Gold nanocluster-liposome composite nanoparticles, preparation method and applications thereof

A technology of gold nanoclusters and composite nanoparticles, applied in the field of gold nanocluster-liposome composite nanoparticles and its preparation, can solve the problems of delivery, large size of Cas9 protein, limited application, etc., achieve high transfection effect, improve Cell uptake rate and transfection efficiency, effect of efficient delivery

Active Publication Date: 2018-09-07
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the large size of the commonly used Cas9 protein, it is difficult to efficiently deliver

Method used

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  • Gold nanocluster-liposome composite nanoparticles, preparation method and applications thereof
  • Gold nanocluster-liposome composite nanoparticles, preparation method and applications thereof
  • Gold nanocluster-liposome composite nanoparticles, preparation method and applications thereof

Examples

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Embodiment 1

[0028] This example is used to illustrate the preparation method of the gold nanocluster-liposome composite nanoparticle provided by the present invention.

[0029] 1. Preparation of membrane-penetrating peptide-modified gold nanoclusters:

[0030] (1) Mix 300 μl of chloroauric acid with a concentration of 100 mM, 10 ml of glutathione with a concentration of 2 mM and 10 ml of penetrating peptide with a concentration of 0.5 mM to obtain a mixed solution.

[0031] (2) Heating the mixture in step (1) at 50° C. for 12 hours to obtain gold nanoclusters modified with membrane-penetrating peptides.

[0032] The particle size of the gold nanocluster is less than 5nm.

[0033] 2. Preparation of gold nanocluster-liposome composite nanoparticles

[0034] The molar ratio of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride and cholesterol is 1:0.8:2.

[0035] Described preparation method is specifically as follows:

[0036] (1) According to the ratio, mix ...

Embodiment 2～10

[0046] This example is used to illustrate the preparation method of the gold nanocluster-liposome composite nanoparticle provided by the present invention.

[0047] The preparation method described in Example 1 was used to prepare the gold nanocluster-liposome composite nanoparticles.

[0048] Among them, the molar ratios of gold nanoclusters, Cas9 protein, and guide ribonucleic acid, and the molar ratios of dioleoylphosphatidylethanolamine, 2,3-dioleoxypropyltrimethylammonium chloride, and cholesterol are as follows:

[0049]

[0050]

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Abstract

The present invention provides gold nanocluster-liposome composite nanoparticles, which are a gold nanocluster encapsulated with a liposome vesicle, wherein the surface of the liposome vesicle is modified with distearoyl phosphatidylethanolamine-polyethylene glycol, the surface of the gold particle in the gold nanocluster is modified with a penetrating peptide and is bound to Cas9 protein and guiding ribonucleic acid, and the liposome vesicle comprises dioleoyl phosphoethanolamine, 2,3-dioleyloxypropyltrimethyl ammonium chloride and cholesterol. The invention further provides a preparation method and applications of the gold nanocluster-liposome composite nanoparticles. According to the present invention, by using the gold nanocluster-liposome composite nanoparticles, the CRISPR/Cas9 system can be efficiently delivered into cells without any other transfection reagents so as to improve the cell intake rate and the transfection efficiency, such that the gene treatment effects on a variety of tumors can be achieved, and the tracing effect can be achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine. Specifically, the present invention provides a gold nanocluster-liposome composite nanoparticle and its preparation method and application. Background technique [0002] At present, in the gene therapy of various tumors, the commonly used vectors mainly include viral vectors and non-viral vectors. Although viral vectors exhibit excellent gene transfection efficiencies, they can induce mutagenesis and other oncogenic effects. Furthermore, repeated use of viral vectors may induce immunodeficiency. Commonly used non-viral vectors include polymers, nanoparticles (magnetic nanoparticles, gold nanoparticles, carbon nanoparticles with different surface modifications) and commercial reagents (Lipofectamine2000, Lipofectamine3000), etc., have low transfection efficiency and cannot play a role in cancer. to a good gene therapy effect. [0003] The CRISPR / Cas9 system is a versatile gene editing platfor...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K47/24A61K47/02A61K48/00A61K38/46A61P35/00C12N15/87
CPCA61K38/46A61K47/02A61K47/24A61K47/42A61K9/1271C12N15/87C12N2800/107C12N2800/80C12N2810/10
Inventor 蒋兴宇王鹏郑文富张灵敏
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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