Human umbilical cord MSC (mesenchymal stem cell) serum-free medium liposome freeze-dried powder as well as preparation method and application thereof

A serum-free culture medium, human umbilical cord technology, applied in cosmetic preparations, cosmetic preparations, preparations for skin care, etc., can solve the problems of short shelf life, immune rejection, poor compatibility of cosmetics, etc. Solve the effect of short shelf life and reduce immune rejection

Active Publication Date: 2016-12-07
西安艾尔菲生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] An object of the present invention is to provide a liposome freeze-dried powder of human umbilical cord mesenchymal stem cell serum-free culture solution, which solves the problem that the existing human umbilical cord mesenchymal stem cell culture supernatant is easy to cause immune rejection and has a short shelf life. And the problem of p

Method used

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  • Human umbilical cord MSC (mesenchymal stem cell) serum-free medium liposome freeze-dried powder as well as preparation method and application thereof
  • Human umbilical cord MSC (mesenchymal stem cell) serum-free medium liposome freeze-dried powder as well as preparation method and application thereof
  • Human umbilical cord MSC (mesenchymal stem cell) serum-free medium liposome freeze-dried powder as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0039] Prepare a kind of human umbilical cord mesenchymal stem cell serum-free culture fluid liposome freeze-dried powder, its method is as follows:

[0040] Step (1), preparing human umbilical cord mesenchymal stem cell serum-free culture medium

[0041] Extraction of human umbilical cord mesenchymal stem cells: Under sterile conditions, take 5-10 cm of neonatal umbilical cord, put it in umbilical cord preservation solution, and store it at 4°C (for no more than 8 hours). Flush with D-Hanks solution until there is no blood in the vein. Remove the epidermis, two arteries and one vein, and cut the tissue to about 1mm 3 / block, add DMEM medium (containing double antibody) containing 10% FBS and culture for about a week, the culture conditions are 37°C, saturated humidity, 5% CO 2 , to obtain primary human umbilical cord mesenchymal stem cells.

[0042] Collect serum-free culture medium: when the cell confluency is 80%, trypsinize the cells for subculture, and take the 6th pa...

Embodiment 2

[0050] Prepare a kind of human umbilical cord mesenchymal stem cell serum-free culture fluid liposome freeze-dried powder, its method is as follows:

[0051] Step (1), preparing human umbilical cord mesenchymal stem cell serum-free culture medium

[0052] Extraction of human umbilical cord mesenchymal stem cells: Under sterile conditions, take 5-10 cm of neonatal umbilical cord, put it in umbilical cord preservation solution, and store it at 4°C (for no more than 8 hours). Flush with D-Hanks solution until there is no blood in the vein. Remove the epidermis, two arteries and one vein, and cut the tissue to about 1mm 3 / block, add DMEM medium (containing double antibody) containing 10% FBS and culture for about a week, the culture conditions are 37°C, saturated humidity, 5% CO 2 , to obtain primary human umbilical cord mesenchymal stem cells.

[0053] Collect serum-free culture medium: when the cell confluency is 90%, trypsinize the cells for subculture, and take the 10th p...

Embodiment 3

[0061] Prepare a kind of human umbilical cord mesenchymal stem cell serum-free culture fluid liposome freeze-dried powder, its method is as follows:

[0062] Step (1), preparing human umbilical cord mesenchymal stem cell serum-free culture medium

[0063] Extraction of human umbilical cord mesenchymal stem cells: Under sterile conditions, take 5-10 cm of neonatal umbilical cord, put it in umbilical cord preservation solution, and store it at 4°C (for no more than 8 hours). Flush with D-Hanks solution until there is no blood in the vein. Remove the epidermis, two arteries and one vein, and cut the tissue to about 1mm 3 / block, add F12 medium (containing double antibody) containing 10% FBS and culture for about a week, the culture conditions are 37°C, saturated humidity, 5% CO 2, to obtain primary human umbilical cord mesenchymal stem cells.

[0064] Collect serum-free culture medium: when the cell confluency is 85%, trypsinize the cells for subculture, and take the 15th pas...

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Abstract

The invention discloses human umbilical cord MSC (mesenchymal stem cell) serum-free medium liposome freeze-dried powder, which consists of 1-3% of a human umbilical cord MSC serum-free medium active protein ingredient, 15-60% of phospholipid, 1-12% of cholesterol, 1-25% of Tween-80, 0.5-10% of a lipid-soluble antioxidant, 10-60% of a cryoprotectant and 1-5% of water. The invention also discloses a preparation method of the liposome freeze-dried powder, wherein the preparation method comprises the following steps: firstly, dissolving the phospholipid, the cholesterol, the Tween-80 and the lipid-soluble antioxidant in absolute ethyl alcohol, so that a raw material solution is obtained; then, adding the raw material solution to a buffer solution which contains the cryoprotectant and conducting hydrating; after conducting hydrating, removing all ethanol, and conducting ultrasonic granulation, so that a blank liposome suspension is obtained; and conducting an encapsulation reaction by adding a human umbilical cord MSC serum-free medium to the blank liposome suspension, conducting film filtration and granulation on a finished product, and sequentially implementing freeze-thawing and freeze-drying, so that the liposome freeze-dried powder is obtained. The liposome freeze-dried powder disclosed by the invention, which is directly added to cosmetics after being hydrated, has a function of delaying aging. The liposome freeze-dried powder disclosed by the invention is long in shelf life, good in cosmetic effect and free from immunological rejection.

Description

technical field [0001] The invention belongs to the technical field of stem cell culture liquid cosmetics, in particular to a human umbilical cord MSC (mesenchymal stem cell) serum-free culture liquid liposome freeze-dried powder, and also relates to the preparation of the stem cell serum-free culture liquid liposome freeze-dried powder Preparation methods and applications. Background technique [0002] Stem cell cosmetology was first applied in plastic surgery. The types of stem cells used more in plastic surgery are mesenchymal stem cells, epidermal stem cells, vascular endothelial progenitor cells, and preadipocytes. [0003] Human mesenchymal stem cells (MSC) secrete basic fibroblast growth factor (bFGF), human epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF) in culture -1) and hepatocyte growth factor (HGF), a variety of growth factors that promote skin cell regeneration. These biologically active growth fact...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/67A61K8/63A61K8/55A61K8/49A61K8/35A61K8/33A61K8/14A61Q19/08
CPCA61K8/14A61K8/33A61K8/355A61K8/4993A61K8/553A61K8/63A61K8/678A61K8/99A61K2800/72A61K2800/84A61Q19/08
Inventor 王静胡春梅刘艳赵文静
Owner 西安艾尔菲生物科技有限公司
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