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Magnetic transfection reagent based on supramolecule self-assembly

A supramolecular self-assembly and transfection reagent technology, applied in recombinant DNA technology, other methods of inserting foreign genetic materials, etc., can solve problems such as cytotoxicity, and achieve the effects of good repeatability, simple reaction conditions, and mature conditions for exploration.

Inactive Publication Date: 2016-02-03
NANKAI UNIV
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Problems solved by technology

However, from the research results of nanotoxicology, most nanomaterials have different degrees of cytotoxicity.

Method used

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  • Magnetic transfection reagent based on supramolecule self-assembly
  • Magnetic transfection reagent based on supramolecule self-assembly
  • Magnetic transfection reagent based on supramolecule self-assembly

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Experimental program
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Embodiment

[0025] A magnetic transfection reagent based on supramolecular self-assembly, which is formed by assembling magnetic balls made by pyrolysis and 6-position fully substituted α-cyclodextrin-PEI600 (CD-PEI) through self-assembly, 6-position fully substituted The structural formula of α-cyclodextrin-PEI600 is as follows:

[0026]

[0027] A preparation method of a magnetic transfection reagent based on supramolecular self-assembly, the steps are as follows:

[0028] 1) Synthesis of α-cyclodextrin-PEI600 fully substituted at the 6-position of the assembly motif

[0029] Activate α-cyclodextrin with N,N-carbonyldiimidazole (CDI), dissolve 0.973g α-cyclodextrin in 10mL dimethyl sulfoxide (DMSO), dissolve 1.297g N,N-carbonyldiimidazole in di In methyl sulfoxide, the α-cyclodextrin solution was slowly added dropwise to the N,N-carbonyldiimidazole solution, reacted for 3 hours under the protection of nitrogen, and the obtained product was precipitated in ether to obtain the interme...

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Abstract

The invention relates to a magnetic transfection reagent based on supramolecule self-assembly, wherein magnetic spheres prepared through a high temperature decomposition method and six-site fully-substituted alpha-cyclodextrin-PEI600 (CD-PEI) are assembled through self-assembly to form the magnetic transfection reagent. According to the present invention, the preparation method of the magnetic transfection reagent is provided, the structure of the magnetic transfection reagent is characterized, and the biological toxicity and the transfection performance of the magnetic transfection reagent at the cell level are characterized; the preparation method is mild, simple and green environmental protection; and the synthesized magnetic transfection reagent has characteristics of smaller particle size, surface having a lot of positive charges, good siRNA combination ability, low biological toxicity, high transfection efficiency, and good application prospects, and the performance of the magnetic transfection reagent as the siRNA vector is excellent.

Description

technical field [0001] The invention relates to the research fields of polymer chemistry, biomedical materials, magnetic materials and nanotechnology, in particular to a magnetic transfection reagent based on supramolecular self-assembly. Background technique [0002] RNA interference (RNAi) has great application potential in the targeted treatment of diseases such as tumors, genetic defects, and diabetes. Although the mechanism of RNAi has been clarified, its clinical application has been greatly limited due to the lack of effective delivery vectors for siRNA. Although RNAi based on viral vectors can have both targeting and high transfection efficiency, the application of viral vectors may also cause certain risks. Nanomaterials are a typical class of non-viral vectors, which generally have the advantages of small size and easy modification, and can effectively mediate siRNA into cells and induce RNAi effects. However, according to the research results of nanotoxicology, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C12N15/87
Inventor 王燕铭王鹏杨苏崔冬董博
Owner NANKAI UNIV
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