SiRNA-loaded bionic ZIF-8 nanometer transmission system, and preparation method and application of siRNA-loaded bionic ZIF-8 nanometer transmission system

A technology of ZIF-8 and delivery system, applied in the field of biomedicine, can solve problems such as difficult maintenance of blood drug concentration, delivery of macromolecules, limitation of cancer treatment effect, etc., and achieve high transfection effect and good therapeutic effect

Inactive Publication Date: 2021-12-14
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation scale of the existing technology is small, and the chemical drugs have high resistance and non-specific distribution. It is difficult to deliver macromolecules (such as nucleic acids), and it is difficult to maintain the blood drug concentration for a long time, which limits the therapeutic effect of cancer.

Method used

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  • SiRNA-loaded bionic ZIF-8 nanometer transmission system, and preparation method and application of siRNA-loaded bionic ZIF-8 nanometer transmission system
  • SiRNA-loaded bionic ZIF-8 nanometer transmission system, and preparation method and application of siRNA-loaded bionic ZIF-8 nanometer transmission system
  • SiRNA-loaded bionic ZIF-8 nanometer transmission system, and preparation method and application of siRNA-loaded bionic ZIF-8 nanometer transmission system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] This example is used to illustrate the method for constructing biofilm-wrapped nanoparticles using nanomaterials and extracted Hela cell membranes.

[0057] 1) Dissolve siPlk1 dry powder in nuclease-free water to obtain 20 μM siPlk1 solution;

[0058] 2) Take dimethylimidazole (MIM, 231.43 μM)) and the above dissolved siPlk1 solution and incubate at room temperature for 10 min;

[0059] 3) Take 3.36μM zinc nitrate hexahydrate (Zn(NO3) 2 ·6H 2 O)(mass ratio Zn(NO3) 2 ·6H 2 (0:MIM=1 / 19) was added to the above solution under stirring, and incubated at room temperature for 10 min;

[0060] 4) Prepare MOFP with different weight ratios (MOF:siRNA) such as 50, 100, 200, 400, 800, 1600 and 3200);

[0061] 5) The above mixture was centrifuged at 4°C and 12000rpm for 15 minutes, the supernatant was removed, and then washed twice, and the obtained precipitate was resuspended in sterile double distilled water;

[0062] 6) The above mixture was mixed with Hela cell membrane, a...

Embodiment 2

[0077] This example is used to illustrate that the method of the present invention can be prepared on a large scale.

[0078] Ratio of siRNA to ZIF-8 from 1:3200, 1:1600, 1:800, 1:400, 1:200, 1:100 to 1:50 ( figure 1 C), it can be seen that when the ratio of material to siRNA reaches 1:200, the drug loading rate still reaches 94%.

[0079] 1) Dissolve 50 mg of siPLK1 dry powder in nuclease-free water to obtain a 20 μM siPLK1 solution;

[0080] 2) Take 153.01 μM dimethylimidazole (MIM) and 20 μM dissolved siLuciferase solution and incubate at room temperature for 10 min;

[0081] 3) Take 2.18 μM zinc nitrate hexahydrate (Zn(NO 3 ) 2 6H2O) was added to the above solution under stirring, and incubated at room temperature for 10 minutes;

[0082] 4) The above mixture was centrifuged at 4°C and 12000rpm for 15 minutes, the supernatant was removed, and then washed twice, and the obtained precipitate was resuspended in sterile double distilled water;

[0083] 5) Mix the above mi...

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Abstract

The invention provides an SiRNA-loaded bionic ZIF-8 nanometer transmission system, and a preparation method and application of the siRNA-loaded bionic ZIF-8 nanometer transmission system. The nanometer transmission system comprises ZIF-8 and siRNA wrapped by a cell membrane. The nanometer transmission system has a relatively high transfection effect, has a relatively good treatment effect for tumor inhibition, and can be prepared on a large scale.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a biomimetic ZIF-8 nano delivery system loaded with siRNA, its preparation method and application. Background technique [0002] Polo-like kinase I (polo-like kinase, PLK1) is a serine / threonine protein kinase that plays an important role in the cell cycle. PLK1 plays a key role in the process of cell division and differentiation. PLK-1 is overexpressed in most cancer cells and is closely related to the occurrence and prognosis of tumors. Therefore, it is considered to be an important target of antitumor drugs. Clinical findings show that cervical cancer, ovarian cancer, and breast cancer are highly resistant to chemotherapy due to lack of specificity. At the same time, studies have shown that the expression of PLK1 in cervical cancer is significantly higher than that in normal cells, and inhibiting the expression of PLK1 can significantly inhibit tumor growth. Growth, but...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K31/713A61K47/54A61K47/69A61P35/00
CPCA61K9/5068A61K31/713A61K47/6949A61K47/6929A61K47/545A61P35/00
Inventor 张灵敏余细勇张燕芬林忠晓
Owner GUANGZHOU MEDICAL UNIV
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