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A preparation method of nano-sulfonated graphene and application of nano-sulfonated graphene as gene transfer material

A technology of sulfonated graphene and gene introduction, which is applied in the directions of graphene, chemical instruments and methods, nano-carbon, etc., can solve the problems of low transfection efficiency, limited penetration of gene guns, and high production cost, and achieves a simple preparation method. , good repeatability and low production cost

Active Publication Date: 2015-08-26
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Abstract
  • Description
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Problems solved by technology

Therefore, the non-viral gene transfer method is a current research focus, but the above-mentioned non-viral gene transfer methods all have shortcomings: microinjection can only process one cell at a time, and its transfection efficiency is very low; Penetration is very limited; the transfection efficiency of the calcium phosphate co-precipitation method is affected by many factors such as temperature, concentration, and operating environment, and the transfection result is very unstable; although the cationic liposome method shows good transfection efficiency, the cationic liposome method Due to the high toxicity of liposomes, the application of the cationic liposome method is limited; the nano-gene transfer materials in the prior art have the disadvantages of high production cost, difficult availability of raw materials for preparation, and difficulty in popularization and application

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  • A preparation method of nano-sulfonated graphene and application of nano-sulfonated graphene as gene transfer material
  • A preparation method of nano-sulfonated graphene and application of nano-sulfonated graphene as gene transfer material
  • A preparation method of nano-sulfonated graphene and application of nano-sulfonated graphene as gene transfer material

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Embodiment 1

[0045] A kind of preparation method of nanometer sulfonated graphene, it comprises the following steps:

[0046] Step 1, prepare graphene oxide by Hummers method: prepare graphene oxide by Hummers method with graphite powder, potassium permanganate, sodium nitrate and concentrated sulfuric acid;

[0047] Step 2, the preparation of reduced graphene oxide:

[0048] (1) Disperse graphene oxide in deionized water, and then perform ultrasonic treatment for a certain period of time to obtain a graphene oxide dispersion system; wherein, the mass ratio of deionized water to graphene oxide is 1000:1;

[0049](2) adding sodium carbonate solution to the dispersion system of graphene oxide, so that the pH value of the dispersion system of graphene oxide is 9;

[0050] (3) Add sodium borohydride solution to the above-mentioned graphene oxide dispersion system with a pH value of 9, and then stir at 80°C for 1 hour to obtain reduced graphene oxide, and then wash the reduced graphene oxide w...

Embodiment 2

[0060] A kind of preparation method of nanometer sulfonated graphene, it comprises the following steps:

[0061] Step 1, prepare graphene oxide by Hummers method: prepare graphene oxide by Hummers method with graphite powder, potassium permanganate, sodium nitrate and concentrated sulfuric acid;

[0062] Step 2, the preparation of reduced graphene oxide:

[0063] (1) Disperse graphene oxide in deionized water, and then perform ultrasonic treatment for a certain period of time to obtain a graphene oxide dispersion system; wherein, the mass ratio of deionized water to graphene oxide is 980:1;

[0064] (2) adding sodium carbonate solution to the dispersion system of graphene oxide, so that the pH value of the dispersion system of graphene oxide is 10;

[0065] (3) Add sodium borohydride solution to the above-mentioned graphene oxide dispersion system with a pH value of 10, and then stir at 75°C for 1.2 hours to obtain reduced graphene oxide, and then wash the reduced graphene ox...

Embodiment 3

[0075] A kind of preparation method of nanometer sulfonated graphene, it comprises the following steps:

[0076] Step 1, prepare graphene oxide by Hummers method: prepare graphene oxide by Hummers method with graphite powder, potassium permanganate, sodium nitrate and concentrated sulfuric acid;

[0077] Step 2, the preparation of reduced graphene oxide:

[0078] (1) Disperse graphene oxide in deionized water, and then perform ultrasonic treatment for a certain period of time to obtain a graphene oxide dispersion system; wherein, the mass ratio of deionized water to graphene oxide is 1020:1;

[0079] (2) adding sodium carbonate solution to the dispersion system of graphene oxide, so that the pH value of the dispersion system of graphene oxide is 9.5;

[0080] (3) Add sodium borohydride solution to the above-mentioned graphene oxide dispersion system with a pH value of 9.5, and then stir at 85°C for 0.8 hours to obtain reduced graphene oxide, and then wash the reduced graphene...

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Abstract

The invention relates to the technical field of gene transfer materials, particularly a preparation method of nano sulfonated graphene and application of nano sulfonated graphene as a gene transfer material. The preparation method of the nano sulfonated graphene comprises the following steps: 1. preparing graphene oxide; 2. preparing reduced graphene oxide; 3. preparing diazonium salt of sulfanilic acid; and 4. preparing the nano sulfonated graphene. The nano sulfonated graphene can be used as a gene transfer material since positive charges of ions on particle surfaces can be combined with green fluorescent proteins in a non-covalent mode to perform transfection; and when being used as a gene transfer material, the nano sulfonated graphene has the advantages of high transfection effect, favorable cell compatibility and high cell survival rate. The transfection efficiency of the nano sulfonated graphene in human breast cancer cells is up to 40% or so.

Description

technical field [0001] The invention relates to the technical field of gene transfer materials, in particular to a preparation method of nano-sulfonated graphene and the application of nano-sulfonated graphene as a gene transfer material. Background technique [0002] Gene delivery methods can be divided into two categories: the first category is viral gene delivery methods, which use retroviruses, adenoviruses, and adeno-associated viruses as carriers; the second category is non-viral gene delivery methods, such as microinjection, gene Gun, calcium phosphate co-precipitation, cationic liposome method, and the use of emerging nano-gene delivery materials for gene transfection. [0003] Viral gene transfer methods have many serious disadvantages, such as the possibility of activation of proto-oncogenes during viral transfection. Therefore, the non-viral gene transfer method is a current research focus, but the above-mentioned non-viral gene transfer methods all have shortcom...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B31/04A61K47/04A61K48/00A61P35/00C01B32/184C01B32/194C01B32/198
Inventor 王深明徐安武张德元常光其周鸿雁李梓伦夏浩明张红霞
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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