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Polymer coated vitamin E liposome and preparation method thereof

A technology of vitamins and polymers, which is applied in liposome delivery, food preparation, non-active ingredients of polymer compounds, etc. It can solve problems such as product packaging, storage and transportation difficulties, drug leakage stability, and particle size increase. , to achieve the effects of convenient packaging, prolonging the action time and improving stability

Inactive Publication Date: 2010-07-21
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The non-encapsulated liposomes prepared by the above-mentioned background technology have poor stability problems such as flocculation, particle size gradually increasing, and drug leakage after being placed for a certain period of time, and the product has certain problems in packaging, storage and transportation. difficulty

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take vitamin E 0.5g, egg yolk lecithin 2.5g, cholesterol 1g, Tween 60 0.1g, Span 80 0.25g, TBHQ 0.05g, ether 20ml, polyethylene glycol 4000 3g, pH6.8 phosphate buffer solution 200ml.

[0022] Dissolve vitamin E, egg yolk lecithin, cholesterol, Tween 60, Span 80, and TBHQ in the above prescription with ether, use a rotary evaporator to remove the ether (30-60°C, vacuum degree 0.2-0.3MPa), and wait for the ether to volatilize After finishing, stop rotating and keep the vacuum and continue to suck after 30min, stop, add the phosphate buffer solution 100ml of pH6.8, after being placed on the shaker and shake for 40min, sonicate under ice-water bath condition (ultrasonic power 80%, sonication time 8min ), to obtain vitamin E liposome suspension. Take another 100ml of phosphate buffer solution dissolved with polyethylene glycol 4000PH6.8, pump the above suspension into the phosphate buffer solution with a constant flow pump at a flow rate of 2ml / min, and magnetically stir the...

Embodiment 2

[0024] Take vitamin E 0.8g, soybean lecithin 5g, cholesterol 1.2g, Tween 60 0.18g, polyoxyethylene castor oil 0.1g, V C Palmitate 0.08g, dichloromethane 20ml, cyclodextrin 4.5g, pH6.8 phosphate buffer solution 200ml.

[0025] Vitamin E, soybean lecithin, cholesterol, Tween 60, polyoxyethylene castor oil, V C Dissolve palmitate in dichloromethane, use a rotary evaporator to remove the dichloromethane (30-50°C, vacuum degree 0.2-0.3MPa), after the dichloromethane has evaporated, stop the rotation and keep the vacuum for 30 minutes and then stop , adding 100 ml of pH6.8 phosphate buffer solution, placed on a shaker and shaken for 40 minutes, and then ultrasonicated in an ice-water bath (80% ultrasonic power, 8 min ultrasonic time) to obtain a suspension. Take another 100ml of phosphate buffer solution with pH6.8 dissolved in cyclodextrin, pump the above suspension into the phosphate buffer solution with a constant flow pump at a flow rate of 2ml / min, and magnetically stir the so...

Embodiment 3

[0027] Take vitamin E 0.75g, soybean lecithin 4.5g, cholesterol 1.2g, Benzene 0.12g, polyoxyethylene castor oil 0.1g, TBHQ 0.05g, dichloromethane 20ml, polyvinyl alcohol 3.5g, pH6.8 phosphate buffer solution 200ml.

[0028]Dissolve vitamin E, soybean lecithin, cholesterol, benzze, polyoxyethylene castor oil, and TBHQ in the above prescription with dichloromethane, and use a rotary evaporator to remove the dichloromethane (30-50°C, vacuum degree 0.2-0.3MPa ), after the volatilization of dichloromethane, stop the rotation and keep the vacuum and continue to suck for 30min, then stop, add 100ml of pH6. 80%, ultrasonic time 8min) to obtain a suspension. Take another 100ml of phosphate buffer solution dissolved in polyvinyl alcohol pH6.8, pump the above suspension into the phosphate buffer solution with a constant flow pump at a flow rate of 2ml / min, and magnetically stir the solution under the condition of a constant temperature water bath at 25°C. After the liposome suspension ...

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PUM

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Abstract

The invention relates to a polymer coated vitamin E liposome and a preparation method thereof. The preparation method of the invention comprises the following steps: preparing a vitamin E liposome suspension; adding a polymer into the suspension for coating to obtain a polymer coated vitamin E liposome suspension; obtaining a polymer coated vitamin E proliposome solid powder by spray drying or freeze drying; and before use, adding a proper amount of distilled water as required to obtain the polymer coated vitamin E liposome suspension with the entrapment rate of 82-94%. The polymer coated vitamin E liposome of the invention can obviously reduce the degree of oxidation of vitamin E, reduce the percolation rate, delay the release of the vitamin E in vivo, prolong the cycling time in vivo, and be prepared into the coated type proliposome solid powder which can be more conveniently packed, stored, transported and used.

Description

technical field [0001] The invention relates to the field of functional health food, in particular to a polymer-coated vitamin E liposome and a preparation method thereof. Background technique [0002] Vitamin E is a fat-soluble natural antioxidant. Vitamin E can reduce the level of reactive oxygen species and DNA damage induced by reactive oxygen species and play an important role in the treatment of cancer. It can interfere with the oxygen control signal of cancer cell growth. It can also make chemotherapy It only acts on cancer cells and protects normal cells from damage. [0003] Encapsulation in liposomes can enhance the stability of vitamin E, prevent oxidative degradation, improve its water solubility and bioavailability, and has broad application prospects in the field of health food. As a new type of membrane-modified liposome, coated liposomes have the advantage of increasing the stability of the liposome bilayer membrane, thereby improving the stability of the li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K9/14A61K31/355A61K47/34A61K47/40A61K47/36A61P39/06A61P35/00A23L1/29A23L33/00A61K47/10A61K47/22A61K47/26A61K47/32A61K47/44
Inventor 熊华赵丽萍彭海龙白春清刘玉珍史苏华邓波
Owner NANCHANG UNIV
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