This invention relates to the field of
biomedicine, specifically to a method for preparing and applying a biomimetic
lipoprotein nanodisk to enhance the
therapeutic effect on tumors. The method for preparing the biomimetic
lipoprotein nanodisk provided by this invention includes the following steps: First, CCL5
peptide and DSPE-PEG2000-mal are dissolved in DMF and
triethylamine, stirred at
room temperature, and then the reaction solution is purified by
dialysis and freeze-dried to obtain a white
solid powder; then,
methanol is placed in a flask, DPPC, DSPE-PEG2000-CCL5, and NG are dissolved in
methanol,
acetic acid is added, and the mixture is evaporated to
dryness to form a thin film in the flask; then, the film is dispersed with
ultrapure water, sonicated with a probe in an ice bath, and then ApoA1
peptide is added to the
mixed solution, followed by thermal
cycling to obtain C-LNG. This invention optimizes and modifies
gemcitabine, developing a new
drug NG, which significantly improves the
therapeutic effect on tumors, and C-LNG has extremely high targeting and
drug loading rates, further enhancing the
drug's
efficacy.