Glabridin freeze-dried liposome and preparation method thereof

Abstract

The invention relates to glabridin freeze-dried liposome and a preparation method thereof. The glabridin freeze-dried liposome comprises, by weight, 65-70 parts of soybean phospholipid, 30-35 parts of cholesterol, 0.5-3.0 parts of glabridin, 1000-1500 parts of anhydrous ethanol and 70-120 parts of a phosphate buffer. The preparation method comprises the following steps of 1, weighing soybean phospholipid and cholesterol, putting the soybean phospholipid and cholesterol in an eggplant-shaped bottle, and dissolving the soybean phospholipid and cholesterol in anhydrous ethanol, 2, carrying out decompression on the eggplant-shaped bottle to evaporate the solvent, 3, preparing a glabridin solution, 4, preparing a glabridin phosphate solution, 5, preparing a glabridin freeze-dried liposome suspension, 6, preparing glabridin freeze-dried liposome by a freeze-drying method, and 7, carrying out vacuum-pumping by a freeze-drier for 5-10min so that water is completely evaporated and the glabridin freeze-dried liposome is obtained. The glabridin freeze-dried liposome has a reasonable design, has very strong external transdermal absorption performances and provides experiment basis for a bio-pharmaceutical industry.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a preparation method of glabridin freeze-dried liposome with excellent transdermal performance. Background technique [0002] With the rapid development of science and technology, instruments and methodologies, people have a deeper understanding of themselves, and the research on skin physiology and pathology has penetrated to the level of genes and molecular biology, which has had a huge impact on the development of related biomedical industries. As a driving force, many biologically active animal and plant components and biopolymer components have been applied to the biomedical industry and have achieved huge social and economic benefits. [0003] However, how to apply these biologically active ingredients to the field of biomedicine faces many difficulties, mainly in the following aspects: 1. The stability of these biologically active substances is determined by their own structure ...

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Problems solved by technology

[0003] However, how to apply these biologically active ingredients to the field of biomedicine faces many difficulties, mainly in the following aspects: 1. The stability of these biologically active substances is determined by their own structure and spatial conformation , the influence of external environmental factors should not be underestimated. For example, the active factor solution of the epidermis has a short duration of activity in the external environment for a few hours, and it is basically inactivated before it is absorbed by the skin and cannot play its role at all. Some functions and effects

2. The problem that polymer components are difficult to be absorbed by human skin. Generally speaking, the smaller the molecular weight of the active substance, the easier it is to penetrate the skin. Once the molecular weight is greater than 600, the active substance is difficult to be absorbed by the skin. In recent years, the active substance has Most of the substances with specific skin active functions are macromolecular substances such as peptides, and their molecular weights range from thousands to hundreds of thousands, which determines that they are difficult to penetrate through the skin and be effectively absorbed

3. The solubility of biologically active substances, most of the active ingredients cannot be dissolved in water and absorbed by the skin surface, it is difficult to play their role

[0004] Glabridin can accelerate blood flow, promote sweating, and take away excess heat in the body. It has the effects of detoxification, beauty, and weight loss. However, due to the above reasons, it is difficult to be absorbed by the skin and it is difficult to play its due role.

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