagen to 50ml of distilled water, stir at room temperature for 5min, and place it for use.

[0071] 3) Take 20.0g HP-β-CD and add 200ml distilled water to dissolve, heat to 50℃ to swell and wait for use.

[0072]4) Add 207.5g of 1,2-propanediol into the gel solution prepared in step 1) and stir to mix evenly. While stirring, slowly add the solutions in steps 2...

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Abstract

The invention discloses an antiviral small nucleic acid and the application of the small nucleic acid in preparing a medicine for suppressing herpes simplex virus (HSV) and human papilloma virus (HPV). The small nucleic acid is a double strand nucleic acid, the positive-sense strand sequence is 5 in a sequence table, and the antisense strand sequence is 6 in the sequence table; the small nucleic acid can be prepared into a temperature-sensitive type gel preparation; the temperature-sensitive type gel preparation is injected (or sprayed) into irregular cavities such as vagina or mouth, the expression of host B2M genes in epithelial cells at the cavities and the cervix uteri and the expression of externalHPV E7 are suppressed, so that the antiviral small nucleic acid can be used for suppressing the infection of the HPV of the HSV-1 virus and / or for treating the precancerous lesions of uterine cervix caused by the HVP.

Description

technical field [0001] The invention relates to a small nucleic acid and its temperature-sensitive gel preparation and their application in preparing medicines for inhibiting diseases caused by herpes simplex virus (HSV) and human papillomavirus (HPV). Background technique [0002] Herpes simplex virus (HSV) infection is a common cause of oral and genital herpes and ulcers. The population is generally susceptible to HSV. The infection rate of HSV-1 reaches about 80-90%, and the infection rate of HSV-2 is also as high as 22%-40%. HSV-1 mainly causes stomatitis and lip herpes, while HSV-2 is mostly transmitted through sexual activities and causes genital herpes. More and more studies have found that HSV-1 can also cause genital diseases, and it is more likely to cause genital diseases common cause. The virus can remain dormant in ganglia near the site of infection and remain virulent for life. Intermittent symptomatic activation causes distress to the patient, while asympto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113A61K48/00A61K9/06A61P35/00A61P31/20A61P31/22
Inventor 张雅鸥王纠何杰谢伟东许乃寒李颖张佩琢苏宏瑞万刚吕青卢锦华柳忠义
Owner YOUJIA (HANGZHOU) BIOMEDICAL TECH CO LTD
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